Effect of Selenium Nanoparticles and Chitosan on Production Performance and Antioxidant Integrity of Heat-Stressed Broiler

Lochi, Ghulam Murtaza; Shah, Muhammad Ghiasuddin; Gandahi, Jameel Ahmed; Gadahi, Javaid Ali; Hadi, Syed Abdul; Farooq, Tanzeela; Vistro, Waseem Ali; Rahmani, Mohammad Malyar

doi:10.1007/s12011-022-03262-y

Cited by 13 publications

(5 citation statements)

References 41 publications

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“…The relative organ weight showed significance in the selenium supplemented groups, this indicates that the stress is countered in the supplemented groups however the stressed birds did not show any reasonable growth in the visceral organ i.e., liver, gizzard, heart and proventriculus. However, Lochi presented an agreeing result stating that weight of the bursa and spleen increased significantly, in terms of relative organ weight (Lochi et al, 2023). The Se increase the number of lymphocytes by its proliferation in immune organs.…”

Section: Resultsmentioning

confidence: 92%

Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan

Ullah,

Khan

et al. 2023

SJA

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Poultry industry is confronted to different hurdles which are blamed for the decline in the nutrients supplements for humans. One of the hurdles is the oxidative stress, which is responsible for the reduction of gut morphology and growth performance in the poultry birds. The present study was performed to explore the impact of inorganic selenium (sodium selenite). Day old chicks were kept in a control environment. At day 7, five groups were made with four replicas in each group based on the diet being offered. Group A, the positive control group fed with normal basal diet group B was termed as negative control group offered basal diet + dexamethasone and group C, D and E was offered 0.2mg/kg, 0.3mg/kg, and 0.4mg/kg of basal diet, respectively. The trial lasted for 35-days and on the last day two chickens from each replicate were slaughtered (a cumulatively eight chickens from each group). Samples from different regions (duodenum, jejunum, and ileum) of the small intestine were collected and kept in the fixatives. Slices from each part were stained with H and E stain to make it fit for microscopy. Picture were taken with different magnifications, i.e., 4X, 10X and 40X and histo-morphometry was done through Pixel-Pro (Labomed America Inc). The statistical analysis was through IBM SPSS (Statistics.V22.X86). The present study concluded that selenium improves the intestinal morphology i.e. villus height, villus width, villus surface area showed better growth(P≤0.05) in the selenium supplemented groups. The FCR initially did not differ (P≥0.05) among the groups but toward the end it was comparatively better (P≤0.05) in selenium supplemented groups. The relative body weight of different organs i-e liver, gizzard, heart, pancreas, and proventriculus etc. showed significant (P≤0.05) results in each of selenium-supplemented groups. Overall, 0.3 mg/kg (Basal diet) of selenium showed better results with respect to the positive control group.

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Section: Resultsmentioning

confidence: 92%

Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan

Ullah,

Khan

et al. 2023

SJA

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“…Meanwhile, we also found that COS supplementation could alleviate heat-stress-induced poor growth performance by increasing final BW, ADG, ADFI, and feed efficiency. As a functional oligosaccharide, previous studies illustrated that COS could promote the growth performance of broilers both under thermoneutral and heat-stressed condition by improving the ADFI, intestinal function, and health status, such as through better nutrient utilization, intestinal morphology, microbiology, barrier function, oxidative status, and immunity [ 6 , 29 , 30 , 41 , 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…COS exerted an excellent anti-oxidant capacity by scavenging free radicals and terminating free radical reactions [ 50 ]. Lochi et al [ 44 ] reported that chitosan could reduce the serum MDA level, and increase serum GSH-Px and SOD activity in heat-stressed broilers. Moreover, our previous studies demonstrated that COS supplementation could alleviate heat-stress-induced oxidative damage in the spleen, liver, breast muscle, and small intestine of yellow-feather broilers [ 30 , 31 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Chitosan Oligosaccharides Alleviate Heat-Stress-Induced Lipid Metabolism Disorders by Suppressing the Oxidative Stress and Inflammatory Response in the Liver of Broilers

