Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems

Yuan, Danni; Yao, Mimi; Wang, Lan; Li, Yanhua; Gong, Yingchun

doi:10.1016/j.algal.2019.101718

Cited by 26 publications

(6 citation statements)

References 39 publications

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“…The DOM mainly consisted of EPS. EPS could cause an increase in the viscosity of the medium and inhibit the growth of A. platensis , through the reduction in the quantum yield of photosystem II and the change in the biomass composition [ 14 ]. This might mainly contribute to the decreased biomass production of A. platensis as well.…”

Section: Resultsmentioning

confidence: 99%

“…Compared with the fresh medium, the growth rate of microalgae cultured in the recycled medium decreased with the increase in the number of recycle time of the medium [ 14 ], and the accumulated EPS may influence the rheological properties of the culture medium [ 15 ]. Moreover, the decomposition of the organic matter results in the increase in bacterial population; in particular, ciliates seem to thrive well under these conditions [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Culture Conditions on the Performance of Arthrospira platensis and Its Production of Exopolysaccharides

Liu

Zhou

et al. 2022

Foods

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Exopolysaccharides (EPS) produced by Arthrospira platensis (A. platensis) has been widely applied in industry and commerce for its various activities but the accumulation of EPS in culture medium may influence the growth of A. platensis reversely. This work aims to explore the impacts of initial pH, nitrogen source and concentration, phosphate concentration and recycle times of the culture medium on the growth of A. platensis and the secretion of its EPS. The results showed that EPS accumulated with the increase in recycle times of culture medium. The optimal initial pH for the growth of A. platensis was 8.50, and high pH of 11.5 inhibited the growth of biomass while resulting in highest EPS content of 92.87 mg/g DW. Excessive and limited nitrogen (NaNO3 of 25.00 g/L and NaNO3 < 2.50 g/L) and phosphate (K2HPO4 of 5.00 g/L and K2HPO4 < 0.50 g/L) inhibited the biomass production of A. platensis by 1.28–30.77% and 14.29–45.05%, respectively. EPS yield of 97.57 mg/g DW and 40.90 mg/g DW were obtained under NaNO3 of 25.00 g/L and K2HPO4 of 5.00 g/L due to salt stress. These findings are beneficial in providing a theoretical basis for high yield EPS from A. platensis without affecting biomass yield.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Culture Conditions on the Performance of Arthrospira platensis and Its Production of Exopolysaccharides

Liu

Zhou

et al. 2022

Foods

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“…Spirulina platensis merupakan organisme fotoautotrof yang melakukan proses fotosintesis untuk memperoleh energi yang diperlukan dalam metabolisme dan pertumbuhan. Fotosintesis dipengaruhi oleh berbagai variabel internal maupun eksternal yang selanjutnya akan berdampak terhadap produksi biomassa Spirulina (Yuan et al, 2019;Lim et al, 2021).…”

Section: Pertumbuhan Spirulina Platensisunclassified

Pertumbuhan, Kadar Pigmen dan Aktivitas Antioksidan Spirulina platensis pada Kultur dengan Perbedaan Warna Pencahayaan Leds

