Effect of recombinant adeno-associated virus vector-mediated vascular endothelial growth factor gene transfer on wound healing after burn injury*

Galeano, Mariarosaria; Deodato, Barbara; Altavilla, Domenica; Squadrito, Giovanni; Seminara, Paolo; Marini, Herbert; d’Alcontres, Francesco Stagno; Mr, Colonna; Calò, Margherita; Cascio, Patrizia Lo; Torre, Valerio; Giacca, Mauro; Venuti, Francesco S.; Squadrito, Francesco

doi:10.1097/01.ccm.0000059435.88283.c2

Cited by 54 publications

(30 citation statements)

References 45 publications

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“…32 The group confirmed these findings in a rat burn model, using the same gene and vector and demonstrating an increased epithelium regeneration and neo-angiogenesis. 33 However, the authors attributed the increased vascularization to the excellent tropism of the vector to the skeletal muscle layer underlying the skin in rodents. This thin muscle layer is not available in humans and generally causes wounds in the rat to heal faster, indicating that this vector might not be as potent when used in humans.…”

Section: Viral Gene Transfermentioning

confidence: 99%

“…20 Adeno-associated viral vectors have been studied intensively and are currently used in phase 1 clinical trials to treat cystic fibrosis and a-antitrypsin deficiency in adults. 30,31 Deodato et al 32 and Galeano et al 33 developed a model for external gene delivery of vascular endothelial growth factor A by the serotype 2 of adenoassociated viruses. Wound healing in the rat showed significant acceleration in 6-10 days after surgery (measurements of wound area reduction) and a wellstructured granulation and greater vascularization after 18 days in the treatment group compared to the control.…”

Section: Viral Gene Transfermentioning

confidence: 99%

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Gene therapy in wound healing: present status and future directions

et al. 2006

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Gene therapy was traditionally considered a treatment modality for patients with congenital defects of key metabolic functions or late-stage malignancies. The realization that gene therapy applications were much vaster has opened up endless opportunities for therapeutic genetic manipulations, especially in the skin and external wounds. Cutaneous wound healing is a complicated, multistep process with numerous mediators that act in a network of activation and inhibition processes. Gene delivery in this environment poses a particular challenge. Numerous models of gene delivery have been developed, including naked DNA application, viral transfection, high-pressure injection, liposomal delivery, and more. Of the various methods for gene transfer, cationic cholesterol-containing liposomal constructs are emerging as a method with great potential for non-viral gene transfer in the wound. This article aims to review the research on gene therapy in wound healing and possible future directions in this exciting field.

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Section: Viral Gene Transfermentioning

confidence: 99%

Section: Viral Gene Transfermentioning

confidence: 99%

Gene therapy in wound healing: present status and future directions

et al. 2006

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“…152 Matrix turnover significantly influences cellular phenotypic characters which in turn alters assembly of de novo synthesized matrix elements. Tissue remodeling in a variety of patho/physiological processes including embryogenesis, 153 normal tissue development, cancer 154 and wound healing 155,156 has been evaluated. These studies implicate an array of molecules regulating the process which are regulated at transcriptional, translational and post-translation levels.…”

Section: Matrix Turnovermentioning

confidence: 99%

Cell colonization in degradable 3D porous matrices

Lawrence

Madihally

2008

Cell Adhesion & Migration

178

155

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Cell colonization is an important in a wide variety of biological processes and applications including vascularization, wound healing, tissue engineering, stem cell differentiation and biosensors. During colonization porous 3D structures are used to support and guide the ingrowth of cells into the matrix. In this review, we summarize our understanding of various factors affecting cell colonization in three-dimensional environment. The structural, biological and degradation properties of the matrix all play key roles during colonization. Further, specific scaffold properties such as porosity, pore size, fiber thickness, topography and scaffold stiffness as well as important cell material interactions such as cell adhesion and mechanotransduction also influence colonization.

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“…19 Although these positive effects of vascular endothelial growth factor have been described, the exogenous administration of vascular endothelial growth factor for protection against the endothelial damage may often produce leaky, inflamed, and malformed vessels. 20,21 To the contrary, Ang1 is a specific growth factor with potential therapeutic applications in inducing angiogenesis, preventing vascular leakage, and inhibiting the apoptosis of endothelial cells to oxidative stress through several pathways that include phosphatidylinositol-3 kinase/AKT activation and the up-regulation of survivin protein.…”

Section: Discussionmentioning

confidence: 99%

Cartilage Oligometric Matrix Protein-Angiopoietin-1 Promotes Revascularization Through Increased Survivin Expression in Dermal Endothelial Cells of Skin Grafts in Mice

Byun

Choi

Park

et al. 2007

The American Journal of Pathology

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The present study examined the effects of cartilage oligometric matrix protein angiopoietin-1 (COMP-Ang1) on the revascularization of mice skin grafts. Full-thickness skin grafts were autotransferred into BALB/c mice. The donor grafts were soaked in COMP-Ang1 protein (50 g/ml, n ‫؍‬ 10) or in bovine serum albumin (BSA) (50 g/ml, n ‫؍‬ 10) dissolved in 1 ml of sterile, phosphate-buffered saline for 5 minutes before transfer. Revascularization of the grafts was monitored using an intravital microscope on postoperative days 3, 4, and 5. Morphological and immunohistochemical analyses were performed to evaluate platelet-endothelial cell adhesion molecule-1 and survivin expression and apoptotic signal in the transplanted grafts. Grafts soaked in COMP-Ang1 (COMP-Ang1 group) showed significantly increased revascularization compared with grafts soaked in BSA (BSA group) on intravital microscopy and plateletendothelial cell adhesion molecule-1 staining. The COMPAng1 group showed a significant increase of survivin expression in the endothelial cells and a reduction of apoptotic signal in comparison to the BSA group. Therefore, we believe that COMP-Ang1 provides the therapeutic benefit of enhancing the survival of vascular endothelial cells during transplantation of skin graft. (Am J Pathol

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Effect of recombinant adeno-associated virus vector-mediated vascular endothelial growth factor gene transfer on wound healing after burn injury*

Abstract: Our study suggests that rAAV-VEGF gene transfer may be an effective therapeutic approach to improve clinical outcomes after thermal injury.

Cited by 54 publications

References 45 publications

Gene therapy in wound healing: present status and future directions

Gene therapy in wound healing: present status and future directions

Cell colonization in degradable 3D porous matrices

Cartilage Oligometric Matrix Protein-Angiopoietin-1 Promotes Revascularization Through Increased Survivin Expression in Dermal Endothelial Cells of Skin Grafts in Mice

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