Effect of Seed Orientation and Medium Strength on &lt;i&gt;In vitro&lt;/i&gt; Germination of &lt;i&gt;Pterocarpus marsupium&lt;/i&gt; Roxb.

Mishra, Yogeshwar; Rawat, Rimi; Nema, B.K.; Shirin, Fatima

doi:10.15835/nsb549124

Cited by 9 publications

(10 citation statements)

References 15 publications

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“…These results may be ascribed to the osmotic pressure of the fullstrength MS medium. In accordance with current results, Mishra et al [2013] reported that seed germination of Pterocarpus marsupium inoculated on half strength MS medium in horizontal orientation was significantly higher than on media of other strengths. The results of incubation conditions for in vitro cultures indicated that neither the seeds cultured on the full-strength MS medium nor those cultured on halfstrength one germinated during the first two weeks when incubated at 25 ±1°C with 16 hours light and 8 hours dark.…”

Section: Discussionsupporting

confidence: 92%

IMPROVEMENT OF SILYMARIN CONTENT IN CELL CULTURES OF Silybum marianum BY COPPER SULPHATE ELICITOR

El-Sharnouby¹,

Hassan²

2018

Acta.Sci.Pol.,Hortorum Cultus

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Silybum marianum L. (Milk thistle) extracts are the main source of silymarin that is a mixture of various flavonolignan (silybin (silibinin), silydianin and silychristin). Silymarin of milk thistle has a hepatoprotective activity for liver cirrhosis and chronic inflammatory. Silybum marianum regeneration from hypocotyl explants and evaluation of their callogenesis, growth and total flavolignan (silymarin) upon copper sulphate (as abiotic elicitor) elicitation was targeted. Copper sulphate (CuSO 4) was applied in concentrations of 0, 3, 5, 7 and 9 µM to elicit the silymarin production in cultures. The elicitation periods used in this study were 2, 4, 7, 14 and 28 days. Half-strength MS medium recorded better results relative to full-strength MS one and seed incubation in the darkness at room temperature resulted in rapid germination and reached to the gar lid after 10 days. Callus fresh and dry weights as well as growth index were gradually increased with increasing the copper sulphate concentration till 5 µM while decreased thereafter at any elicitation period. With the increase of the elicitation period, the increase of the previous parameters was observed. Flavonolignan (silymarin) was positively correlated with CuSO 4 levels since all levels of copper sulphate significantly enhanced its content in relative to the control. Additionally, more silymarin was accumulated after 4 or 7 days and the accumulation significantly decreased when the elicitation period reached 14 days more. The highest silymarin (flavolignan) content (11.79 and 11.67 mg g-1 DW) was obtained when 5 or 7 µM copper sulphate levels were combined with 4 days elicitation period, being about five-fold of the control.

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Section: Discussionsupporting

confidence: 92%

IMPROVEMENT OF SILYMARIN CONTENT IN CELL CULTURES OF Silybum marianum BY COPPER SULPHATE ELICITOR

El-Sharnouby¹,

Hassan²

2018

Acta.Sci.Pol.,Hortorum Cultus

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“…The best growth in shoot length of Byrsonima cydoniifolia cultivated on different media was obtained on water-agar medium (Solanki, Siwach 2012). Seed culture of Givotia rottleriformis (Rambabu et al 2006) and Pterocarpus marsupium (Chathurani et al 2009;Mishra et al 2013) showed decrease in shoot length of seedlings germinated on full MS compared to half strength. Chathurani et al (2009) suggest that the levels of salts in MS medium may have been unnecessarily high, resulting in slowing growth or even toxic effect.…”

Section: Discussionmentioning

confidence: 99%

In vitro seed germination and node culture of the nutraceutical plant Grewia tenax

Daffalla¹,

Elsheikh²,

Ali³

et al. 2016

EEB

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Grewia tenax is an important nutraceutical plant threatened due to excessive fruit collection and physiological dormancy of seeds. In vitro propagation is required for ex situ conservation of this neglected plant. Improving germination rate is vital for providing the uniform explant material required for propagation and in vitro studies. The aim of this study was to optimize cultivation conditions for in vitro seed germination and micropropagation of G. tenax. Full seed germination was achieved in all treatments. Tests in distilled water medium showed the least number of days for germination and highest seedling length. Full Murashige and Skoog medium produced the maximum number of nodes. Darkness promoted germination rate and seedling length, but decreased the number of nodes per seedling. Benzyladenine and kinetin slightly increased the number of nodes compared to the control. Each uninodal explant cultivated on Murashige and Skoog medium free of plant growth regulators developed into a plantlet within one week. Well-rooted plants were successfully established in a greenhouse with 95% survival rate. This plant growth regulator-free system can successfully overcome seed dormancy and provide a rapid-continuous supply of plant material of G. tenax.

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“…[12]. Different basal media [10], salts strength [13], type and concentration of plant growth regulators [14], and mature and immature seed [15] have been used for germination and propagation.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Seeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae))

Koné

Nakpalo

et al. 2015

The Scientific World Journal

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Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficient in vitro regeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three types of seeds were inoculated on different basal media devoid of growth regulators. Various strengths of the medium of choice and the type and concentration of carbon source were also investigated. Responses to germination varied with the type of seed. Embryonic axis (EA) followed by seeds without coat (SWtC) germinated rapidly and expressed a high rate of germination. The growth performances of plantlets varied with the basal medium composition and the seeds type. The optimal growth performances of plants were displayed on half strength MS basal medium with SWtC and EA as source of seeds. Addition of 3% sucrose in the culture medium was more suitable for a maximum growth of plantlets derived from EA.

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Effect of Seed Orientation and Medium Strength on <i>In vitro</i> Germination of <i>Pterocarpus marsupium</i> Roxb.

Cited by 9 publications

References 15 publications

IMPROVEMENT OF SILYMARIN CONTENT IN CELL CULTURES OF Silybum marianum BY COPPER SULPHATE ELICITOR

IMPROVEMENT OF SILYMARIN CONTENT IN CELL CULTURES OF Silybum marianum BY COPPER SULPHATE ELICITOR

In vitro seed germination and node culture of the nutraceutical plant Grewia tenax

In Vitro Seeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae))

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