Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds

Ohsaki, Hideki; Tanaka, Atsuko; Hoashi, Shogo; Sasazaki, Shinji; Oyama, Kenji; Taniguchi, Masaaki; Mukai, Fumio; Mannen, Hideyuki

doi:10.1111/j.1740-0929.2009.00638.x

Cited by 65 publications

(64 citation statements)

References 31 publications

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“…This SCD gene has been extensively studied in cattle, compared with pig and chicken. The results indicated that this SCD gene has been linked to fatty acid composition in cattle (Matsuhashi et al, 2010;Ohsaki et al, 2009;Taniguchi et al, 2004). In pigs, the recent study reported the SCD gene was strongly affected in FA composition and melting point .…”

Section: Introductionmentioning

confidence: 91%

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Association of FASN and SCD genes with fatty acid composition in broilers

Maharani¹,

Seo²,

Choi³

et al. 2013

Korean Journal of Agricultural Science

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: Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

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Section: Introductionmentioning

confidence: 91%

“…The SCD gene is encoding an enzyme that catalyzes the conversion of SFA into MUFA in mammalian adipocytes (Taniguchi et al, 2004) and catalyzes the delta (△) 9 desaturation of SFA and MUFA (Ohsaki et al, 2009). This SCD also contributes the synthesis of…”

Section: Introductionmentioning

confidence: 99%

Association of FASN and SCD genes with fatty acid composition in broilers

Maharani¹,

Seo²,

Choi³

et al. 2013

Korean Journal of Agricultural Science

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“…The 84-bp in/del polymorphism in SREBP1 was deeply investigated in Asian beef cattle breeds, especially in Japanese Black (3,(8)(9)(10) and Hanwoo, also known as Korean cattle (4,7). The SS genotype frequency in Japanese Black varied from 0.07 (9) to 0.27 (10), whereas the heterozygosity level ranged between 0.47 (9) and 0.72 (10).…”

mentioning

confidence: 99%

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Proskura

2014

Turk J Vet Anim Sci

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Sterol regulatory element-binding protein 1 (SREBP1) is the main transcription factor linked to lipogenesis regulation. SREBP1 regulates the expression of the FASN and ACACA genes coding for the 2 major lipogenic enzymes involved in de novo fatty acid synthesis, fatty acid synthase and acetyl-CoA carboxylase, respectively (1). The SREBP1 pathway plays a key role in the regulation of milk fat synthesis, and thus the variation within the SREBP1 gene may influence fat content and especially fatty acid composition in bovine milk and meat (2). Hoashi et al. (3) determined the complete sequence of this gene. They discovered an insertion/deletion (ins/del) of 84 bp in intron 5 of the SREBP1 gene. The short (S)-and long (L)-type alleles were identified. Recently, several studies have indicated an association between this ins/ del polymorphism and fatty acid composition in the milk and meat of cattle (2,4-6). The aim of this study was to analyze the genotype distribution of the above-mentioned polymorphism in cattle populations of different breeds.The study included a total of 662 individuals of the following breeds: Montbeliard, Limousin, HolsteinFriesian, Angus, Jersey, and Charolais. DNA was extracted from whole peripheral blood, collected from the jugular vein into test tubes containing an anticoagulant (K 3 EDTA) using the MasterPure DNA Purification Kit for Blood (Epicentre Biotechnology). The primers used for amplification were those described previously (7). PCR amplifications were performed in a 10-µL volume of reaction mixture containing: approximately 60 ng of genomic DNA, 15 pmol of each primer, 1X Taq DNA polymerase buffer with (NH 4 ) 2 SO 4 , 1.5 mM MgCl 2 , 0.2 mM dNTP, 0.4 U of Taq DNA polymerase, and nucleasefree deionized water up to 10 µL. Amplifications were carried out according to the following temperature profile: initial denaturation at 94 °C for 5 min, followed by 35 cycles (denaturation at 94 °C for 30 s, primer annealing at 56 °C for 45 s, extension at 72 °C for 45 s) and final extension at 72 °C for 5 min. PCR products were separated in a 1.5% agarose gel in 1X TBE buffer and stained with ethidium bromide. After electrophoresis, DNA bands were visualized under UV light and photographed. Two alleles (L and S, corresponding to bands of 492 and 408 bp, respectively) and 3 genotypes (LL, LS, and SS) were identified.The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy (HolsteinFriesian, Jersey), and dual-purpose (Montbeliard) breeds. Allele and genotype frequencies obtained in the present study are given in the Table. There was no polymorphism in the SREBP1 locus in dairy cattle populations. All HolsteinFriesian and Jersey individuals were LL homozygotes. Kaneda et al. (8) also reported monomorphism inAbstract: In the present study, an 84-bp insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 (SREBP1) gene was investigated. The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy...

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“…SCD is an enzyme that catalyzes the conversion of SFA into MUFA in mammalian adipocytes (Taniguchi et al, 2004) and catalyzes the delta (∆) 9 desaturation of SFA and MUFA (Ohsaki et al, 2009). SCD also contributes the synthesis of UFAs by insertion of a cis-double bond in the delta 9 position of FA substrate (Kim and Ntambi, 1999).…”

Section: Stearoyl-coa Desaturase (Scd)mentioning

confidence: 99%

Quantitative Trait Loci and Candidate Genes Affecting Fatty Acid Composition in Cattle and Pig

Maharani¹,

Jo²,

Jeon³

et al. 2011

Korean Journal for Food Science of Animal Resources

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Investigations into fatty acid composition in meats are becoming more important due to consumer demand for high quality healthy food. Marker-assisted selection has been applied to livestock to improve meat quality by directly selecting animals for favorable alleles that affect economic traits. Quantitative trait loci affecting fatty acid composition in cattle and pigs were investigated, and five candidate genes (ACACA, FASN, SCD, FABPs, and SREBP-1) were significantly associated with fatty acid composition. The information presented here should provide valuable guidelines to detect causative mutations affecting fatty acid composition in cattle and pigs.

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Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds

Cited by 65 publications

References 31 publications

Association of FASN and SCD genes with fatty acid composition in broilers

Association of FASN and SCD genes with fatty acid composition in broilers

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Quantitative Trait Loci and Candidate Genes Affecting Fatty Acid Composition in Cattle and Pig

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