Effect of Schisandrin B on Hepatic Glutathione Antioxidant System in Mice: Protection against Carbon Tetrachloride Toxicity

Abstract

Pretreating female Balb/c mice with schisandrin B (Sch B) at increasing daily doses (1-4 mmol/kg) for 3 days caused dose-dependent increases in hepatic glutathione S-transferase (GST) and glutathione reductase (GRD) activities. However, the activities of glucose-6-phosphate dehydrogenase (G6PDH), Se-glutathione peroxidase (GPX), and gamma-glutamylcysteine synthetase (GCS) were down-regulated to varying degrees in a dose-dependent manner. While there were biphasic changes in hepatic reduced glutathione (GSH) level as well as susceptibility of hepatic tissue homogenates to in vitro peroxide-induced GSH depletion, a gradual decrease in hepatic malondialdehyde content was observed. The beneficial effect of Sch B on the hepatic GSH anti-oxidant system became more evident after CCl4 challenge. The same Sch B pretreatment regimen caused a dose-dependent protection against carbon tetrachloride (CCl4)-induced hepatotoxicity. The hepatoprotection was associated with significant enhancement in hepatic GSH status, as indicated by the substantial increase in tissue GSH levels and the corresponding decrease in susceptibility of tissue homogenates to GSH depletion. Where the activities of GST and GRD were increased linearly over non-CCl4 control values, there was also a gradual elevation in G6PDH activity upon administration of increasing doses of Sch B. In contrast, GPX activity was moderately down-regulated. The ensemble of results suggests that the hepatoprotection afforded by Sch B pretreatment may mainly be attributed to the enhancement in the functioning of the hepatic GSH anti-oxidant system, possibly through stimulating the activities of GSH related enzymes.

Gomisin N isolated from Schisandra chinensis significantly induces anti-proliferative and pro-apoptotic effects in hepatic carcinoma

“…This derivative has been used for the treatment of viral and chemical hepatitis. additionally, several studies have shown that Sch B protects against free radical-mediated hepatocellular damage and enhances hepatic glutathione antioxidant status in the mitochondria (38,39). it has been reported that schisandrin B has enantiomers [(+)Sch B and (-)Sch B] (40), which exhibit differential functions.…”

Schisandrin B Enhances Glutathione Redox Cycling and Protects Against β-amyloid-induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells: A Comparative Study of Various Phytochemicals

“…The degree of GSH depletion induced by t-BHP challenge was estimated by comparing the GSH level at 1 min post-t-BHP challenge with the basal value (i.e., at 0 min) and expressed as % depletion. GR and G6DPH activities were measured as described by Seelig and Meister [19] and Ip et al, [20] respectively.…”

Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2

“…4 Analyses of inter-specific divergence between congeneric species, populations and intra-specific variations in ITS2 sequences in Schisandra. NJ tree and BLAST1-based methods were used to identify the samples Two dendrograms were constructed on account of neighbor joining (NJ; Fig.…”

“…As a traditional medicinal herb, S. chinensis has been used as an astringent curing dry cough, asthma, night sweats, nocturnal seminal emissions and chronic diarrhea [1,2]. Modern medical research had proved that S. chinensis contains multiple active components used to protect liver [3][4][5], prevent senility [6][7][8][9], restrain oxidation [10,11], improve human body immunity ability [12], regulate the central nervous system and so on [13,14].…”