Lan

Luo

et al. 2023

Antioxidants

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Heat stress has been reported to induce hepatic oxidative stress and alter lipid metabolism and fat deposition in broilers. Chitosan oligosaccharides (COSs), a natural oligosaccharide, has anti-oxidant, anti-inflammatory, and lipid-lowering effects. This study is conducted to evaluate dietary COS supplementation on hepatic anti-oxidant capacity, inflammatory response, and lipid metabolism in heat-stressed broilers. The results indicate that heat-stress-induced poor (p < 0.05) growth performance and higher (p < 0.05) abdominal adiposity are alleviated by COS supplementation. Heat stress increases (p < 0.05) serum AST and ATL activity, serum and liver MDA, TG, TC, and LDL-C levels, and the expression of hepatic IL-1β, IL-6, SREBP-1c, ACC, and FAS, while it decreases (p < 0.05) serum SOD and CAT activity, liver GSH-Px and SOD activity, and the expression of hepatic Nrf2, GPX1, IL-10, MTTP, PPARα, and CPT1. Nevertheless, COS supplementation decreases (p < 0.05) serum AST and ATL activity, serum and liver MDA, TG, TC, and LDL-C levels, and the expression of hepatic IL-1β, IL-6, SREBP-1c, ACC, and FAS, while it increases (p < 0.05) serum SOD and CAT activity, liver GSH-Px activity, and the expression of hepatic Nrf2, CAT, IL-10, LPL, MTTP, PPARα, and CPT1. In conclusion, COS could alleviate heat-stress-induced lipid metabolism disorders by enhancing hepatic anti-oxidant and anti-inflammatory capacity.

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“…This may be associated with the respective antioxidant functions of Se and AOS in SeNPs-AOS. Also, the biogenic SeNPs could enhance Se bioactivity through nanoscale effects to promote the antioxidant properties [41]. Se is a key component of Se-containing proteins and enzymes, such as GSH-Px [42,43], which play a crucial role in the antioxidant defense system [44].…”

Section: Discussionmentioning

confidence: 99%

Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers

Yang,

An,

Zhang

et al. 2023

Antioxidants

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Selenium (Se) is an indispensable trace element with versatile functions in antioxidant defense in poultry. In our previous study, we synthesized a novel type of biogenic selenium nanoparticle based on alginate oligosaccharides (SeNPs-AOS), and found that the particles are sized around 80 nm with an 8% Se content, and the dietary addition of 5 mg/kg of SeNPs-AOS could effectively alleviate the deleterious effects of heat stress (HS) in broilers, but it is still unclear whether SeNPs-AOS can improve the meat quality. Therefore, the aim of this study was to evaluate the protective effects of SeNPs-AOS on breast meat quality in heat-stressed broilers, and explore the relevant mechanisms. Birds at the age of 21 days were randomly divided into four groups with six replicates per group (eight broilers per replicate) according to a 2 × 2 experimental design, using HS (33 ± 2 °C, 10 h/day vs. thermoneutral, TN, under 23 ± 1.5 °C) and SeNPs-AOS (5 mg/kg feed vs. no inclusion) as variables. The results showed that dietary SeNPs-AOS decreased the cooking loss (p < 0.05), freezing loss (p < 0.001), and shear force (p < 0.01) of breast muscle in heat-stressed broilers. The non-targeted metabolomics analysis of the breast muscle identified 78 differential metabolites between the HS and HS + SeNPs-AOS groups, mainly enriched in the arginine and proline metabolism, β-alanine metabolism, D-arginine and D-ornithine metabolism, pantothenate, and CoA biosynthesis pathways (p < 0.05). Meanwhile, supplementation with SeNPs-AOS increased the levels of the total antioxidant capacity (T-AOC), the activities of catalase (CAT) and glutathione peroxidase (GSH-Px), and decreased the content of malondialdehyde (MDA) in the breast muscle (p < 0.05) in broilers under HS exposure. Additionally, SeNPs-AOS upregulated the mRNA expression of CAT, GPX1, GPX3, heme oxygenase-1 (HO-1), masculoaponeurotic fibrosarcoma G (MafG), MafK, selenoprotein W (SELENOW), SELENOK, ferritin heavy polypeptide-1 (FTH1), Ferroportin 1 (Fpn1), and nuclear factor erythroid 2-related factor 2 (Nrf2) (p < 0.05), while it downregulated Kelch-like ECH-associated pro-36 tein 1 (Keap1) and prostaglandin-endoperoxide Synthase 2 (PTGS2) expression (p < 0.05) in broilers under HS. These findings demonstrated that the dietary addition of SeNPs-AOS mitigated HS-induced oxidative damage and metabolite changes in the breast muscle of broilers, which may be related to the regulation of the Nrf2 signaling pathway and selenoprotein synthesis. In addition, SeNPs-AOS upregulated the breast muscle gene expression of anti-ferroptosis-related molecules in broilers under HS, suggesting that SeNPs-AOS can be used as novel Se supplements against HS in broilers.

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Effect of Selenium Nanoparticles and Chitosan on Production Performance and Antioxidant Integrity of Heat-Stressed Broiler

Cited by 13 publications

References 41 publications

Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan

Effect of Inorganic Selenium Supplementation on Gut Morphology and Growth Performance in Broilers under Stress Conditions in Khyber Pakhtunkhwa, Pakistan

Chitosan Oligosaccharides Alleviate Heat-Stress-Induced Lipid Metabolism Disorders by Suppressing the Oxidative Stress and Inflammatory Response in the Liver of Broilers

Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers

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