Effect of reduction of cholinergic input on the concentration of specific proteins in different cortical regions of the rat brain

Heydorn, William E.; Nguyen, Khanh Q.; Creed, G. Joseph; Jacobowitz, David M.

doi:10.1016/0006-8993(85)90085-x

Cited by 10 publications

(1 citation statement)

References 34 publications

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“…The 2-DE procedure of O'Farrell [21) was followed with minor modifications [12,13). In the isoelectric focusing dimension.…”

Section: Polyacrylamide 2-dementioning

confidence: 99%

Incorporation of Amino Acids into Proteins of the Hypothalamus of Prepuberal Female Rats after Estradiol Treatment

Rodriguez-Sierra

et al. 1987

Neuroendocrinology

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The arcuate nucleus-median eminence complex (AM) undergoes major structural and functional changes during normal puberty or if exposed to a pulse of estradiol in the prepuberal period. Those changes are expressed by increased synaptogenesis and by a drastic alteration in the feedback control of anterior pituitary gland hormone release. In this study we investigated the effects of estradiol benzoate (EB) on specific proteins in this hypothalamic area. Prepuberal, 25-day-old female rats were administered 10 µg of EB s.c. in oil or sesame oil vehicle. The animals were decapitated either 17 or 42 h after treatment. The brains were removed, blocked and serially sections at 300 µm using a Vibratome. The AM was dissected out and incubated for 6 h in a medium containing ³⁵S-methionine and ³⁵S-cysteine. Proteins from the AM were separated by two-dimensional gel electrophoresis, and the gels were exposed to X-ray film. The resulting autofluorographs were analyzed by scanning densitometry. The results show that the incorporation of labeled amino acids was increased in 10 proteins and decreased in 2 proteins in rats killed 17 h after EB. At 42 h after EB, 6 proteins showed an increased incorporation of amino acids and two proteins showed a decrease. Our results suggest that one or several of these proteins might be involved in the neuroendocrine and structural changes observed in the AM during puberty.

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“…The 2-DE procedure of O'Farrell [21) was followed with minor modifications [12,13). In the isoelectric focusing dimension.…”

Section: Polyacrylamide 2-dementioning

confidence: 99%