Effect of red and infrared low-level laser therapy in endodontic sealer on subcutaneous tissue

Sivieri-Araújo, Gustavo; Berbert, Fábio Luiz Camargo Villela; Ramalho, Luciana Maria Pedreira; Rastelli, Alessandra Nara de Souza; Crisci, F.S.; Bonetti-Filho, Idomeo; Tanomaru-Filho, Mário

doi:10.1134/s1054660x11210201

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…In this study, rats were used as a model animal, because are extensively used and well known scientifically [31,32]. The experimental solutions (saline, 5% NaOCl and curcumin PS 500 mg/L), were insert into polyethylene tubes and implanted immediately into the animals, which allowed direct contact between the material and the Fig.…”

Section: Discussionmentioning

confidence: 99%

Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

Sivieri-Araújo

Queiroz

Dal‐Fabbro

et al. 2017

Photodiagnosis and Photodynamic Therapy

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Background: The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O 2 . The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods: Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1β and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results: All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1β levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions: aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1β and IL-6 cytokines production.

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Section: Discussionmentioning

confidence: 99%

Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

Sivieri-Araújo

Queiroz

Dal‐Fabbro

et al. 2017

Photodiagnosis and Photodynamic Therapy

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“…However, irradiation with Nd:YAG alone has not been able to sealing dentine surface of apicoetomies 13 , been able to clean root canals when used in combination with hand files showing mostly an absence of smear layer and tissue remnants 9 . GaAlAs lasers have also been used in endodontics 14 . However, only a few studies have reported about dentin treatment or smear layer removal 15 .…”

Section: Introductionmentioning

confidence: 99%

Two lasers x ultrasonic on root canal walls treatment: a scanning electron microscopy study

Berbert¹,

Pimenta²,

Dametto³

et al. 2022

J Multidiscip Dent

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The aim of this study was to assess the efficacy of smear layer/debris removal with EDTA, passive ultrasonic irrigation (PUI), Nd:YAG or GaAlAs laser, using surface topography analysis using scanning electron microscopy (SEM). Forty maxillary canines were decoronated, instrumented and randomly assigned (n = 10): G1 - 17% EDTA; G2 - PUI; G3 - Nd:YAG laser; G4 - GaAlAs laser. The roots were prepared for SEM and the cleanliness of each third of the root canal walls was accessed using a predetermined rating system. Kruskal-Wallis test was performed for statistical analysis. A significant difference was observed for G1 and the other three groups in the coronal third (p < 0.01), between G1, G2 and G4 (p < 0.01) and between G1 and G3 (p < 0.05) for the middle third, and between G1 and G4 (p < 0.01) and G1 and G2 (p < 0.05) in the apical third. Not one sample showed a perfectly clean surface of all levels of the root canal.

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“…Indeed, clinical protocols based on low fluences and power densities are recommended in device guidelines for treating various diseases on an empirical basis or based on clinical practice. At low fluences, infrared lasers are believed to induce photochemical interactions and interesting applications such as management of pain [6], wound healing [7][8][9], and treatment of inflammatory diseases [1,10,11] of nearinfrared lasers have been described.…”

Section: Introductionmentioning

confidence: 99%

Low intensity infrared laser affects expression of oxidative DNA repair genes in mitochondria and nucleus

et al. 2014

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Practical properties and physical characteristics of low intensity lasers have made possible their application to treat soft tissue diseases. Excitation of intracellular chromophores by red and infrared radiation at low energy fluences with increase of mitochondrial metabolism is the basis of the biostimulation effect but free radicals can be produced. DNA lesions induced by free radicals are repaired by the base excision repair pathway. In this work, we evaluate the expression of POLγ and APEX2 genes related to repair of mitochondrial and nuclear DNA, respectively. Skin and muscle tissue of Wistar rats were exposed to low intensity infrared laser at different fluences. One hour and 24 hours after laser exposure, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of POLγ and APEX2 mRNA expression by real time quantitative polymerase chain reaction. Skin and muscle tissue of Wistar rats exposed to laser radiation show different expression of POLγ and APEX2 mRNA depending of the fluence and time after exposure. Our study suggests that a low intensity infrared laser affects expression of genes involved in repair of oxidative lesions in mitochondrial and nuclear DNA.

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Effect of red and infrared low-level laser therapy in endodontic sealer on subcutaneous tissue

Cited by 8 publications

References 34 publications

Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy

Two lasers x ultrasonic on root canal walls treatment: a scanning electron microscopy study

Low intensity infrared laser affects expression of oxidative DNA repair genes in mitochondria and nucleus

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