Effect of remote ischemic postconditioning inflammatory changes in the lung parenchyma of rats submitted to ischemia and reperfusion

Dorsa, Rafael Cantero; Pontes, José Carlos Dorsa Vieira; Antoniolli, Andréia Conceição Brochado; Silva, Guilherme Viotto Rodrigues da; Benfatti, Ricardo Adala; Santos, Carlos Henrique Marques dos; Pontes, Elenir Rose Cury Jardim; Goldiano, José Anderson Souza

doi:10.5935/1678-9741.20150005

Cited by 10 publications

(19 citation statements)

References 11 publications

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“…The authors performed aortic clamping for 30 minutes and reperfusion for 60 minutes, with three cycles of IPC lasting two minutes each. In this study, IPC performed with the same time span used by Dorsa et al [15] did not result in hepatic protection, obtaining results similar to the I/R group. However, there is still a lot of controversy in the literature on the outcome of remote protection of IPC.…”

Section: Discussionsupporting

confidence: 67%

“…This would be true when comparing these two studies evaluating IPC pulmonary action, since there was a difference in the method and this has been the greatest difficulty when comparing the numerous studies of IPC - the wide variation in the method used. The second question refers to the origin of ischemia: Dorsa et al [15] performed aortic clamping while Santos et al [16] and the present study used mesenteric ischemia. This factor may have some influence since in the aortic clamping, the harmful products of the I/R would spread to all the organs and tissues, whereas in the intestinal I/R, all blood is primarily drained to the liver, thus leading to increased toxic concentration of reactive oxygen species on this organ.…”

Section: Discussionmentioning

confidence: 98%

“…In the evaluation of IPC in aortic I/R of rats, Dorsa et al [15] also studied its effectiveness in protecting a distant organ, showing less damage to the lung parenchyma in this group compared to the control group. The authors performed aortic clamping for 30 minutes and reperfusion for 60 minutes, with three cycles of IPC lasting two minutes each.…”

Section: Discussionmentioning

confidence: 99%

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Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Santos¹,

Aydos²,

Neto³

et al. 2016

Brazilian Journal of Cardiovascular Surgery

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IntroductionIschemic postconditioning is a method that shows evidence of efficacy in minimizing reperfusion injury; however, its effectiveness in preventing injuries in distant organs is still unknown, especially in those who have undergone mesenteric ischemia and reperfusion.ObjectiveTo evaluate the effect of ischemic postconditioning in preventing reperfusion injury in the liver of rats submitted to mesenteric ischemia and reperfusion, comparing two different methods of ischemic postconditioning.Methods30 Wistar male rats were used, distributed into three groups: Group A: Ten rats submitted to intestinal ischemia for 30 minutes followed by reperfusion for 60 minutes; Group B: Ten rats subjected to ischemia and reperfusion; after ischemia, two cycles of reperfusion (two minutes each) interleaved with two cycles of ischemia (two minutes each); and Group C: Ten rats subjected to ischemia and reperfusion; after ischemia, four cycles of reperfusion (30 seconds each) interspersed with four cycles of ischemia (30 seconds each). After the experiment, the left lobe of the liver was resected for subsequent histological analysis, using the following classification: grade 1 - centrilobular congestion; grade 2 - centrilobular congestion with some degeneration of hepatocytes in one or two central veins; and grade 3 - multifocal centrilobular congestion and degeneration of portal hepatocytes.ResultsThe mean degree of liver damage found was 1.8 in group A, 1.7 in group B and 1.3 in group C. There was no statistically significant difference between the groups.ConclusionIschemic postconditioning was unable to minimize reperfusion injury in rats undergoing mesenteric ischemia and reperfusion.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Santos¹,

Aydos²,

Neto³

et al. 2016

Brazilian Journal of Cardiovascular Surgery

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“…Recently, Dorsa et al [ 22 ] had demonstrated a protective effect of IPC on lung of rats undergoing aortic ischemia and reperfusion process. They have used also 30 minutes of ischemia and 60 minutes of reperfusion, but it was made by aortic occlusion, differently of our research.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pulmonary reperfusion injury in rats subjected to mesenteric ischemia and reperfusion and protective effect of postconditioning on this process

