Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability

Accardo, C; Dattena, Maria; Pilichi, Susanna; Ladu, Mara; Chessa, Bernardo; Cappai, P.

doi:10.1016/j.anireprosci.2003.10.004

Cited by 50 publications

(36 citation statements)

References 41 publications

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“…It is likely that steroids, gonadotrophins and the maturation medium itself all play an important role, particularly if the oocytes are matured in defined or semi-defined culture media (Accardo et al, 2004). Follicle-stimulating hormone (FSH) maintained associations between growing oocytes and cumulus cells during in vitro growth culture, probably by supporting the oocytes (Cayo-Colca et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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-This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 -100% FF) slowed meiotic progression and CG migration (control -78.13% vs. treated -52.58% and control -52.7% vs. treated -11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control -90.13% vs. treated -82.64% and control -35.73% vs. treated -11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control -29.91 vs. treated -35.49, at 24 h of maturation) and total cell numbers (control -109.53 vs. treated -120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

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Section: Resultsmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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“…Following a gonadotropin-independent phase, the mammalian follicular growth first becomes FSH-dependent at the secondary stage and then LH-dependent prior to ovulation [8-12]. While FSH is mainly involved in follicular growth, cellular proliferation and oestrogen production (aromatase activity), LH induces androgen biosynthesis, final maturation of the oocyte and ovulation [13-19]. To achieve their functions, FSH and LH trigger multiple downstream cascades of intra-ovarian pathways that are necessary for proper female fertility [13,14,17,20-23].…”

Section: Introductionmentioning

confidence: 99%

“…While FSH is mainly involved in follicular growth, cellular proliferation and oestrogen production (aromatase activity), LH induces androgen biosynthesis, final maturation of the oocyte and ovulation [13-19]. To achieve their functions, FSH and LH trigger multiple downstream cascades of intra-ovarian pathways that are necessary for proper female fertility [13,14,17,20-23]. In addition, it has been shown that the efficiency of most assisted reproductive technologies (ART) used in both humans and livestock, including ovarian stimulation and oocyte in vitro maturation (IVM), relies on gonadotropin input [24-31].…”

Section: Introductionmentioning

confidence: 99%

FSH in vitro versus LH in vivo: similar genomic effects on the cumulus

2013

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The use of gonadotropins to trigger oocyte maturation both in vivo and in vitro has provided precious and powerful knowledge that has significantly increased our understanding of the ovarian function. Moreover, the efficacy of most assisted reproductive technologies (ART) used in both humans and livestock species relies on gonadotropin input, mainly FSH and LH. Despite the significant progress achieved and the huge impact of gonadotropins, the exact molecular pathways of the two pituitary hormones, FSH and LH, still remain poorly understood. Moreover, these pathways may not be the same when moving from the in vivo to the in vitro context. This misunderstanding of the intricate synergy between these two hormones leads to a lack of consensus about their use mainly in vitro or in ovulation induction schedules in vivo. In order to optimize their use, additional work is thus required with a special focus on comparing the in vitro versus the in vivo effects. In this context, this overview will briefly summarize the downstream gene expression pathways induced by both FSH in vitro and LH in vivo in the cumulus compartment. Based on recent microarray comparative analysis, we are reporting that in vitro FSH stimulation on cumulus cells appears to achieve at least part of the gene expression activity after in vivo LH stimulation. We are then proposing that the in vitro FSH-response of cumulus cells have similitudes with the in vivo LH-response.

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“…The formulation of a defined maturation medium (DMM: medium not containing serum or follicular fluid) might help to further elucidate the metabolic and nutritional requirements of mammalian oocytes and embryos produced in vitro (4). In addition, to improve quality control and the capacity for comparing data among laboratories, it is highly desirable to completely optimize DMM for embryo production (3).…”

mentioning

confidence: 99%

Developmental potential of sheep oocytes cultured in different maturation media: effects of epidermal growth factor, insulin-like growth factor I, and cysteamine

Shabankareh

Zandi

2010

Fertility and Sterility

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Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability

Cited by 50 publications

References 41 publications

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

FSH in vitro versus LH in vivo: similar genomic effects on the cumulus

Developmental potential of sheep oocytes cultured in different maturation media: effects of epidermal growth factor, insulin-like growth factor I, and cysteamine

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