Effect of recombinant gonadotropins on embryo quality in superovulated rabbit does and immune response after repeated treatments

Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

“…Regarding to superovulatory response, both purified pFSH administered in five injections, twice a day (pFSH5), and rhFSH dissolved in PVP administered in three injections, once a day for three days (rhFSH3), resulted in a higher ovulation rate than control group. However, in spite of their ability to induce a superovulation response, while rhFSH reached similar results to those reported previously with oFSH, pFSH and rhFSH, increasing two times the ovulation rate and the number of oocytes or embryos recovered in superovulated does (Kauffmann et al, 1998;Viudes de Castro et al, 2009), the pFSH5 treatment, showed a ovulation rate 3.5 times higher than control group. Despite an increase in the number of oocytes produced per female, when animals are treated with pFSH5 protocol, the blastocyst developmental rate per oocyte was significantly decreased compared to control females.…”

“…Given that it has been observed that FSH in absence of LH could induce superovulatory response in rabbit does both using purified porcine FSH or recombinant human FSH Cortell et al, 2008;Viudes de Castro et al, 2009), in the present study, the effect of these hormonal preparations on ovulation rate and oocyte quality was compared. Regarding to superovulatory response, both purified pFSH administered in five injections, twice a day (pFSH5), and rhFSH dissolved in PVP administered in three injections, once a day for three days (rhFSH3), resulted in a higher ovulation rate than control group.…”

“…In rabbits, superovulation protocols have been performed widely with eCG or FSH (Kauffmann et al, 1998;Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010). Nevertheless, the recovery efficiency and oocyte quality of these superovulation treatments are highly variables.…”

“…However, in order to limit animal manipulation, superovulation treatments with FSH diluted in polyvinylpyrrolidone (PVP) solutions have been tested and have resulted in good superovulation response in distinct species, including rabbits Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010).…”

“…In rabbits, the effect of LH on superovulation has been studied using both porcine purified FSH and human recombinant FSH (Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010). In all cases, superovulatory response has been achieved; however, it would be very useful to determine how oocyte quality may be affected by these superovulation procedures.…”