2022

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Antioksidan diperlukan tubuh untuk melindungi kesehatan kulit kita. Salah satu sumber antioksidan adalah mikroalga Spirulina sp. Permasalahan pemanfaatan spirulina adalah diperlukan teknologi kultur yang tepat untuk menghasilkan aktivitas antioksidan tinggi. Tujuan penelitian adalah melakukan optimasi pencahayaan (warna cahaya) dalam kultur spirulina untuk mendapatkan pertumbuhan terbaik, kadar pigmen dan aktivitas antioksidan tertinggi. Penelitian menggunakan metode eksperimen laboratoris dengan 5 perlakuan yaitu pemberian pencahaaan dengan LED warna biru, hijau, merah, putih dan lampu TL (fluoresens). Perlakuan perbedaan warna pencahayaan dalam kultur berpengaruh nyata terhadap pertumbuhan, kadar pigmen dan aktivitas antioksidan. Perlakuan terbaik adalah pemberian pencahayaan LED merah yaitu Spirulina platensis memiliki pertumbuhan mutlak sebesar 1,602 d x 104 sel/ml, laju pertumbuhan 0,108 hari-1, jumlah generasi 2,028, waktu generasi 6,416 hari, yield 9,649 gr/gr, dan produktifitas 24,740 gr/L.hari. Ekstrak spirulina mengandung klorofil a sebesar 10,153 mg/L, klorofil b sebesar 4,744 mg/L, total klorofil 16,053 mg/L, karotenoid 5,893 mg/L. selain itu juga memiliki kandungan pigmen fikobiliprotein yaitu fikosianin 0,055 mg/L, alofikosianin 0,037 mg/L dan fikoeritrin 0,218 mg/L. eksrak spirulina dari hasil kultur dengan pencahayaan LED merah memiliki aktivitas antioksidan dengan nilai IC50 sebesar 105,4 ppm. Aktivitas antioksidan dari ekstrak sepirulina di karenakan adanya kandungan senyawa fitokimia dan pigmen. Antioxidants are needed by the body to protect the health of our skin. One source of antioxidants is the microalgae spirulina sp. The problem of using spirulina is that appropriate culture technology is needed to produce high antioxidant activity. The aim of the research was to optimize lighting (light color) in spirulina culture to get the best growth, highest pigment content and antioxidant activity. The study used a laboratory experimental method with 5 treatments, lighting with blue, green, red, white LEDs and fluorescent lights. The treatment of different lighting colors in culture had a significant effect on growth, pigment levels and antioxidant activity. The best treatment was the provision of red LED lighting, Spirulina platensis which had absolute growth of 1.602 d x 104 cells/ml, growth rate of 0.108 day-1 , number of generations 2,028, generation time of 6.416 days, yield of 9.649 gr/gr, and productivity of 24.740 gr/L.day. Spirulina extract contains chlorophyll a of 10,153 mg/L, chlorophyll b of 4,744 mg/L, total chlorophyll 16,053 mg/L, carotenoids 5,893 mg/L. In addition, it also contains phycobiliprotein pigments, phycocyanin 0.055 mg/L, allophycocyanin 0.037 mg/L and phycoerythrin 0.218 mg/L. Spirulina extract from culture results with red LED lighting has antioxidant activity with an IC50 value of 105.4 ppm. The antioxidant activity of the sepirulina extract is due to the presence of phytochemical compounds and pigments.

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“…Media kultur yang digunakan adalah air laut hasil filtrasi dengan salinitas 30 ppt. Sebanyak 12 erlenmeyer ukuran 2 liter masing -masing di isi dengan air laut 30 ppt sebanyak 1,5 L, media walne sebanyak 1%, dan diberi aerasi (Yuan et al, 2019). Perlakuan yang didiberikan adalah perbedaan intensitas pencahayaan dalam kultur Spirulina platensis yaitu dengan pencahayaan lampu TL 23 watt (1500 lux), 30 watt (4000 lux), 36 watt (6500 lux) dan 45 watt (9000 lux).…”

Section: Materi Dan Metodeunclassified

Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan

2022

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Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein. Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. The DPPH radical scavenging activity of the extract was due to the presence of antioxidant compounds from chlorophyll, carotenoids and phycobiliproteins.

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Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems

Cited by 26 publications

References 39 publications

Effects of Culture Conditions on the Performance of Arthrospira platensis and Its Production of Exopolysaccharides

Effects of Culture Conditions on the Performance of Arthrospira platensis and Its Production of Exopolysaccharides

Pertumbuhan, Kadar Pigmen dan Aktivitas Antioksidan Spirulina platensis pada Kultur dengan Perbedaan Warna Pencahayaan Leds

Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan

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