Effect of semen collection method (artificial vagina vs. electroejaculation), extender and centrifugation on post-thaw sperm quality of Blanca-Celtibérica buck ejaculates

Jiménez-Rabadán, Pilar; Ramón, Manuel; García-Álvarez, Olga; Maroto-Morales, Alejandro; Olmo, Enrique del; Pérez-Guzmán, M. D.; Bisbal, A.; Fernández-Santos, M.R.; Garde, J.J.; Soler, Ana Josefa

doi:10.1016/j.anireprosci.2012.04.005

Cited by 72 publications

(63 citation statements)

References 39 publications

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“…It has been proven that ejaculates collected by artificial vagina have a lower percentage of sperm DNA fragmentation. Also, sperm samples collected during the breeding season in specific extenders presented better sperm quality [52]. …”

Section: Causes Of Dna Fragmentationmentioning

confidence: 99%

Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells

González-Marín

Gosálvez

Roy

2012

IJMS

283

214

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Concentration, motility and morphology are parameters commonly used to determine the fertilization potential of an ejaculate. These parameters give a general view on the quality of sperm but do not provide information about one of the most important components of the reproductive outcome: DNA. Either single or double DNA strand breaks can set the difference between fertile and infertile males. Sperm DNA fragmentation can be caused by intrinsic factors like abortive apoptosis, deficiencies in recombination, protamine imbalances or oxidative stress. Damage can also occur due to extrinsic factors such as storage temperatures, extenders, handling conditions, time after ejaculation, infections and reaction to medicines or post-testicular oxidative stress, among others. Two singular characteristics differentiate sperm from somatic cells: Protamination and absence of DNA repair. DNA repair in sperm is terminated as transcription and translation stops post-spermiogenesis, so these cells have no mechanism to repair the damage occurred during their transit through the epididymis and post-ejaculation. Oocytes and early embryos have been shown to repair sperm DNA damage, so the effect of sperm DNA fragmentation depends on the combined effects of sperm chromatin damage and the capacity of the oocyte to repair it. In this contribution we review some of these issues.

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Section: Causes Of Dna Fragmentationmentioning

confidence: 99%

Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells

González-Marín

Gosálvez

Roy

2012

IJMS

283

214

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“…Salmani et al [83] have suggested the use of soyabeanlecithin which is a suitable plant based cryoprotectant for caprine sperms. The beneficial effects of soyabean-lecithin as a substitute for egg yolk during cryopreservation of goat sperm are established in the works of Jiménez-Rabadán et al, Roof et al, Salmani et al, and Vidal et al [84][85][86][87].…”

Section: Cattlementioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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“…Thus, extenders based on soybean or extenders containing sugars with high molecular weight combined with permeable cryoprotectants such as glycerol has been studied for conventional sperm freezing in different species [2,6,[16][17][18]27,34]. Moreover, some investigations have been aimed to evaluate the effect of using permeable cryoprotectants [36], sucrose [37], or even a cryoprotectants-free technique joined to other preservation methods such as vitrification [11,13,36].…”

Section: Introductionmentioning

confidence: 99%