Effect of salinity fluctuation on the transcriptome of the Japanese mantis shrimp Oratosquilla oratoria

Lou, Fangrui; Gao, Tianxiang; Han, Zhiqiang

doi:10.1016/j.ijbiomac.2019.08.223

Cited by 20 publications

(13 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have showed that Na + and Cl − account for a relatively major proportion of the composition of haemolymph in most marine organisms [ 29 ]. Our results showed that several LSST-related SNPs are located in ion transport-related genes ( SLC6A5 ; sodium- and chloride-dependent glycine transporter 2), and these genes may influence the sensitivity of different C. lucidus populations to Na + and Cl − [ 30 ]. In fact, the salinity of the low-temperature areas offshore China is higher than that of the high-temperature areas, therefore, we suspected that different C. lucidus populations have evolved different osmotic pressure regulation abilities.…”

Section: Discussionmentioning

confidence: 99%

Population genomics provides insights into the population structure and temperature-driven adaptation of Collichthys lucidus

Zhao

Song

et al. 2021

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background Understanding the genetic structure and local adaptive evolutionary mechanisms of marine organisms is crucial for the management of biological resources. As the ecologically and commercially important small-sized shallow-sea fish, Collichthys lucidus plays a vital role in the structure and functioning of marine ecosystem processes. C. lucidus has been shown to have an obvious population structure. Therefore, it is an ideal candidate for investigating population differentiation and local adaptation under heterogeneous environmental pressure. Results A total of 184,708 high-quality single nucleotide polymorphisms (SNPs) were identified and applied to elucidate the fine-scale genetic structure and local thermal adaptation of 8 C. lucidus populations. Population structure analysis based on all SNPs indicated that the northern group and southern group of C. lucidus have a strong differentiation. Moreover, 314 SNPs were found to be significantly associated with temperature variation, and annotations of genes containing temperature-related SNPs suggested that genes were involved in material (protein, lipid, and carbohydrate) metabolism and immune responses. Conclusion The high genetic differentiation of 8 C. lucidus populations may have been caused by long-term geographic isolation during the glacial period. Moreover, we suspected that variation in these genes associated with material (protein, lipid, and carbohydrate) metabolism and immune responses was critical for adaptation to spatially heterogeneous temperatures in natural C. lucidus populations. In conclusion, this study could help us determine how C. lucidus populations will respond to future ocean temperature rising.

show abstract

Section: Discussionmentioning

confidence: 99%

Population genomics provides insights into the population structure and temperature-driven adaptation of Collichthys lucidus

Zhao

Song

et al. 2021

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to its direct damaging effects on many tissues and organs of aquatic animals (Li et al, 2015), ammonia toxicity or stress also results in oxidative damage and increase in the levels of free reactive oxygen species (ROS) in shrimp (Ching et al, 2009; Zhang et al, 2015b). Changes in environmental factors, such as salinity, pH and temperature, give rise to fluctuations in ammonia concentration in water, which decreases the feeding rate, growth rate and immune function of aquatic organisms as well as predispose them to diseases (Cui et al, 2017; Han et al, 2018; Qiu et al, 2018; Lou et al, 2019). For instance, when ammonia levels in aquaculture water reach a certain mass concentration, non‐ionic ammonia could easily enter shrimp body via cell membranes, resulting in physiological imbalance and inhibition of growth (Naqvi et al, 2007; Xiao et al, 2019).…”

Section: Effect Of Ammonia Levels On Shrimp Growth and Survivalmentioning

confidence: 99%

“…Unfortunately, the rapid growth and expansion in shrimp farming have also resulted in degradation and pollution of the aquaculture fields and surrounding environment. For instance, a growing number of studies have shown aquaculture fields and ponds to have undergone drastic changes in salinity, pH, temperature, ammonia, etc., which impact on aquatic organisms and their immune response (Xu et al, 2018; Han et al, 2018; Qiu et al, 2018; Lou et al, 2019). The most noxious among these parameters is ammonia, which is reported to affect numerous marine species (see Table 1).…”

