Effect of Recombinant Bovine Somatotropin on Milk Production and Composition of Cows with Streptococcus uberis Mastitis

Hoeben, Dagmar; Burvenich, Christian; Eppard, P.J.; Hard, D.L.

doi:10.3168/jds.s0022-0302(99)75396-8

Cited by 15 publications

(15 citation statements)

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“…Somatotropin protected the mammary gland from excessive production losses. It also significantly improved the normalisation of composition, indicating a beneficial effect on the restoration of the integrity of the blood-milk barrier [100]. Also in this model, rbST treatment induced significantly higher IGF-I and rbST concentrations in plasma.…”

Section: Effect Of Rbst On Milk Loss During Mastitismentioning

confidence: 56%

“…During severe clinical coliform mastitis a decrease in total milk yield occurs. It Severity of E. coli mastitis in dairy cows 537 is not surprising that milk production was also reduced in uninfected quarters during coliform mastitis [59,93,99,100,102,152,167,210,245]. The magnitude of this reduction is the result of: (1) a local effect induced by the invading pathogen and in which inhibition of milk synthesis and tissue damage is involved, and (2) the degree of systemic disease; e.g.…”

Section: Systemic Disease Parametersmentioning

confidence: 99%

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Severity of E. coli mastitis is mainly determined by cow factors

et al. 2003

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-Intramammary infections of dairy cows with Gram-positive bacteria such as Staphylococcus aureus (major cause of mastitis) have received a lot of attention because of their major economic impact on the dairy farm through production losses induced by an increase in somatic cell count. Management strategies, including greater awareness for efficient milking and hygienic measures, have limited the spread of Gram-positive bacteria and resulted in a significant decrease of proportion of S. aureus isolates and subclinical mastitis worldwide. Other organisms such as coliform subspecies and Streptococcus uberis, both environmental bacteria that cause clinical mastitis, have received less attention. Escherichia coli causes inflammation of the mammary gland in dairy cows around parturition and during early lactation with striking local and sometimes severe systemic clinical symptoms. This disease affects many high producing cows in dairy herds and may cause several cases of death per year in the most severe cases. It is well known that bacterial, cow and environmental factors are interdependent and influence mastitis susceptibility. Many studies, executed during the last decade, indicate that the severity of E. coli mastitis is mainly determined by cow factors rather than by E. coli pathogenicity. During E. coli mastitis, the host defense status is a cardinal factor determining the outcome of the disease. Today, we know that the neutrophil is a key factor in the cows' defense against intramammary infection with E. coli. Effective elimination of the pathogen by neutrophils is important for the resolution of infection and the outcome of E. coli mastitis. This review is a compilation of some major findings over the last 15 years concerning mainly host factors that modulate and influence neutrophil function and the mammary inflammatory reaction. The individual chapters address: virulence factors of E. coli strains, how neutrophils kill E. coli, connection between endotoxins, tumor necrosis factor-a and nitric oxide, severity classification of E. coli mastitis, lifespan of neutrophils, host factors that influence severity, tissue damage and production loss.

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Section: Effect Of Rbst On Milk Loss During Mastitismentioning

confidence: 56%

Section: Systemic Disease Parametersmentioning

confidence: 99%

Severity of E. coli mastitis is mainly determined by cow factors

et al. 2003

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“…Recombinant bST has been shown to protect cows from excessive milk production losses and compositional changes during a subsequent, experimentally induced Escherichia coli and Streptococcus uberis mastitis (25,49). Recombinant bST also prevented severe local and general clinical symptoms in cows suffering from E. coli mastitis, especially in severe responders.…”

Section: Introductionmentioning

confidence: 99%

Effect of Bovine Somatotropin on Neutrophil Functions and Clinical Symptoms During Streptococcus uberis Mastitis

Hoeben¹,

Burvenich²,

Eppard³

et al. 1999

Journal of Dairy Science

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The effect of recombinant bovine somatotropin (bST) on the chemiluminescence, diapedesis, and expression of adhesion receptors (CD11a, CD11b, CD18) of isolated polymorphonuclear leukocytes was studied. The plasma concentrations of insulin-like growth factor-I (IGF-I), bST, cortisol, and alactalbumin were also monitored. In addition, general and local clinical symptoms and the differentiation of circulating leukocytes were also studied during experimentally induced Streptococcus uberis mastitis in cows. Ten cows were infected with 500 cfu of S. uberis O140J in both left quarters. Five cows were subcutaneously treated with 500 mg of recombinant bST 7 d before and after infection, and 5 control cows received the excipient. General (fever, tachycardia, inappetance, and depression) and local symptoms (swelling, pain, firmness, and flecks in milk) were more acute, severe, and longer-lasting in control cows. Treatment with bST had no effect on chemiluminescence and diapedesis of circulating polymorphonuclear leukocytes and no effect on the expression of adhesion receptors. Recombinant bST induced significantly higher IGF-I and bST concentrations in plasma. The leukopenia observed after infection was less pronounced in the bST-treated cows, and the number of circulating band neutrophils and metamyelocytes was significantly lower in the treated group. The concentration of cortisol did not differ between both groups, but the blood concentration of alactalbumin significantly increased in both groups from 6 d after infection. These results showed that treatment with recombinant bST improves animal welfare by protecting the cows from severe local and general clinical symptoms during subsequent S. uberis mastitis, but that it has no effect on chemiluminescence, diapedesis, and the expression of adhesion receptors of circulating polymorphonuclear leukocytes. ( Key words: Streptococcus uberis mastitis, recombinant bovine somatotropin, diapedesis, chemiluminescence)Abbreviation key: AUC = area under the curve, CL = chemiluminescence, DPBS = Dulbecco's PBS solution, HR = heart rate, MFI = mean fluorescence intensity, PMA = phorbol 12-myristate 13-acetate, PMN = polymorphonuclear leukocytes, RT = rectal temperature, ST = somatotropin, TNF-a = tumor necrosis factor alpha.

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“…According to previous studies, both clinical and subclinical mastitis can affect the composition and manufacturing properties of milk (Auldist and Hubble, 1998;Pyörälä, 2003). The most common effect reported is the dramatic increase of Na + concentration and decrease of K + concentration (Vandeputte- Van et al, 1993;Auldist et al, 1995;Hoeben et al, 1999). To some extent, variation in the ion concentrations might be correlated with the inhibition of ATPase activity of the iron-transporting system.…”

Section: Introductionmentioning

confidence: 99%