Effect of sea buckthorn protein on the intestinal microbial community in streptozotocin-induced diabetic mice

Yuan, Huaibo; Shi, Fangfang; Li, Meng; Wang, Wenjuan

doi:10.1016/j.ijbiomac.2017.09.090

Cited by 27 publications

(19 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mice were divided into five groups for an intervention study, during which all animals were fed normal chow, with or without addition. Diabetic controls (DC) received no intervention.…”

Section: Methodsmentioning

confidence: 99%

Effect of garden cress in reducing blood glucose, improving blood lipids, and reducing oxidative stress in a mouse model of diabetes induced by a high‐fat diet and streptozotocin

et al. 2020

Self Cite

View full text Add to dashboard Cite

BACKGROUND A mouse model in which diabetes mellitus was induced by low‐dose streptozotocin (STZ) injection combined with a high‐fat diet was used to study the effect of two water cress (Lepidium savitum) preparations. Diabetic mice were treated with dried cress powder or with water‐soluble extracts (tested at two doses), together with proper control groups. The mice were evaluated after 4 weeks of continuous intervention for type 2 diabetic and associated markers. We determined blood glucose, body weight, total cholesterol (TC), triglyceride (TG), low‐density lipoprotein (LDL), high‐density lipoprotein (HDL), serum insulin levels, and DNA integrity of hepatic cells. The concentrations of malondialdehyde (MDA) and lipid peroxide (LPO) and the activities of four enzymes that are part of the antioxidant defense system were determined in liver samples, as well as gene expression (by semi‐quantitative reverse transcription polymerase chain reaction) and enzyme activity of IRS‐1, IRS‐2, PI3K, AKT‐2, and GLUT4. RESULTS After 4 weeks of intervention, the levels of TC, TG, and LDL cholesterol were significantly (P < 0.5) decreased and HDL cholesterol was significantly increased. Enzyme activities of liver superoxide dismutase, glutathione, glutathione peroxidase, and catalase were significantly increased, whereas MDA and LPO concentrations were significantly reduced. The transcription level of the five genes assessed was increased, with corresponding increases in protein expression. CONCLUSION Oral uptake of garden cress can significantly reduce the blood glucose and improve the blood lipid metabolism of diabetic mice. Considerable improvements in the activity of antioxidant defense enzymes were observed in type 2 diabetic mice that improved the body's antioxidant emergency response. © 2019 Society of Chemical Industry

show abstract

“…The mice were divided into five groups for an intervention study, during which all animals were fed normal chow, with or without addition. Diabetic controls (DC) received no intervention.…”

Section: Methodsmentioning

confidence: 99%

Effect of garden cress in reducing blood glucose, improving blood lipids, and reducing oxidative stress in a mouse model of diabetes induced by a high‐fat diet and streptozotocin

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hippophae is widely used in various fields, such as the food, medicine and cosmetic industries. Hippophae rhamnoides L., the representative plant of the genus Hippophae, can be used as a food and medicine [5] (Figure 1). Studies have shown that various nutrients and bioactive components are present in Hippophae.…”

Section: Introductionmentioning

confidence: 99%

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Gong

et al. 2020

Molecules

108

View full text Add to dashboard Cite

Oxidation is a normal consequence of metabolism in biological organisms. The result is the formation of detrimental reactive oxygen species (ROS) and reactive nitrogen species (RNS). A large number of studies have shown that polyphenolic compounds have good antioxidant properties. Hippophae species plants have high polyphenolic content and are widely used in food, medicinal, or the cosmetic field. The main polyphenols in Hippophae species are flavonoids, phenolic acids and tannins, which have multiple effects. However, there is a limited number of studies on polyphenols in Hippophae species plants. This review systematically summarizes the polyphenols compounds and antioxidant activity of Hippophae species plants, and it is noteworthy that the main mechanisms of the polyphenols of Hippophae with antioxidant activity have been summarized as follows: regulating enzyme activity, affect the antioxidant reaction of cells, and others. This review provides useful information for the further study and application of Hippophae species polyphenols and their antioxidant activity.

