Effect of seasonality and physicochemical parameters on bacterial communities in two hot spring microbial mats from Araró, Mexico

Prieto-Barajas, Cristina M.; Alfaro-Cuevas, Ruth; Valencia-Cantero, Eduardo; Santoyo, Gustavo

doi:10.1016/j.rmb.2017.07.010

Cited by 27 publications

(31 citation statements)

References 36 publications

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“…Since there was no treatment at high temperature to select for spores ( Pérez-Gutiérrez et al, 2013 ), we cannot know if the recovered strains were in a vegetative or sporulated form. The dominant bacteria in this extreme environment were firmicutes, inhabiting the microbial mats in the springs ( Prieto-Barajas et al, 2017 ). Temperature and physicochemical parameters were evaluated in different seasons and found to fluctuate between 45 and 55 °C (Bonita spring) and 63 o 74 °C (Tina hot-spring) ( Prieto-Barajas et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic plasticity and evolution of thermal tolerance in bacteria from temperate and hot spring environments

Hurtado-Bautista

Sánchez

Islas-Robles

et al. 2021

PeerJ

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Phenotypic plasticity allows individuals to respond to the selective forces of a new environment, followed by adaptive evolution. We do not know to what extent phenotypic plasticity allows thermal tolerance evolution in bacteria at the border of their physiological limits. We analyzed growth and reaction norms to temperature of strains of two bacterial lineages, Bacillus cereus sensu lato and Bacillus subtilis sensu lato, that evolved in two contrasting environments, a temperate lagoon (T) and a hot spring (H). Our results showed that despite the co-occurrence of members of both lineages in the two contrasting environments, norms of reactions to temperature exhibited a similar pattern only in strains within the lineages, suggesting fixed phenotypic plasticity. Additionally, strains from the H environment showed only two to three degrees centigrade more heat tolerance than strains from the T environment. Their viability decreased at temperatures above their optimal for growth, particularly for the B. cereus lineage. However, sporulation occurred at all temperatures, consistent with the known cell population heterogeneity that allows the Bacillus to anticipate adversity. We suggest that these mesophilic strains survive in the hot-spring as spores and complete their life cycle of germination and growth during intermittent opportunities of moderate temperatures. The limited evolutionary changes towards an increase in heat tolerance in bacteria should alert us of the negative impact of climate change on all biological cycles in the planet, which at its most basic level depends on microorganisms.

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Section: Methodsmentioning

confidence: 99%

Phenotypic plasticity and evolution of thermal tolerance in bacteria from temperate and hot spring environments

Hurtado-Bautista

Sánchez

Islas-Robles

et al. 2021

PeerJ

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“…These differences make hot springs an interesting area of research for specific biotechnological or ecological applications [4]. For this reason, several studies of hot springs placed in different countries have been reported in the literature as it is the case of Chabaane et al [5] who developed a work that attempted to enhance and optimize the potential exploitation of the Hammam Sayala thermal spring in NW Tunisia.…”

Section: Introductionmentioning

confidence: 99%

“…They found that the availability of P, K, and macronutrients like Zn, Cu, Fe, and Mg, was evaluated by means of XRD, XRF, and ICP-MS. On the other hand, a low concentration of Al in aqueous media was evidenced, which is favorable for plants due to the toxicity of this element at high concentrations. Prieto-Barajas et al [4] explored the diversity of bacterial culture communities residing in hot springs from Araró, México. They also analyzed the effect of seasonality and related changes in physicochemical parameters of spring waters.…”

Section: Introductionmentioning

confidence: 99%

Geothermal and Mineralogic Analysis of Hot Springs in the Puracé-La Mina Sector in Cauca, Colombia

Torres-Cerón

Acosta-Medina

2019

Geofluids

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Thermal waters are natural resources of great value to geothermal sciences, the tourism industry, and health. In this work, geochemical classification of physicochemical results of 17 sources at the Puracé-La Mina (Cauca, Colombia) sector was implemented in order to strengthen and determine their potential applications and enhance the continental tourism in Colombia. The analyzed parameters were developed following the Standard Methods 22nd edition, at Universidad Nacional de Colombia-Manizales. According to the results obtained by means of a geochemical classification, it was found that most of the sources have a sulfated-acid nature which makes them heated vapor waters and volcanic waters. Likewise, it was observed that all the sources are immature waters and still do not reach chemical equilibrium. On the other hand, mineralogical and chemical characterization by means of XRD and XRF showed a high content of silica isomorphous minerals with a low concentration. In addition, the presence of Fe2O3 was observed, which is insoluble at pH > 5 and remains in the rock. Nevertheless, considering that mine sources possess pH ± 2 and temperatures of 40°C, leaching is possible for iron justifying its presence in the water. Instead, elements like Na+, K+, Mg2+, and Ca2+ have high mobility at the conditions of mine sources (low pH) as a consequence of hydrolysis processes, which produce variations in water composition.

