Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro

Kuzmina, T. I.; Alm, H.; Denisenko, V. Yu.; Tuchscherer, Armin; Kanitz, W.; Torner, H.

doi:10.1262/jrd.18116

Cited by 16 publications

(13 citation statements)

References 34 publications

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“…Moreira et al [144]; Bevers and Izadyar [145]; Kölle et al [146,147]; Pozzobon et al [148]; Kuzmina et al [149]; Velazquez et al [150,151]; Makarevich et al [152] IGF-I, IGF-II, FGF2, transforming growth factor beta1 (TGF β1 ), leukemia inhibitory factor (LIF) and CSF2, singly and in combination.…”

Section: The Use Of Alternative Macromoleculesmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

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Despite advantages of in vitro embryo production in many species, widespread use of this technology is limited by generally lower developmental competence of in vitro derived embryos compared to in vivo counterparts. Regardless, in vivo or in vitro gametes and embryos face and must adjust to multiple microenvironments especially at preimplantation stages. Moreover, the embryo has to be able to further adapt to environmental cues in utero to result in the birth of live and healthy offspring. Enormous strides have been made in understanding and meeting stage-specific requirements of preimplantation embryos, but interpretation of the data is made difficult due to the complexity of the wide array of culture systems and the remarkable plasticity of developing embryos that seem able to develop under a variety of conditions. Nevertheless, a primary objective remains meeting, as closely as possible, the preimplantation embryo requirements as provided in vivo. In general, oocytes and embryos develop more satisfactorily when cultured in groups. However, optimization of individual culture of oocytes and embryos is an important goal and area of intensive current research for both animal and human clinical application. Successful culture of individual embryos is of primary importance in order to avoid ovarian superstimulation and the associated physiological and psychological disadvantages for patients. This review emphasizes stage specific shifts in embryo metabolism and requirements and research to optimize in vitro embryo culture conditions and supplementation, with a view to optimizing embryo culture in general, and culture of single embryos in particular.

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Section: The Use Of Alternative Macromoleculesmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

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“…Only the labelled mitochondria that were actively respiring were recorded. The fluorescence intensity per oocyte (mA) was measured by the Nikon Photometry System P 100 (Nikon, Düsseldorf, Germany) as described in pig, horse and bovine oocytes (Torner et al 2004, 2007, Kuzmina et al 2007. For measurement of intensity, we placed the whole oocyte (thickness around 20 mm) with the eyepiece of the Photometer head P 100 in a defined area of measurement (same size for all observation).…”

Section: Oocyte Analysismentioning

confidence: 99%

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

Torner¹,

Ghanem²,

Ambros³

et al. 2008

Reproduction

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Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. However, the intrinsic molecular and subcellular characteristics of these oocytes have not yet been investigated. Here, we aim to identify molecular and functional markers associated with oocyte developmental potential when selected based on G6PDH activity. Immature compact cumulus-oocyte complexes were stained with brilliant cresyl blue (BCB) for 90 min. Based on their colouration, oocytes were divided into BCB K (colourless cytoplasm, high G6PDH activity) and BCB C (coloured cytoplasm, low G6PDH activity). The chromatin configuration of the nucleus and the mitochondrial activity of oocytes were determined by fluorescence labelling and photometric measurement. The abundance and phosphorylation pattern of protein kinases Akt and MAP were estimated by Western blot analysis. A bovine cDNA microarray was used to analyse the gene expression profiles of BCB C and BCB K oocytes. Consequently, marked differences were found in blastocyst rate at day 8 between BCB C (33.1G3.1%) and BCB K (12.1G1.5%) oocytes. Moreover, BCB C oocytes were found to show higher phosphorylation levels of Akt and MAP kinases and are enriched with genes regulating transcription (SMARCA5), cell cycle (nuclear autoantigenic sperm protein, NASP ) and protein biosynthesis (RPS274A and mRNA for elongation factor 1a, EF1A). BCB K oocytes, which revealed higher mitochondrial activity and still nucleoli in their germinal vesicles, were enriched with genes involved in ATP synthesis (ATP5A1), mitochondrial electron transport (FL405), calcium ion binding (S100A10) and growth factor activity (bone morphogenetic protein 15, BMP15). This study has evidenced molecular and subcellular organisational differences of oocytes with different G6PDH activity.

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“…It is known that oocytes require maturation of both cytoplasm and nucleus to be able to support successful fertilization and subsequent embryo development (Kuzmina et al, 2007;Trounson et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Maturational gene upregulation and mitochondrial activity enhancement in mouse in vitro matured oocytes and using granulosa cell conditioned medium

Zand

Fathi

Nasrabadi

et al. 2018

Zygote

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SummaryThe high miscarriage rates that result following transfer of embryos derived from in vitro maturation (IVM) of oocytes necessitate improvements in the processes involved. This study aimed to improve the quality of in vitro matured oocytes using granulosa cell conditioned medium (GCCM) as the culture medium. In this work, germinal vesicle (GV)-stage oocytes from NMRI mice were collected and cultured using three types of culture medium: Base medium (BM) (control), 50% granulosa cell conditioned medium (GCCM50) and 100% GCCM (GCCM100). After IVM, the mitochondria activity potential and viability of metaphase II (MII) oocytes were evaluated by JC-1 and trypan blue staining, respectively. Maturational gene expression levels of CyclinB1, Cdk1 and Gdf9 in the control, GCCM50 and GCCM100 samples were analyzed using real-time polymerase chain reaction (PCR). The viability rate of in vitro matured oocytes was highest in the GCCM50 group. JC-1 staining showed that GCCM50 enhances mitochondrial activity more than the other groups (P < 0.05). Gene expression levels of Cdk1 and Gdf9 were higher in the group with GCCM50 treatment, than in the control and GCCM100 groups (P < 0.05), while the expression level of CyclinB1 did not differ among the groups. The results indicated that a 50% concentration of GCCM in combination with BM components enhanced MII and viability rates and mitochondria activity of mouse immature oocytes.

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Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro

Cited by 16 publications

References 34 publications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

Maturational gene upregulation and mitochondrial activity enhancement in mouse in vitro matured oocytes and using granulosa cell conditioned medium

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