Effect of salt type and concentration on the growth and lipid content of Chlorella vulgaris in synthetic saline wastewater for biofuel production

Church, Jared; Hwang, Jae-Hoon; Kim, Keugtae; McLean, Rebecca; Oh, You-Kwan; Nam, Bora; Joo, Jin Chul; Lee, Woo Hyoung

doi:10.1016/j.biortech.2017.06.081

Cited by 131 publications

(40 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, salinity of FW (2.25%) was close to the inhibitive level of 2.5% for the growth of C. sorokiniana ; and turbidity of FW (59.1 NTU) was significantly higher than the reported optimal turbidity (less than 10 NTU) for microalgae growth . As reported by Church et al ., the specific growth rate of C. vulgaris decreased with the increase of salinity, suggesting that salinity of the culture medium would affect the algal growth. Moreover, it was reported that turbidity of the medium would limit the light availability for algae photosynthesis because it could impede light penetration necessary for algal growth .…”

Section: Resultssupporting

confidence: 60%

Cultivation of Chlorella sorokiniana using wastewaters from different processing units of the silk industry for enhancing biomass production and nutrient removal

Deng

Xue

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Previous studies have documented that searching for wastewaters with appropriate nutrient profiles to cultivate microalgae directly was an effective measure to reduce the cost of algae cultivation. In this work, wastewaters collected from different processing units of the silk industry were used to cultivate Chlorella sorokiniana directly or after preliminary pretreatment for producing algal biomass and removing nutrients. RESULTS:The data showed that C. sorokiniana could grow on reeling wastewater and cooking cocoon wastewater with a maximum biomass of 0.26 and 0.49 g L −1 , respectively, but could not grow on frigon wastewater because of high salinity and turbidity. Furthermore, the binary mixed wastewaters were used to cultivate this alga, which were mixed according to the algal nutritional requirements. Results indicated that the highest algal biomass (0.56 g L −1 ), and total nitrogen and phosphorus removal efficiencies (71.7% and 97.1%) were obtained when frigon wastewater was diluted with reeling wastewater (v/v) at a dilution multiple of eight, which was used as the algal culture media. Additionally, contents of protein, lipids, carbohydrates, and pigments in the algal cells varied with the changes in nutrient profiles of wastewaters. CONCLUSIONS: It is concluded that (i) reeling wastewater and cooking cocoon wastewater were rich in nutrients for the growth of C. sorokiniana, which could be used as algal culture medium directly; and (ii) growing C. sorokiniana on mixed wastewaters could enhance biomass production and nutrient removal. Thus, the silk industry wastewaters could be considered as suitable media to cultivate microalgae directly or by simply mixing. Figure 4. Variations in the concentrations of nutrients and the culture's pH when Chlorella sorokiniana grew on the mixed silk industry wastewater (SIW) for 7 days. (a) Total nitorgen (TN) removal; (b) total phosphorus (TP) removal; (c) chemical oxygen demand (COD) removal; and (d) pH variation. Each point represents the mean of three replicates, and the error bars represent the standard deviation.wileyonlinelibrary.com/jctb

show abstract

Section: Resultssupporting

confidence: 60%

Cultivation of Chlorella sorokiniana using wastewaters from different processing units of the silk industry for enhancing biomass production and nutrient removal

Deng

Xue

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…1d). The saltcaused growth impairment to different degrees has been reported for many other freshwater algal strains [25][26][27][28][29][31][32][33][34][35]. The content of total fatty acids (TFA) showed a considerable increase (over twofold) under the moderate salt concentration of 0.2 M, but did not increase further under high salt concentrations ( Fig.…”

Section: Optimization Of Salinity Levels For Lipid and Astaxanthin Prmentioning

confidence: 57%

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Mao

Zhang

Wang

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background: Chromochloris zofingiensis, a freshwater alga capable of synthesizing both triacylglycerol (TAG) and astaxanthin, has been receiving increasing attention as a leading candidate producer. While the mechanism of oleaginousness and/or carotenogenesis has been studied under such induction conditions as nitrogen deprivation, high light and glucose feeding, it remains to be elucidated in response to salt stress, a condition critical for reducing freshwater footprint during algal production processes. Results:Firstly, the effect of salt concentrations on growth, lipids and carotenoids was examined for C. zofingiensis, and 0.2 M NaCl demonstrated to be the optimal salt concentration for maximizing both TAG and astaxanthin production. Then, the time-resolved lipid and carotenoid profiles and comparative transcriptomes and metabolomes were generated in response to the optimized salt concentration for congruent analysis. A global response was triggered in C. zofingiensis allowing acclimation to salt stress, including photosynthesis impairment, ROS build-up, protein turnover, starch degradation, and TAG and astaxanthin accumulation. The lipid metabolism involved a set of stimulated biological pathways that contributed to carbon precursors, energy and reductant molecules, pushing and pulling power, and storage sink for TAG accumulation. On the other hand, salt stress suppressed lutein biosynthesis, stimulated astaxanthin biosynthesis (mainly via ketolation), yet had little effect on total carotenoid flux, leading to astaxanthin accumulation at the expense of lutein. Astaxanthin was predominantly esterified and accumulated in a well-coordinated manner with TAG, pointing to the presence of common regulators and potential communication for the two compounds. Furthermore, the comparison between salt stress and nitrogen deprivation conditions revealed distinctions in TAG and astaxanthin biosynthesis as well as critical genes with engineering potential. Conclusions:Our multi-omics data and integrated analysis shed light on the salt acclimation of C. zofingiensis and underlying mechanisms of TAG and astaxanthin biosynthesis, provide engineering implications into future trait improvements, and will benefit the development of this alga for production uses under saline environment, thus reducing the footprint of freshwater.

show abstract

“…Scenedesmus Obliquus algae is used for saline water desalination by several researchers with successful results to produce fresh water with retention time varied between 7 -21 days according to inlet salinity [4,5,6,7]. Chlorella Vulgaris was found to withstand high levels of salt concentration which is a [8]. However this is a fortunate finding as it is used by this study for electricity production and bio desalination under high salt conditions.…”

Section: Introductionmentioning

confidence: 83%

Electricity Production from Desalination by Algal Ponds using Chlorella Vulgaris

L.*,

H.,

et al. 2019

IJEAT

View full text Add to dashboard Cite

The demand for natural resources is rising tremendously as a result of the current urban expansion. Water and energy are the two main focuses of the environmental scientific society due to their anticipated scarcity. A desalination approach using algae ponds along with an electricity generation technique are provided in the present study. This is achieved through the use of Chlorella vulgaris, a fresh water algae residing in ponds, and large scale chamber-less bio- photo voltaic cell (BPV). Chlorella vulgaris was found to generate an electric current from the pond whilst causing concurrent desalination. This study proves the possibility of simultaneous power generation and salt removal using Chlorella vulgaris and opens doors for massive research potential in the fields of renewable energy and desalination.

show abstract

Effect of salt type and concentration on the growth and lipid content of Chlorella vulgaris in synthetic saline wastewater for biofuel production

Cited by 131 publications

References 34 publications

Cultivation of Chlorella sorokiniana using wastewaters from different processing units of the silk industry for enhancing biomass production and nutrient removal

Cultivation of Chlorella sorokiniana using wastewaters from different processing units of the silk industry for enhancing biomass production and nutrient removal

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Electricity Production from Desalination by Algal Ponds using Chlorella Vulgaris

Contact Info

Product

Resources

About