Effect of recombinant beet necrotic yellow vein virus with different RNA compositions on mechanically inoculated sugarbeets

Koenig, R.; Jarausch, W.; Li, Y.; Commandeur, Ulrich; Burgermeister, W.; Gehrke, Matthew; Lüddecke, P.

doi:10.1099/0022-1317-72-9-2243

Cited by 55 publications

(31 citation statements)

References 11 publications

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“…1). RNA 3 has a pronounced influence on the degree of symptoms in leaves of test plants and in sugarbeets (Kuszala et al, 1986;Tamada et al, 1989;Jupin et al, 1992;Koenig et al, 1991). RNA 4 seems to be involved in the transmission of the virus by Polymyxa betae (Tamada & Abe, 1989).…”

Section: Resultsmentioning

confidence: 99%

Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus

Kruse¹,

Koenig²,

Hoffmann³

et al. 1994

Journal of General Virology

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Beet necrotic yellow vein virus (BNYVV)-infected sugarbeets were obtained from many parts of Europe and also from some sites in Asia and the U.S.A. Reverse transcription (RT)-PCR products of more than I kbp were obtained for four different regions of the viral genome which may be particularly important with respect to the pathogenic properties of the virus, i.e. for the coat protein and the 42K protein-encoding regions on RNA 2 and for major parts ofRNAs 3 and 4. Restriction fragment length polymorphism (RFLP) patterns obtained with these PCR products revealed the existence of two major strain groups of BNYVV, named type A and type B. The A type was detected in Greece, the former Yugoslavia, Slovakia, parts of Austria, Italy, Spain, parts of France, Belgium, The Netherlands and England as well as in Asia (Turkey, Kazachstan, China and Japan) and the U.S.A. The B type occurs in Germany and parts of France. Mixed infections were detected at the borderline regions between areas of the A and B types. Comparisons of published and newly determined nucleotide sequences of the respective parts of the BNYVV genome indicate that the percentage of nucleotide differences between the A and the B type is approximately 3 % for the respective regions of RNAs 2 and 3 and approximately 1.5 % for RNA 4. Nucleotide sequences appear to be remarkably stable within each of the two strain groups. The majority of the nucleotide differences between the A and B types occur in the third triplet position. The amino acid changes in the coat protein area are outside the four previously determined antigenic regions that are accessible on the surface of the virus particles and are involved in the formation of continuous and presumably also discontinuous epitopes. This may explain why serological differences between the two strain groups have not been found.

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Section: Resultsmentioning

confidence: 99%

Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus

Kruse¹,

Koenig²,

Hoffmann³

et al. 1994

Journal of General Virology

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“…BVQ was found in Bulgaria, Belgium, France, Germany, Hungary, Italy, and The Netherlands, but it was not found in Turkey. From an epidemiological point of view, although the role of BNYVV in rhizomania syndrome is well established (21,22), there is controversy regarding the role of P. betae in seedling growth (4) and in severe stunting of sugar beet (18) and still others reject the notion that P. betae has any effect on sugar beet growth (22). The controversy is emphasized by the study of two other viruses, which were possibly undetected in the P. betae used in previous studies (17).…”

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confidence: 88%

Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus , Beet Soilborne Virus , and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

Meunier

Schmit

Stas

et al. 2003

Appl Environ Microbiol

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Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and BVQ, together with their vector, P. betae. The detection threshold of the test was up to 128 times greater than that of an enzyme-linked immunosorbent assay. Systematic association of BNYVV with one or two different pomoviruses was observed. BVQ was detected in samples from Belgium,

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“…The viral genome consists of four positive-sense RNA species (Bouzoubaa et al, 1985(Bouzoubaa et al, , 1987. BNYVV is transmitted in the field by the plasmodiophoromycete Polymyxa betae, which is associated with roots and, under these conditions, all four RNAs are required for infection (Koenig et al, 1986(Koenig et al, , 1991Tamada et al, 1990). The virus can also be transmitted to leaves of some hosts, principally Chenopodiacae, by mechanical inoculation (Tamada, 1975).…”

Section: Introductionmentioning

confidence: 99%

“…Under these circumstances, only RNAs 1 and 2 are required for infection indicating that RNAs 1 and 2 direct basic functions required in all hosts (Koenig et al, 1986;Quillet et al, 1989;Tamada et al, 1989). RNA 4 has been shown to be important for efficient transmission of the virus by its fungal vector (Tamada & Abe, 1989) and RNA 3 appears to play a role in the replication and/or translocation of the virus in roots (Tamada et al, 1990;Koenig et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

In situ localization of the non-structural protein P25 encoded by beet necrotic yellow vein virus RNA 3

Haeberlé

Stussi-Garaud

1995

Journal of General Virology

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The in situ localization of the non-structural protein P25 encoded by beet necrotic yellow vein virus (BNYVV) RNA 3 and of the BNYVV coat protein (CP) was studied by immunoelectron microscopy in infected leaf and root cells of Chenopodium murale and C. quinoa. The CP was detected in the cytoplasm of all cell types except xylem, sieve elements, and companion cells. P25 was detected in the cytoplasm and nuclei of the same cell types. The intensity of CP labelling varied depending upon the stage of infection of the cell, whereas the P25 labelling intensity was similar in newly infected cells and in cells at later stages of infection. These results suggest that P25 may be synthesized at an earlier stage of infection than CP. Its presence in the nuclei of newly infected cells may be related to the reported effect of P25 on leaf symptom development.

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Effect of recombinant beet necrotic yellow vein virus with different RNA compositions on mechanically inoculated sugarbeets

Cited by 55 publications

References 11 publications

Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus

Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus

Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus , Beet Soilborne Virus , and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

In situ localization of the non-structural protein P25 encoded by beet necrotic yellow vein virus RNA 3

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