Effect of sampling time on the sampling efficiency of all-glass impinger-30 samplers for E-coli aerosol

Ding, Pei-Hsiou; Wang, Chiu‐Sen

doi:10.1016/s0021-8502(97)85335-9

Cited by 6 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because the entire E. coli suspension was nebulized in each test run, the actual number of bacteria released in the cabinet for each run was known to within an order of magnitude. Concentrations were similar to those of Hu et al (2000) and Ding and Wang (1997) in their studies involving the generation of viable bacterial aerosols. It should be appreciated that while viable cells and spores are known to be prone to damage via the nebulization process, the method discussed here is also based on the recovery of a cell fragment (i.e., the tutE or tutH genes) and its subsequent amplification via PCR.…”

Section: Release Aerosolsupporting

confidence: 68%

Novel Testing of a Biological Safety Cabinet using PCR

Fontaine¹,

Ryan

Coschigano

et al. 2010

Appl Biosaf.

View full text Add to dashboard Cite

The biological safety cabinet is commonly employed to protect both user and product in an increasing number of biotechnology applications. It is recommended that cabinets be fully certified annually, but given the requirements of good laboratory manufacturing practices, more frequent, less disruptive user checks of equipment may be desirable. Furthermore, annual field certifications only determine that cabinets meet basic design and performance parameters and cannot reveal all issues unique to a particular cabinet. This study describes the development of a novel inlaboratory test method using common biotechnology tools that is capable of assessing bioaerosols containment as well as cabinet-specific lateral motion of bioaerosols potentially responsible for product crosscontamination. Genetically engineered viable bacteria releases, followed by colony recovery and gene amplification by polymerase chain reaction, constitute the essence of the new approach. Specifically, a nebulizer was used to release a bioaerosol within a cabinet. Single-stage bioaerosol samplers were simultaneously used to sample the challenge bioaerosol containing bacteria engineered with a plasmid conferring Kanamycin resistance. Recovered challenge bacteria were grown on media containing the antibiotic, and the presence of a DNA fragment in the bacterial plasmid was confirmed. Results demonstrated that there was detectable lateral motion of bioaerosols within the biological safety cabinet tested. The importance of such lateral motion to product cross-contamination is discussed, as are the applications for this novel testing method. It is concluded that the new technique represents an enhanced level of safety cabinet field testing ability available to the end user.

show abstract

Section: Release Aerosolsupporting

confidence: 68%

Novel Testing of a Biological Safety Cabinet using PCR

Fontaine¹,

Ryan

Coschigano

et al. 2010

Appl Biosaf.

View full text Add to dashboard Cite

show abstract

“…3 and 4). A significantly decreased recovery of culturable E. coli was also reported when sampling with the AGI-30 for 5 min (Ding & Wang, 1997). Increasing the sampling duration may allow more already collected cells to be reaerosolized from the liquid (Willeke et al, 1998).…”

Section: Sampling Durationmentioning

confidence: 81%

“…The samplers with similar collection principle (e.g., AGI-30 and BioSampler with liquid fluid, Andersen 1-STG and MAS-100 with agar plate) have not been simultaneously assessed for their ability to retaining airborne L. pneumophila. Besides, the effect of sampling time on the recovery of airborne L. pneumophila remains unknown although its influence has been explored on other airborne microorganisms (Ding & Wang, 1997;Mainelis & Tabayoyong, 2010).…”

mentioning

confidence: 99%

Evaluation of bioaerosol sampling techniques for Legionella pneumophila coupled with culture assay and quantitative PCR

Chang

Chou

Hung

2010

Journal of Aerosol Science

View full text Add to dashboard Cite

“…The sampler is an all-glass, swirling aerosol collector consisting of an air inlet, three tangentially arranged nozzles and a collection vessel [35]. The AGI-30 sampler (Ace Glass Inc., N.J., USA) is a cheap, but less efficient impinger developed to sample bioaerosols [36,37].…”

Section: Collection Of Air Samplesmentioning

confidence: 99%

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

Brandl¹

2011

TOEBMJ

177

View full text Add to dashboard Cite

Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed. Bioaerosols in Indoor Environment -A Review with Special Reference to Residential and Occupational LocationsJyotshna Mandal and Helmut Brandl* Institute of Evolutionary Biology and Environmental Studies, University of Zurich, SwitzerlandAbstract: Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed.

show abstract

Effect of sampling time on the sampling efficiency of all-glass impinger-30 samplers for E-coli aerosol

Cited by 6 publications

References 2 publications

Novel Testing of a Biological Safety Cabinet using PCR

Novel Testing of a Biological Safety Cabinet using PCR

Evaluation of bioaerosol sampling techniques for Legionella pneumophila coupled with culture assay and quantitative PCR

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

Contact Info

Product

Resources

About