Section: Introductionmentioning

confidence: 99%

Effects of ammonia on shrimp physiology and immunity: a review

Zhao

Yao

et al. 2020

Reviews in Aquaculture

103

View full text Add to dashboard Cite

The rapid expansion in shrimp farming comes with problems such as inadequate culture technology, disease outbreak, water pollution and other environmental degradation‐related problems. Prominent among these is the level of ammonia, which has been rising steadily in the last couple of years, mainly due to farming practices and other anthropogenic activities, and therefore has had consequent effects on marine life, especially shrimp. While optimal ammonia levels are a good source of nitrogen for marine phytoplankton that increases the level of dissolved oxygen in water as well serves as food for shrimp, high levels of ammonia are harmful to shrimp. When shrimp pond ammonia levels go beyond the tolerance limits, it inhibits chitinase expression, moulting, growth, phenoloxidase and haemolymph antimicrobial activity, thereby attenuating shrimp innate immune response. In this review, we bring together recent information on the effects of ammonia stress on the growth, physiology, biochemistry, ammonia‐metabolizing enzymes and immunity of shrimp. We also propose areas of research that can be explored in the breeding of new ammonia‐tolerant and disease‐resistant shrimp with robust immune and/or physiological systems that could withstand environmental stress and pathogens.

show abstract

“…The acquisition of transcriptome will help to explore the gene-level regulation mechanisms in C. japonica under salinity fluctuation (Wang et al, 2009), but relevant studies have yet to be developed. At present, RNA sequencing (RNA-seq) based on highthroughput sequencing has been successfully used to identify gene expression patterns in a variety of crustaceans under salinity stresses, such as Oratosquilla oratoria (Lou et al, 2019), Scylla paramamosain (Wang et al, 2018) and others. Meanwhile, salinity fluctuations have also been shown to activate some gene (i.e.…”

Section: Introductionmentioning

confidence: 99%

Salinity Fluctuation on the Genetic Regulatory Mechanisms of the Crustacean, Charybdis japonica

et al. 2022

Self Cite

View full text Add to dashboard Cite

The intertidal salinity fluctuations provide pose a significant physical challenge for the crustaceans that live here. In the present study, Charybdis japonica was selected as the research species to explore the genetic regulatory mechanisms of intertidal crustaceans responding to salinity fluctuations. We cultured C. japonica s with three salinity gradients (the salinity of 15‰, 25‰, and 35‰) and 68.90 Gb clean transcriptome reads were obtained by RNA-seq. All clean reads were then de novo assembled to 41,058 unigene with a mean length of 1,179.39 bp and an N50 length of 2,033 bp. Furthermore, a total of 18,100 (24.74%) unigenes were successfully matched with the sequences from at least one database. We further analyzed the transcriptome structure of C. japonica and a total of 26,853 CDSs and 12,190 SSRs were predicted. The gene expression levels of C. japonica at 25‰ were used as control, and 204 and 5,392 DEGs were found at the salinity of 35‰ and 15‰, respectively. Based on the annotated information of DEGs, we speculated that C. japonicas were subjected to greater stress under the salinity of 35‰ and isotonic intracellular regulation was activated. Furthermore, neuronal activity, oxidation response, intracellular regulatory activity and osmotic regulation-related genes were speculated to be critical genes for C. japonica to cope with salinity fluctuations. The present results will provide fundamental information for revealing the salinity fluctuation-related genetic regulatory mechanisms of C. japonica and other intertidal crustaceans, and then help us predict how future salinity changes will affect the survival of crustaceans.

show abstract

Effect of salinity fluctuation on the transcriptome of the Japanese mantis shrimp Oratosquilla oratoria

Cited by 20 publications

References 61 publications

Population genomics provides insights into the population structure and temperature-driven adaptation of Collichthys lucidus

Population genomics provides insights into the population structure and temperature-driven adaptation of Collichthys lucidus

Effects of ammonia on shrimp physiology and immunity: a review

Salinity Fluctuation on the Genetic Regulatory Mechanisms of the Crustacean, Charybdis japonica

Contact Info

Product

Resources

About