Effect of selected fungicides on the Fusarium growth and toxins production

Hrubošová-Hrmová, D.; Vytřasová, Jarmila; Moťková, Petra

doi:10.17221/268/2011-cjfs

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The amount of rainfall received during the period from mid-anthesis (GS65) to late milk (GS77) in the second experiment was more than double (139 mm) the amount of rainfall received in the first experiment (61 mm) during the same period, possibly resulting in washing out the fungicide and lowering of the concentrations of prothioconazole. Low concentrations of fungicides have been reported to increase the mycotoxin production by Fusarium under laboratory conditions (Magan et al 2002;Hrubošová-hrmová et al 2011) and in wheat grain (Magan et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Impact of post-anthesis rainfall, fungicide and harvesting time on the concentration of deoxynivalenol and zearalenone in wheat

Kharbikar

Dickin

Edwards

2015

Food Additives & Contaminants: Part A

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Field experiments were conducted to identify the impact of post-anthesis rainfall on the concentration of deoxynivalenol (DON) and zearalenone (ZON) in harvested wheat grain. Winter wheat plots were inoculated with Fusarium graminearum at stem extension (GS31) and prothioconazole was applied at mid-anthesis (GS65) to split plots and plots were subsequently mist irrigated for 5 days. Plots were either covered by polytunnels, irrigated by sprinklers or left as non-irrigated uncovered control plots after medium-milk (GS75). Plots were harvested either when ripe (GS92; early harvest) or three weeks later (late harvest). Fusarium head blight (FHB) was assessed each week from inoculation. At harvest, yield and grain quality was measured and grains were analysed for DON and ZON. Differences in rainfall resulted in contrasting disease pressure in the two experiments, with low FHB in the first experiment and high FHB in the second. Difference in FHB resulted in large differences in grain yield, quality and mycotoxin content. DON concentration was significantly (P < 0.05) higher in irrigated compared to covered and control plots in the first experiment, whereas in the second experiment, DON was significantly (P < 0.05) higher in the covered plots compared to the control and irrigated plots. ZON concentration was significantly (P < 0.05) higher in irrigated plots in both experiments. Later harvesting resulted in an approximate fivefold increase in ZON in the first experiment, but was not significantly different in the second experiment. Prothioconazole significantly (P < 0.05) reduced DON in both experiments, but gave inconsistent reductions to ZON. This is the first report to show that the post-anthesis rainfall can significantly increase ZON in wheat, which can increase further with a delayed harvest but may be significantly reduced with the application of prothioconazole. Importantly, in the absence of moisture late season, ZON remains at very low concentrations even when wheat is severely affected by FHB.

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Section: Discussionmentioning

confidence: 99%

Impact of post-anthesis rainfall, fungicide and harvesting time on the concentration of deoxynivalenol and zearalenone in wheat

Kharbikar

Dickin

Edwards

2015

Food Additives & Contaminants: Part A

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Impact of fungicide application to control T-2 and HT-2 toxin contamination and related Fusarium sporotrichioides and F. langsethiae producing species in durum wheat

Somma

Scarpino

Quaranta³

et al. 2022

Crop Protection

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Production of T-2 toxin and deoxynivalenol in the presence of different disinfectants

Hrubošová

Vytřasová

Brožková

2015

Potr. S. J. F. Sci.

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The aim of the work was to examine the effect of different disinfectants on production trichothecenes (especially of T-2 toxin and deoxynivalenol). Lipophilicity, chemical structure, the presence of bioactive groups and functional groups in their structure modifies biological activity and toxic potency of trichothecenes. For this reason, limits have been established designating maximum levels of mycotoxins in cereals while maintaining proper growing practices. Appropriate nutritive media were prepared with different concentration of tested disinfectants (Desanal A plus, ProCura spray and Guaa-Pool) and were inoculated using Fusarium strains. The density of Fusarium was 10 5 spores per mililitre. Nutrient media was cultivated at 15 °C and 25 °C for seven days. The strains of Fusarium graminearum CCM F-683 and Fusarium species (isolated from barley) produced quantities of deoxynivalenol. Fusarium poae CCM F-584 and Fusarium species (isolated from malthouse air) produced quantities of T-2 toxin. Desanal A plus prevented Fusarium growth and production of T-2 toxin and deoxynivalenol at the concentration 10%. It is an alkaline disinfectant on the basis of active chlorine and the surfactant that contains ˂5% of NaClO. ProCura spray at the concentration 0.6% proved to be very effective. This disinfectant contains 35% of propan-1-ol and 25% of propan-2-ol. Guaa-Pool at the concentration 0.004% proved to be very effective. It is a polymeric disinfectant with anion surface-acting agent and it contains ˂0.9% of polyhexamethylene guanidine hydrochloride and ˂0.2% of alkyl (C12-C16) dimethylbenzyl ammonium chloride. Lower contentration of disinfectants that not prevented growth of Fusarium caused higher production of T-2 toxin and deoxynivalenol. The contents of T-2 toxin and deoxynivalenol were analyzed by enzyme-linked immunosorbent assay (ELISA) using commercially produced kits (Agra Quant ® Deoxynivalenol Test kit and Agra Quant ® T-2 toxin Test kit). The experiment showed that the variability in the production of T-2 toxin and deoxynivalenol depended on the Fusarium strain used, concentration of disinfectants and temperature of cultivation.

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Effect of selected fungicides on the Fusarium growth and toxins production

Cited by 3 publications

References 17 publications

Impact of post-anthesis rainfall, fungicide and harvesting time on the concentration of deoxynivalenol and zearalenone in wheat

Impact of post-anthesis rainfall, fungicide and harvesting time on the concentration of deoxynivalenol and zearalenone in wheat

Impact of fungicide application to control T-2 and HT-2 toxin contamination and related Fusarium sporotrichioides and F. langsethiae producing species in durum wheat

Production of T-2 toxin and deoxynivalenol in the presence of different disinfectants

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