Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing–thawing

Shen, Tao; Jiang, Zhongliang; Liu, Hong; Li, Qingwang

doi:10.1016/j.anireprosci.2015.05.003

Cited by 14 publications

(6 citation statements)

References 37 publications

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“…Despite the encouraging results found in this study, more experiments are still needed to further improve the quality of the post‐thawed boar sperm. There might be a synergistic effect on boar sperm cryopreservation between permeable antioxidants, which were used in this study (AP and FA), and impermeable antioxidants, which were used in other studies in our laboratory, such as Laminaria japonic polysaccharide (Hu et al., ) and Salvia miltiorrhiza polysaccharides (Shen, Jiang, Liu, & Li, ), since these polysaccharides can not only scavenge ROS but also unite with sperm membrane phospholipids, replacing the surrounding H 2 O (Crowe et al., ). Then the damage to the sperm caused by the formation of ice crystals will be reduced during the period of freezing.…”

Section: Discussionmentioning

confidence: 97%

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Pei

et al. 2018

Animal Science Journal

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The main aim of the present study was to evaluate the cryoprotective effect of apigenin (AP) and ferulic acid (FA) on boar sperm during cryopreservation. AP and FA were both demonstrated to be high-efficiency antioxidants and had not previously been used to protect sperm from cryodamage. As boar sperm is sensitive to oxidative stress, suitable antioxidants are still needed for improving frozen-thawed sperm quality. With this purpose, semen samples coming from five boars were used in this study. Ejaculates of five boars were mixed and split into 16 aliquots, in which different doses of AP and FA were added separately or together. The motility, the plasma membrane integrity, the mitochondrial activity, the acrosomal integrity, the antioxidase activities and the malondialdehyde concentration of the frozen-thawed boar sperm were assessed. The results suggested that both AP and FA significantly improved the frozen-thawed boar sperm quality in all these aspects when they were added to the freezing extender separately, while the highest improvement was recorded when the extender was supplemented with 0.1 mmol/L AP plus 0.15 mmol/L FA. These findings demonstrated that supplementation of freezing extender with both AP and FA had a combined, beneficial effect on frozen-thawed boar sperm.

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Section: Discussionmentioning

confidence: 97%

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Pei

et al. 2018

Animal Science Journal

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“…As confirmed by existing studies, PCPs derived from the Chinese herbal medicine Fuling have a strong antioxidant capacity [18]. Currently, multiple Chinese herbal medicines, such as Salvia miltiorrhiza polysaccharides, Rhodiola polysaccharides, and Laminaria japonica polysaccharides, have been confirmed to have a protective effect on spermatozoa during cryopreservation [19,20]. However, there are no previous reports on the effect of different concentrations of PCPs on pig spermatozoa during cryopreservation.…”

Section: Effect Of Pcps On the Quality Of Cryopreserved Shanghai Whit...mentioning

confidence: 92%

“…In the study by Shen et al, a significant increase in the SOD activity is reported in cryopreserved boar spermatozoa after the addition of Salvia miltiorrhiza polysaccharides at 0.2-0.6 mg/mL [19]. Moreover, Hu et al find that Laminaria japonica polysaccharides also enhance the SOD enzymatic activity in the cryopreserved spermatozoa of pigs [28].…”

Section: Effect Of Pcps On the Antioxidant Function Of Cryopreserved ...mentioning

confidence: 95%

Effect of Poria cocos Mushroom Polysaccharides (PCPs) on the Quality and DNA Methylation of Cryopreserved Shanghai White Pig Spermatozoa