Santos

Aydos

Neto

et al. 2015

Revista Brasileira De Cirurgia Cardiovascular

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INTRODUCTION Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effectiveness has not been evaluated in the prevention of lung reperfusion injury after mesenteric ischemia and reperfusion.OBJECTIVE To evaluate the presence of pulmonary reperfusion injury and the protective effect of ischemic postconditioning on lung parenchyma in rats submitted to mesenteric ischemia and reperfusion.METHODS Thirty Wistar rats were distributed into three groups: group A (10 rats), which was held mesenteric ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion, interspersed by postconditioning with two alternating cycles of reperfusion and reocclusion, for two minutes each; and group C (10 rats), ischemia and reperfusion interleaved by postconditioning with four alternating cycles of reperfusion and reocclusion of 30 seconds each. Finally, it was resected the upper lung lobe for histological analysis.RESULTS There were mild lung lesions (grade 1) in all samples. There was no statistical difference between groups 1 and 2 (P>0.05).CONCLUSION The mesenteric ischemia and reperfusion in rats for thirty and sixty minutes, respectively, caused mild reperfusion injury in lung. Postconditioning was not able to minimize the remote reperfusion injury and there was no difference comparing two cycles of two minutes with four cycles of 30 seconds.

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“…Santos et al [ 4 ] demonstrated that the efficacy of this method is similar to the efficacy of ischemic preconditioning, and there is no statistically significant difference between the two. This technique is also able to protect tissues at a distance and can be applied in arteries distal to the primary ischemic process, which is effective as demonstrated by Dorsa et al [ 5 ] , and was thus called remote postconditioning.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effects of Atorvastatin and Ischemic Postconditioning Preventing on the Ischemia and Reperfusion Injury: Experimental Study in Rats

Pontes

Neto

et al. 2018

Braz J Cardiovasc Surg

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IntroductionReperfusion injury leads to systemic morphological and functional pathological alterations. Some techniques are already estabilished to attenuate the damage induced by reperfusion. Ischemic preconditioning is one of the standard procedures. In the last 20 years, several experimental trials demonstrated that the ischemic postconditioning presents similar effectiveness. Recently experimental trials demonstrated that statins could be used as pharmacological preconditioning.Methods41 Wistar rats (Rattus norvegicus albinus) were distributed in 5 groups: Ischemia and Reperfusion (A), Ischemic Postconditioning (B), Statin (C), Ischemic Postconditioning + Statins (D) and SHAM (E). After euthanasia, lungs, liver, kidneys and ileum were resected and submitted to histopathological analysis.ResultsThe average of lung parenchymal injury was A=3.6, B=1.6, C=1.2, D=1.2, E=1 (P=0.0029). The average of liver parenchymal injury was A=3, B=1.5, C=1.2, D=1.2, E = 0 (P<0.0001). The average of renal parenchymal injury was A=4, B=2.44, C=1.22, D=1.11, E=1 (P<0.0001). The average of intestinal parenchymal injury was A=2, B=0.66, C=0, D=0, E=0 (P=0.0006). The results were submitted to statistics applying Kruskal-Wallis test, estabilishing level of significance P<0.05.ConclusionGroups submitted to ischemic postconditioning, to pre-treatment with statins and both methods associated demonstrated less remote reperfusion injuries, compared to the group submitted to ischemia and reperfusion without protection.

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Effect of remote ischemic postconditioning inflammatory changes in the lung parenchyma of rats submitted to ischemia and reperfusion

Cited by 10 publications

References 11 publications

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Evaluation of pulmonary reperfusion injury in rats subjected to mesenteric ischemia and reperfusion and protective effect of postconditioning on this process

Evaluation of the Effects of Atorvastatin and Ischemic Postconditioning Preventing on the Ischemia and Reperfusion Injury: Experimental Study in Rats

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