Effect of rumen-protected branched-chain amino acid supplementation on production- and energy-related metabolites during the first 35 days in milk in Holstein dairy cows

Yepes, F.A. Leal; Mann, Sabine; Overton, T.R.; Ryan, C. W.; Bristol, L.S.; Granados, G.E.; Nydam, D.V.; Wakshlag, Joseph J.

doi:10.3168/jds.2018-15508

Cited by 20 publications

(20 citation statements)

References 81 publications

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“…Furthermore, feeding BCAA increased milk protein yield and content when supplemented in combination with Met and Lys (Appuhamy et al, 2012). Dietary supplementation with rumen-protected BCAA also decreased the incidence of hyperketonemia (Yepes et al, 2019) and the severity of hepatic lipidosis during early lactation (Leal Yepes et al, 2021). These findings suggest that greater BCAA availability can positively affect various metabolic processes.…”

Section: Effects Of Dietary Branched-chain Amino Acid Supplementation...mentioning

confidence: 81%

“…Accordingly, the mammary uptake of BCAA was shown to be greater than the milk output (Lapierre et al, 2012), indicating the catabolism of BCAA in the mammary gland. In addition, dietary BCAA supplementation improved their concentrations in serum (Yepes et al, 2019). Furthermore, feeding BCAA increased milk protein yield and content when supplemented in combination with Met and Lys (Appuhamy et al, 2012).…”

Section: Effects Of Dietary Branched-chain Amino Acid Supplementation...mentioning

confidence: 93%

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Effects of dietary branched-chain amino acid supplementation on serum and milk metabolome profiles in dairy cows during early lactation

Kenéz

Mann

et al. 2022

Journal of Dairy Science

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The 3 branched-chain AA (BCAA), Val, Leu, and Ile, are essential AA used by tissues as substrates for protein synthesis and energy generation. In addition, BCAA are also involved in modulating cell signaling pathways, such as nutrient sensing and insulin signaling. In our previous study, dietary BCAA supplementation was shown to improve protein synthesis and glucose homeostasis in transition cows. However, a more detailed understanding of the changes in metabolic pathways associated with an increased BCAA availability is desired to fine-tune nutritional supplementation strategies. Multiparous Holstein cows (n = 20) were enrolled 28 d before expected calving and assigned to either the BCAA treatment (n = 10) or the control group (n = 10). Cows assigned to BCAA were fed 550 g/d of rumenprotected BCAA mixed with 200 g/d of dry molasses from calving until 35 DIM, whereas the cows assigned to the control were fed only 200 g/d of dry molasses. Serum samples were collected on d 10 before expected calving, as well as on d 4 and d 21 postpartum. Milk samples were collected on d 14 postpartum. From a larger cohort, we selected 20 BCAA-supplemented cows with the greatest plasma urea nitrogen concentration, as an indicator for greater BCAA availability, for the metabolomics analysis herein. Serum and milk samples were subjected to a liquid chromatography-mass spectrometry-based assay, detecting and measuring the abundance of 241 serum and 211 milk metabolic features, respectively. Multivariable statistical analyses revealed that BCAA supplementation altered the metabolome profiles of both serum and milk samples. Increased abundance of serum phosphocholine and glutathione and of milk Val, Ile, and Leu, and decreased abundance of milk acyl-carnitines were associated with BCAA supplementation. Altered phosphocholine and glutathione abundances point to altered hepatic choline metabolism and antioxidant balance, respectively. Altered milk acyl-carnitine abundances suggest changes in mammary fatty acid metabolism. Dietary BCAA supplementation was associated with a range of alterations in serum and milk metabolome profiles, adding to our understanding of the role of BCAA availability in modulating dairy cow protein, lipid, and energy metabolism on a whole-body level and how it affects milk composition.

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Section: Effects Of Dietary Branched-chain Amino Acid Supplementation...mentioning

confidence: 81%

Section: Effects Of Dietary Branched-chain Amino Acid Supplementation...mentioning

confidence: 93%

Effects of dietary branched-chain amino acid supplementation on serum and milk metabolome profiles in dairy cows during early lactation

Kenéz

Mann

et al. 2022

Journal of Dairy Science

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“…Branched-chain AA (BCAA) supplementation has been found to be beneficial for body weight, lipogenesis, and insulin resistance in several species [ 69 , 70 , 71 ]. BCAAs are also known to improve milk and body protein synthesis and get oxidized by the tricarboxylic acid cycle to produce ATP during catabolic states [ 72 ]. In the HPF group, the enrichment of the tricarboxylic acid cycle suggests an increase in the mutual conversion of body fat, sugar, and protein and the increased synthesis of microbial protein.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Potential Role of the Rumen Microbiome in Milk Protein and Fat Synthesis in Dairy Cows Using Metagenomic Sequencing