Zhou

Zhang

Gao

et al. 2023

Cells

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In this study, we explore the effects of Poria cocos mushroom polysaccharides (PCPs) on the quality and DNA methylation of the cryopreserved spermatozoa of Shanghai white pigs. A total of 24 ejaculates (three ejaculate samples per boar) from eight Shanghai white pigs were manually collected. The pooled semen was diluted with a based extender supplemented with different concentrations of PCPs (0, 300, 600, 900, 1200, and 1500 μg/mL). Once thawed, the quality of the spermatozoa and their antioxidant function were assessed. In the meantime, the effect of spermatozoa DNA methylation was also analyzed. The results show that compared with the control group, 600 μg/mL of PCPs significantly improves the spermatozoa viability (p < 0.05). The motility and plasma membrane integrity of the frozen–thawed spermatozoa are significantly higher after treatment with 600, 900, and 1200 μg/mL of PCPs compared with the control group (p < 0.05). In comparison with the control group, the percentages of acrosome integrity and mitochondrial activity are significantly enhanced after the application of 600 and 900 μg/mL PCPs (p < 0.05). The reactive oxygen species (ROS), the malondialdehyde (MDA) levels, and the glutathione peroxidase (GSH-Px) activity, in comparison with the control group, are significantly decreased in all groups with PCPs (all p < 0.05). The enzymatic activity of superoxide dismutase (SOD) in spermatozoa is significantly higher in the treatment with 600 μg/mL of PCPs than in the other groups (p < 0.05). As compared with the control group, a significant increase in the catalase (CAT) level is found in the groups with PCPs at 300, 600, 900, and 1200 μg/mL (all p < 0.05). In comparison with the control group, the 5-methylcytosine (5-mC) levels are significantly decreased in all groups with PCPs (all p < 0.05). As a result of these findings, a certain amount of PCPs (600–900 μg/mL) added to the cryodiluent can significantly improve the quality of Shanghai white pig spermatozoa and can also reduce the methylation of spermatozoa DNA caused by cryopreservation. This treatment strategy may establish a foundation for the cryopreservation of semen from pigs.

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“…SMP increased the expression of CAT, SOD, and GSH-Px, improved the insulin sensitivity index, and attenuated the morphological injury of the liver and pancreas to protect against the development of T2DM and improve IR. Shen et al (2015) studied the effect of SMP on boar sperm motility and found that frozen semen containing 0.4 mg/mL SMP could protect boar sperm from oxidative damage and improve sperm motility and litter size in artificial insemination of sows. As a novel broad-spectrum antibiotic for chloramphenicol in livestock, FFC could not only cause liver function damage but also lead to abnormal renal function, which may be caused by oxidative stress and abnormal renal cell apoptosis (Wang et al, 2021a;Wang et al, 2021b).…”

Section: Other Significant Effectsmentioning

confidence: 99%

Recent developments in Salvia miltiorrhiza polysaccharides: Isolation, purification, structural characteristics and biological activities

Luo

Xue²,

Zheng³

et al. 2023

Front. Pharmacol.

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In recent years, natural polysaccharides have attracted more and more attention and research because of their value in the medicine, beauty and food fields. Salvia miltiorrhiza is a traditional Chinese herb that has been used for thousands of years and has antidiabetic, antifibrotic, neuroprotective, antioxidation, anti-inflammatory and other effects. It mainly includes rosmarinic acid, tanshinone I, tanshinone IIA, tanshinone IIB, procatechualdehyde, polysaccharide and salvianolic acids. Salvia miltiorrhiza polysaccharide is a polysaccharide extracted and isolated from Salvia miltiorrhiza and has diverse biological functions, including antioxidation, anti-tumor, hepatoprotective, anti-inflammatory, immune regulatory and cardioprotective effect. In this review, the extraction, purification, structural characterization and biological activity of SMPs are summarized and new perspectives for the future work of SMPs were also proposed, we hope our research can provide a reference for further research on SMPs.

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Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing–thawing

Cited by 14 publications

References 37 publications

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Effect of Poria cocos Mushroom Polysaccharides (PCPs) on the Quality and DNA Methylation of Cryopreserved Shanghai White Pig Spermatozoa

Recent developments in Salvia miltiorrhiza polysaccharides: Isolation, purification, structural characteristics and biological activities

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