Effect of salicylic acid (SA) seeds soaking on the NaCl salt stress induced changes in soluble sugar and protein accumulation in organs of two genotypes of okra plants

Abstract

Salt stress is a major challenge in agricultural system. In this study, okra seeds of two genotypes (47-7 and LD 88) were presoaked with 10 -2 , 10 -4, and 10 -6 mM salicylic acid and control in distilled water, then the soil was treated with 0, 50, 100, 150 and 200 mM NaCl. The experiment was conducted to study the effect on osmoregulating solutes such as proline, salt stress protein (glycine betaine and proline betaine) and soluble sugars (glucose and fructose). Results showed that proline content increased with increased in the concentrations of salinity. Also, treatment with salicylic acid (SA) improved salt stress proteins accumulation in both stressed genotypes. In contrast, decreased SA concentrations improved soluble sugar accumulation in the fruit of okra genotype 47-4. But in LD88, increased in the level of SA resulted to the increased soluble sugar accumulation in the leaf. Combined effect of SA and salinity caused a greater accumulation of protein and soluble sugar in leaf and fruit of both genotypes of stressed okra, but significant increased were seen only in the groups of LD88 treated with 10 -4 mM SA at 50 mM NaCl in leaf and 10 -2 mM SA at 150 mM NaCl in fruit when compared with the control group. Salinity induced a marked decreased in reducing sugar accumulation of okra plant (LD88), especially at high salinity level (200 mM NaCl). Therefore, accumulations of compatible solutes such as salt stress proteins may provide plant a storage form of nitrogen that will be re-utilized later and may play a role in osmotic adjustment.

Timing of salicylic acid application affects the response of maize (Zea mays L.) hybrids to salinity stress

“…Exogenous application of SA has been reported to mitigate the adverse effect of salinity on maize growth by increasing production of sugars (Fahad and Bano, 2012) and proline (Hussein et al., 2007). The contribution of proline but not soluble sugars (Esan and Olaiya, 2016) and of proline and Na + but not K + in osmoregulation of okra and maize, respectively under salinity stress has been reported by Cicek and Cakirlar (2002). The limited alterations in protein and phenolics contents of maize leaves in response to hybrid, salinity and SA regime point to limited genotypic variability in these two components and suggests that neither protein nor phenolics can be considered as markers for salt injury/resistance in the two used maize hybrids.…”

Prohexadione Calcium and Gibberellin Improve Osmoregulation, Antioxidant Response and Ion Homeostasis to Alleviate NaCl Stress in Rice Seedlings

“…In this study, Guang Hong 3 accumulated more proline and soluble protein than the Huang Huazhan variety. It was previously claimed that high concentrations of proline enhance plant tolerance to NaCl stress by increasing the osmoregulatory capacity of plants and reducing cell damage by ROS [37,38]. Proline accumulation is a criterion for determining plant tolerance to salt stress [39].…”

Effect of Salicylic Acid on Biochemical and Physiological Parameters of Two Genotypes of Okra under Salinity Stress

“…Various studies indicate the ability of exogenous application of SA under saline conditions to increase the accu-International Journal of Food Science and Agriculture mulation of osmolytes, enhance antioxidant protection, and maintain optimum Na + /K + ratio, which is suggested as potential mechanisms of salt tolerance in plants [11][12]. Our previous works indicated that SA minimizes the negative effects of salt stress by improving accumulation of compatible solutes, and increasing antioxidant activity [13][14]. The aim of this study was to investigate the impact of salicylic acid on some key biochemical and physiological parameters in salt-stressed okra.…”