Huang

et al. 2021

Animals

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The rumen contains abundant microorganisms that aid in the digestion of lignocellulosic feed and are associated with host phenotype traits. Cows with extremely high milk protein and fat percentages (HPF; n = 3) and low milk protein and fat percentages (LPF; n = 3) were selected from 4000 lactating Holstein cows under the same nutritional and management conditions. We found that the total concentration of volatile fatty acids, acetate, butyrate, and propionate in the rumen fluid was significantly higher in the HPF group than in the LPF group. Moreover, we identified 38 most abundant species displaying differential richness between the two groups, in which Prevotella accounted for 68.8% of the species, with the highest abundance in the HPF group. Functional annotation based on the Kyoto Encyclopedia of Gene and Genome (KEGG), evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG), and Carbohydrate-Active enzymes (CAZy) databases showed that the significantly more abundant species in the HPF group are enriched in carbohydrate, amino acid, pyruvate, insulin, and lipid metabolism and transportation. Furthermore, Spearman’s rank correlation analysis revealed that specific microbial taxa (mainly the Prevotella species and Neocallimastix californiae) are positively correlated with total volatile fatty acids (VFA). Collectively, we found that the HPF group was enriched with several Prevotella species related to the total VFA, acetate, and amino acid synthesis. Thereby, these fulfilled the host’s needs for energy, fat, and rumen microbial protein, which can be used for increased biosynthesis of milk fat and milk protein. Our findings provide novel information for elucidation of the regulatory mechanism of the rumen in the formation of milk composition.

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“…Branched-chain amino acids may be used for synthesis of muscle or milk protein, preserved into the cell for structural protein synthesis, used as precursor for different metabolic and catabolic processes, or passes unaltered into milk, blood, or lymph [47]. The greater branched-chain amino acid concentrations observed in response to feeding CTR than ORG could be indicative of greater utilization by other peripheral tissues such as adipose [48]. Additionally, the lack of differences for BOHB or NEFA suggested little effect of diets in whole-body energy balance [49].…”

Section: Discussionmentioning

confidence: 99%

Alterations in Energy Partitioning and Methane Emissions in Murciano-Granadina Goats Fed Orange Leaves and Rice Straw as a Replacement for Beet Pulp and Barley Straw

Romero

Palomares

Moya

et al. 2020

Animals

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Considering the huge quantities of crops by-products and pruning waste such as rice straw and citrus leaves produced annually worldwide, and their potential pollution capacity, recycling as feed for livestock is an alternative. The objective was to study these by-products effect on energy balance and methane emissions in 10 Murciano-Granadina goats at maintenance. The control diet (CTR) included barley straw and beet pulp while the experimental diet (ORG) consisted of rice straw and orange leaves. Differences were found for energy intake (248 kJ/kg of BW0.75 greater for CTR than ORG). The intake of metabolizable energy was 199 kJ/kg of BW0.75 lower in ORG than CTR, and the energy efficiency was higher with CTR (0.61) than ORG (0.48). Protein retained in the body was 9 g/goat greater with CTR than ORG, and fat retention in the body was approximately 108 g/goat greater with CTR than ORG. Despite more unfavorable energy balance in response to feeding ORG than CTR, the retention of body energy was always positive. Reductions in CH4 emissions were detected when goats were fed ORG diet (from 22.3 to 20.0 g/d). Overall results suggested that feeding orange leaves and rice straw was effective in reducing CH4 emissions without adversely affecting energy balance.

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Effect of rumen-protected branched-chain amino acid supplementation on production- and energy-related metabolites during the first 35 days in milk in Holstein dairy cows

Cited by 20 publications

References 81 publications

Effects of dietary branched-chain amino acid supplementation on serum and milk metabolome profiles in dairy cows during early lactation

Effects of dietary branched-chain amino acid supplementation on serum and milk metabolome profiles in dairy cows during early lactation

Identification of the Potential Role of the Rumen Microbiome in Milk Protein and Fat Synthesis in Dairy Cows Using Metagenomic Sequencing

Alterations in Energy Partitioning and Methane Emissions in Murciano-Granadina Goats Fed Orange Leaves and Rice Straw as a Replacement for Beet Pulp and Barley Straw

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