show abstract

“…Furthermore, Bi dobacterium, Lactobacillus and Bacteroides have been shown to be bene cial bacteria that can directly affect the immune system of the host, inducing intestinal immunity and enhancing immune function [41]. Disturbance of the intestinal ora affects functional metabolites of the intestinal microbiota, such as BCAAs, short-chain fatty acids and trimethylamine-n-oxide [9,45].…”

Section: Discussionmentioning

confidence: 99%

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Gut Microbiota and Branched-Chain Amino Acid Catabolism to Attenuate Mitochondria Dysfunction

Yang

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Recent studies have reported that disruption of gut microbes and their metabolites is associated with diabetic cardiomyopathy, but the mechanism by which gut microbes improve diabetic cardiomyopathy remains unclear. Method: Male C57BL/6J mice with high-fat diet and streptozotocin-induced diabetic cardiomyopathy were studied in comparison with control littermates. Diabetic mice were either untreated or subjected to daily intragastric of pyridostigmine. After 10 weeks of hyperglycaemia, vagus activity, cardiac function and cardiac structure were measured by heart rate variability assessment, echocardiography, and immunohistochemistry. The intestinal barrier and gut microbiota were evaluated by fluorescence in situ hybridization and high-throughput sequencing. Additionally, plasma and cardiac branched-chain amino acid (BCAA) distribution and cardiac BCAA catabolism were determined. The structure and respiratory function of mitochondria were measured to assess cardiac mitochondria performance. Results: Intestinal permeability and tight junctions were impaired, bacterial translocation was increased, vagal activity was decreased in mice with diabetic cardiomyopathy mice. Additionally, gut microbes in mice with diabetic cardiomyopathy were disrupted, especially key microbes related to diabetes and BCAA production. Pyridostigmine, which reversibly inhibits cholinesterase to improve autonomic imbalance, enhanced vagus nerve activity, improved insulin resistance and cardiac damage, and alleviated intestinal barrier injury and gut microbiota disruption. Specifically, pyridostigmine decreased the abundance of diabetes-non-protective microbes and increased that of diabetes-protective microbes and BCAA-producing microbes. Pyridostigmine decreased cardiac BCAA concentrations by impairing gut microbe-mediated BCAA production. Furthermore, pyridostigmine upregulated BCAT2 and PP2Cm expression and decreased P-BCKDHA/BCKDHA and BCKDK expression, thus improving cardiac BCAA catabolism. Interestingly, the mitochondrial structural and functional disruption in mice with diabetic cardiomyopathy was attenuated after pyridostigmine administration, which may indicate one of the mechanisms by which BCAAs reduce cardiac damage. Conclusions: In conclusion, intestinal barrier, gut microbiota and vagal activity were impaired in mice with diabetic cardiomyopathy. Pyridostigmine ameliorated insulin resistance and cardiomyopathy, with an effect related to regulated gut microbes and its metabolite BCAA catabolism to attenuate mitochondria dysfunction of heart. These results provide novel insights for the development of a therapeutic strategy for diabetes-induced cardiac damage that targets gut microbes and BCAA catabolism.

show abstract

Effect of sea buckthorn protein on the intestinal microbial community in streptozotocin-induced diabetic mice

Cited by 27 publications

References 25 publications

Effect of garden cress in reducing blood glucose, improving blood lipids, and reducing oxidative stress in a mouse model of diabetes induced by a high‐fat diet and streptozotocin

Effect of garden cress in reducing blood glucose, improving blood lipids, and reducing oxidative stress in a mouse model of diabetes induced by a high‐fat diet and streptozotocin

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Gut Microbiota and Branched-Chain Amino Acid Catabolism to Attenuate Mitochondria Dysfunction

Contact Info

Product

Resources

About