Effect of saccharides on secondary compounds production from stem derived callus of Datura inoxia

Twaij, Baan Munim; Taha, Alaa Jabbar; Bhuiyan, Md. Fahmid Hossain; Hasan, Nahid

doi:10.1016/j.btre.2022.e00701

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Saccharides are the major bioactive nutrients in food materials or products, including monosaccharides, disaccharides, oligosaccharides, and polysaccharides. They are the most important elicitors to alter physiological and biochemical response in plants, and influence secondary compound productions [ 29 ]. Recently, oligosaccharides and polysaccharides obtained from A. lappa have received extensive attention, and inulin-type fructan is the major component.…”

Section: Resultsmentioning

confidence: 99%

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Qiu

Jiang

et al. 2022

Foods

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This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in Arctium lappa L. (A. lappa) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in A. lappa roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in A. lappa roots, which would promote the processing technologies for A. lappa roots and help us to understand the plant metabolism process.

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Section: Resultsmentioning

confidence: 99%

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Qiu

Jiang

et al. 2022

Foods

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“…Different compounds present in these elicitors have been implicated in plant defense responses, including the activation of signaling pathways, ethylene production, and inhibition of auxin-induced responses [ 15 , 16 ]. It has been used successfully in both organized and unorganized cultures of plants to increase the production of miscellaneous bioactive molecules [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Kanthaliya

Joshi

Arora

et al. 2023

Plants

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Pueraria tuberosa contains a wide range of bioactive compounds, including polyphenols, alkaloids, and phytosterols, which make it valuable to the pharmaceutical and food industries. Elicitor compounds trigger the defense mechanisms in plants and are widely used to increase the yield of bioactive molecules in in vitro cultures. The present study was conducted to evaluate the effects of different concentrations of biotic elicitors such as yeast extract (YE), pectin (PEC), and alginate (ALG) on growth, antioxidant activity, and metabolite accumulation in in vitro propagated shoots of P. tuberosa. The elicitors applied to shoot cultures of P. tuberosa significantly increased biomass (shoot number, fresh weight, and dry weight), and metabolites such as protein, carbohydrates, chlorophyll, total phenol (TP), and total flavonoid (TF) contents, as well as antioxidant activity compared to untreated control. Biomass, TP, and TF contents, as well as antioxidant activity, were most significant in cultures treated with 100 mg/L PEC. In contrast, chlorophyll, protein, and carbohydrate increased most in cultures treated with 200 mg/L ALG. Application of 100 mg/L of PEC led to the accumulation of high amounts of isoflavonoids including puerarin (220.69 μg/g), daidzin (2935.55 μg/g), genistin (5612 μg/g), daidzein (479.81 μg/g), and biochanin-A (111.511 μg/g) as analyzed by high-performance liquid chromatography (HPLC). Total isoflavonoids content of 100 mg/L PEC treated shoots was obtained as 9359.56 μg/g, 1.68-fold higher than in vitro propagated shoots without elicitors (5573.13 μg/g) and 2.77-fold higher than shoots of the mother plant (3380.17 μg/g). The elicitor concentrations were optimized as 200 mg/L YE, 100 mg/L PEC, and 200 mg/L ALG. Overall, this study showed that the application of different biotic elicitors resulted in better growth, antioxidant activity, and accumulation of metabolites in P. tuberosa, which could lead to obtaining phytopharmaceutical advantages in the future.

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“…It has been well-documented that plant species belonging to the Datura genus possess a variety of secondary metabolites, such as alkaloids, terpenoids, and avonoids, which have valuable medicinal and therapeutic applications [9,10]. For this reason, various strategies, such as applications of chemical [11], physical [10], and biotic elicitors [12] have been employed to stimulate the production of secondary metabolites of this plant under laboratory cultivation conditions.…”

Section: Introductionmentioning

confidence: 99%

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in-vitro cultures

Tardast,

Iranbakhsh,

Ebadi

et al. 2023

Preprint

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This study aims to monitor growth, biochemical, and anatomical responses of Datura seeds to cold plasma treatments for 0, 60, 120, 180 and 300 seconds. The plasma treatments of 60, 120 and 180 s improved the growth performance of seedlings, while the plasma treatment of 300 s drastically declined growth performance. The most pronounced response to the plasma treatments was on root growth and biomass. The proline concentrations in both root and leaves displayed a linear significant upward trend in response to the plasma treatments. The plasma treatment for 180s was the most effective method to increase tropane alkaloids in leaves about 2-fold. With increasing the plasma duration time from 60 to 300 s, the soluble phenols in leaves were linearly enhanced. The P60, P120, and P180 treatments augmented total protein concentrations by an average of 32.9%, while the P300 treatments significantly diminished protein content by 27.2%. The applied treatments also enhanced the soluble sugars in leaves by an average of 48%. The highest amounts of photosynthetic pigments (chlorophylls and carotenoids) were recorded in the P120 and P180 treatment groups contrasted with P300. The activities of two enzymatic antioxidants, including catalase and peroxidase, showed a similar upward trend to that of proline. The plasma priming also improved the phenylalanine ammonia lyase (PAL) activity (a secondary metabolism index) by an average of 65.6%. Further investigations are needed to optimize plasma treatment parameters and understand the underlying mechanisms to maximize its benefits while minimizing potential risks.

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Effect of saccharides on secondary compounds production from stem derived callus of Datura inoxia

Cited by 5 publications

References 15 publications

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in-vitro cultures

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