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“…En los manantiales termales de Araró se han reportado elevadas concentraciones de As, las cuales pueden alcanzar los 6.9 mg/L (Vázquez-Vázquez, Cortés-Martínez & Alfaro-Cuevas-Villanueva, 2015). En el trabajo realizado por Prieto-Barajas, Alfaro-Cuevas, Valencia-Cantero & Santoyo, (2017), se confirmaron las altas cantidades de As detectando concentraciones que van desde 2.7 a 6.6 mg/L durante las cuatro estaciones del año. El As resultó ser un factor relevante para determinar la biodiversidad bacteriana de los tapetes microbianos de las pozas, durante cuatro muestreos en diferentes épocas del año.…”

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“…Uno de los principales grupos reportados en dicho trabajo fueron las bacterias del género Bacillus, división Firmicutes. Los resultados mostraron que el As, junto con otros factores fisicoquímicos como la temperatura (≥ 50 0 C), es uno de los elementos más importantes que seleccionan las comunidades bacterianas en los manantiales termales analizados (Prieto-Barajas, Alfaro-Cuevas, Valencia-Cantero & Santoyo, 2017). En este contexto, el objetivo de este trabajo fue identificar genes (arsB,arsC,aoxB,ACR3), como un potencial mecanismo de resistencia al arsénico en cepas aisladas de los tapetes microbianos de las pozas termales de Araró, Mich. Por lo tanto, se identificó la presencia de genes ars en 21 de las 37 cepas de Bacillus analizadas y mediante la amplificación por PCR, secuenciación y análisis filogenéticos, se identificaron los genes arsB y arsC.…”

unclassified

Identificación y análisis de genes ars en cepas de Bacillus hipertolerantes al arsénico, aisladas de pozas termales en Araró, México

et al. 2018

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En este trabajo investigamos la presencia, diversidad y relaciones filogenéticas de genes asociados a la tolerancia al arsénico (As) en 37 cepas del género Bacillus, aisladas de tapetes microbianos localizados en pozas termales en Araró, Michoacán, México. Se diseñaron oligonucleótidos específicos para la amplificación por PCR de los genes arsB (bomba de expulsión de arsenito) y arsC (arsenato reductasa), ACR3 (transportador de arsenito) y aoxB (arsenito oxidasa) del género Bacillus, detectando únicamente los genes arsB y arsC en 21 de las 37 cepas analizadas (56.7% del total). Los análisis tipo Blastx demuestran una alta identidad (84-100%) con bombas de expulsión de arsenito (ArsB) y proteínas arsenato reductasas (ArsC) de diversas cepas de los géneros Bacillus, Paenibacillus, Psychrobacter y Planococcus. Dichos análisis se confirmaron a través de la construcción de filogenias de los genes arsB y arsC. La detección de los genes arsB y arsC en cepas de Bacillus se correlacionó con valores de hipertolerancia al As, los cuales correspondieron a 32 y 128 mM de arsenito (III) y arsenato (V), respectivamente. Finalmente, los genes arsB y arsC identificados en cepas de Bacillus podrían ser un mecanismo de resistencia al arsénico en un ambiente acuático extremo, como las pozas termales de Araró.

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Effect of seasonality and physicochemical parameters on bacterial communities in two hot spring microbial mats from Araró, Mexico

Cited by 27 publications

References 36 publications

Phenotypic plasticity and evolution of thermal tolerance in bacteria from temperate and hot spring environments

Phenotypic plasticity and evolution of thermal tolerance in bacteria from temperate and hot spring environments

Geothermal and Mineralogic Analysis of Hot Springs in the Puracé-La Mina Sector in Cauca, Colombia

Identificación y análisis de genes ars en cepas de Bacillus hipertolerantes al arsénico, aisladas de pozas termales en Araró, México

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