Effect of RNA interference-related HiWi gene expression on the proliferation and apoptosis of lung cancer stem cells

Fang, Zhenhao; Dong, Min; Liang, Chao; Xing, Chonghao; Zhao, Juan; Yang, Yi-Ju

doi:10.3892/ol.2012.677

Cited by 28 publications

(29 citation statements)

References 10 publications

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“…In addition, it was reported that the overexpression of p21 resulted in increased Bax expression and the induction of apoptosis. liang et al demonstrated that silencing HiWi induced apoptosis in lung cancer stem cells (13). In this study, data showed that silencing HiWi also stimulated u251 and a172 apoptosis, increased Bax expression, and decreased Bcl-2 expression.…”

Section: Discussionsupporting

confidence: 51%

“…Previous studies revealed that cell proliferation is inhibited following the silencing of HiWi in human gastric cancer and lung cancer tumor stem cells (13,22). This was due to inducing cell cycle arrest at G2/M phase and inducing apoptosis, respectively, after the suppression of HiWi expression.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated that HiWi is expressed in tumor tissues, and is one of the most important elements in the regulation of cancer cells (8,9). HIWI was also correlated with patient prognosis in pancreatic, colon, colorectal, lung, and endometrial cancer (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

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Silencing HIWI suppresses the growth, invasion and migration of glioma cells

Wang

Tong

Gao

et al. 2014

International Journal of Oncology

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Abstract. the HiWi gene is one of the members of the PiWi gene family that is important for stem cell self-renewal and expressed highly in certain human tumors. Some studies have demonstrated that HiWi plays a key role in the development of tumors in cervical, colon and liver cancer. Previous studies have demonstrated that HiWi is associated with prognosis of patients with glioma. However, there is no report on the analysis of HiWi in the biological characteristics of glioma cells. the aim of the study was to investigate whether HiWi plays an important role in the progress of glioma. Silencing HiWi inhibited cell proliferation by promoting apoptosis and increased cell cycle arrest. the expression of proteins related to apoptosis and the cell cycle, including p21, cyclin D1, Bcl-2, and Bax was significantly altered. Moreover, knockdown of HiWi inhibited the migration and invasion of glioma cells by reducing the expression of MMP-2 and MMP-9. furthermore, we found that reduction of HiWi inhibited tumor growth in vivo. These findings suggest that HIWI is an oncogene involved in the progression of glioma.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

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Silencing HIWI suppresses the growth, invasion and migration of glioma cells

Wang

Tong

Gao

et al. 2014

International Journal of Oncology

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“…Furthermore, HIWI has been shown to be highly expressed in numerous human cancer types (11,15), and abnormal HIWI expression levels have been associated with a poor prognosis in various human malignant tumors, including human esophageal squamous cell carcinoma (16), human adenocarcinoma of the pancreas (15), human gastric cancer (11), and colorectal cancer (17). In addition, a previous study demonstrated that silencing of the HIWI gene was able to decrease the proliferation and promote the apoptosis of lung cancer tumor stem cells (18), and it was suggested that the hiwi gene may be a key regulator in the maintenance of cancer stem cell (CSC) populations in lung cancers (19,20 HIWI has been implicated in the tumorigenesis of lung cancers and may be a potential target for novel cancer therapies. The present study aimed to investigate whether HIWI is overexpressed in NSCLC specimens, and the effects of manipulating the expression levels of HIWI on the proliferation of a human A549 NSCLC cell line.…”

Section: Introductionmentioning

confidence: 99%

Manipulations in HIWI level exerts influence on the proliferation of human non-small cell lung cancer cells

Wang

Liu

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Lung cancer is the leading cause of cancer-associated mortality worldwide, although molecular imaging techniques, including fludeoxyglucose positron emission tomography, have markedly improved the diagnosis of lung cancer. HIWI is a member of the human piwi family, members of which are known for their roles in RNA silencing. HIWI has been shown to serve a crucial function in stem cell self-renewal, and previous studies have reported HIWI overexpression in lung cancers. Furthermore, HIWI has been proposed to regulate the maintenance of cancer stem cell populations in lung cancers. The present study investigated the mRNA and protein expression levels of HIWI in non-small cell lung cancer (NSCLC) specimens harvested from 57 patients, using reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Subsequently, the HIWI expression level was manipulated using gain-of-function and loss-of-function strategies, and the role of HIWI in the proliferation of human A549 NSCLC cells was investigated using Cell Counting Kit-8 and colony formation assays. The mRNA and protein expression levels of HIWI were significantly upregulated in the intratumor NSCLC specimens, as compared with the peritumor specimens. Furthermore, the mRNA and protein expression levels of HIWI in A549 cells were successfully manipulated using the two strategies. Overexpression and knockout of HIWI were associated with the promotion and inhibition of A549 cell proliferation, respectively. The results of the present study suggested that HIWI is overexpressed in NSCLC tissues and demonstrated that upregulation of HIWI may promote the growth of lung cancer cells; thus suggesting that HIWI may have an oncogenic role in lung cancer. IntroductionLung cancer is the leading cause of cancer-associated mortality worldwide (1), among which non-small cell lung cancer (NSCLC) accounts for ~80% of all cases of lung cancer (2). The incidence of NSCLC in China has markedly increased over the past 5 years (3). At present, numerous clinical and molecular prognostic factors are considered in order to tailor therapies to particular subgroups of patients; however, treatment responses are routinely assessed by serial measurements of tumour sizes prior to and following treatment (4). Molecular imaging, which is a research and clinical tool that was developed >30 years ago (5), has markedly improved the diagnosis and the treatment of lung cancers. Previous studies have suggested the superior prognostic value of fludeoxyglucose positron emission tomography, as compared with computed tomography, and it has exhibited enhanced sensitivity for the evaluation of therapeutic effects (6-8). However, NSCLC is typically diagnosed at the advanced stages, leading to a poor prognosis in China (9).The hiwi gene, which is a human homologue of the piwi family, is located at 12q24.33 and encodes the 98.5 kDa HIWI protein (10). The piwi family has been shown to have an important role in stem cell self-renewal in diverse organisms, including je...

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“…Note HIWI knockdown decreased cell proliferation and promoted apoptosis of lung cancer stem cells (Liang et al, 2013;Liang et al, 2012).…”

Section: Lung Cancermentioning

confidence: 99%