Effect of reactor surface on production of bacterial cellulose and water soluble oligosaccharides by Gluconacetobacter hansenii PJK

Abstract

The effect of agar plates on the bacterial cellulose (BC) production in a static culture was investigated in order to find the role of agar component as a surface modifying agent. Two types of surface modified reactors (SMRs: SMRD and SMRB) were prepared by coating the bottom of the reactors with agar dissolved in distilled water and basal medium, respectively. The SMRs were used for BC and water soluble oligosaccharides (WSOS) production. Control was done by the same procedure using reactors without agar plate. In both types of SMRs, the maximum production rate was observed after the second day of cultivation compared to third day of cultivation in the case of the control. The maximum productions of BC 5.308 and 5.472 g/L were observed at the first batch using SMRs prepared with agar dissolved in distilled water (SMRDs) and SMRs prepared with agar dissolved in a basal medium (SMRBs), respectively. Similarly, in the daily-culture and successive batch strategy experiments the maximum amount of WSOS produced in the SMRs was almost double that of the control. The highest water holding capacity value 92.21 g/g was observed for BC formed in the SMRs prepared with 3.0% of agar. FTIR and XRD analyses were carried out to study the structural features of the prepared BC. © KSBB hÉóïçêÇëW=Ä~ÅíÉêá~ä=ÅÉääìäçëÉI=ï~íÉê=ëçäìÄäÉ=çäáÖçë~ÅÅÜ~êáÇÉëI=~Ö~ê=éä~íÉëI=éêçÇìÅíáçå=~åÇ=éêçÇìÅíáîáíó= = = = =

Production and characterization of bacterial cellulose by Leifsonia sp. CBNU-EW3 isolated from the earthworm, Eisenia fetida

“…The fraction Iα and Iβ mixture of allomorphs was attributed to the cultivation condition of the bacteria and also the ratio of cellulose, which may differ according to bacterial strain. All of these results were consistent with earlier published X-ray data for BC obtained with other bacteria [7,25,26,[29][30][31][32][33][34]. However, compared with other bacteria, the peaks of BC produced by Leifsonia sp.…”

Time Dependent Influence of Rotating Magnetic Field on Bacterial Cellulose

“…It was reported by several authors that the loose fibril arrangement and large size of pores enhance the water capacity of BC [70,71]. It is considered that closely arranged microfibrils bind the water molecules more efficiently due to the stronger hydrogen bonding interactions, as compared to the loosely arranged microfibrils [72,73]. Furthermore, it was demonstrated that more water can be retained due to the larger surface area provided by thinner and longer fibers [74].…”

Effect of chitosan penetration on physico-chemical and mechanical properties of bacterial cellulose

“…The increase in Ch incorporation over the treatment time shown in the SEM analysis increased the WHC of the BC-Ch composite. The increased penetration of Ch results in better hydrogen bonding interaction between the BC and Ch molecules, which is also a factor causing an increase in the water holding capacity [30]. Nearly the same WHC for the BC-Ch composites prepared with 20 or 40 hrs of treatment indicates that the BC was almost saturated with Ch in 20 hrs of treatment.…”