Effects of vitrification on ram spermatozoa using free-egg yolk extenders

Jiménez-Rabadán, Pilar; García-Álvarez, Olga; Vidal, Agustín; Maroto-Morales, Alejandro; Iniesta‐Cuerda, María; Ramón, Manuel; Olmo, Enrique del; Fernández-Santos, Rocío; Garde, J.J.

doi:10.1016/j.cryobiol.2015.05.004

Cited by 37 publications

(21 citation statements)

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“…Vitrification is a process by which liquids modify their state without the formation of ice crystals (Jiménez-Rabadán et al, 2015;Liebermann et al, 2002;Sánchez et al, 2011) acquiring a glassy or vitreous state (Gao & Critser, 2000;Katkov et al, 2006;Luyet & Hodapp, 1938;Rail & Fahy, 1985), thus presenting an alternative to conventional cryopreservation of cells or tissues. Vitrification is a process by which liquids modify their state without the formation of ice crystals (Jiménez-Rabadán et al, 2015;Liebermann et al, 2002;Sánchez et al, 2011) acquiring a glassy or vitreous state (Gao & Critser, 2000;Katkov et al, 2006;Luyet & Hodapp, 1938;Rail & Fahy, 1985), thus presenting an alternative to conventional cryopreservation of cells or tissues.…”

mentioning

confidence: 99%

“…When looking at substitute methods of cryopreservation, vitrification emerges as a possibility. Vitrification is a process by which liquids modify their state without the formation of ice crystals (Jiménez-Rabadán et al, 2015;Liebermann et al, 2002;Sánchez et al, 2011) acquiring a glassy or vitreous state (Gao & Critser, 2000;Katkov et al, 2006;Luyet & Hodapp, 1938;Rail & Fahy, 1985), thus presenting an alternative to conventional cryopreservation of cells or tissues. In addition, it is a simple procedure that requires less time and presents lower costs than conventional cryopreservation .…”

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confidence: 99%

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Porcine sperm vitrification I: cryoloops method

et al. 2016

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The aims of this study were to evaluate porcine sperm vitrification in cryoloops, with and without two different cryoprotectants and assess two warming procedures. Extended (n = 3; r = 4) and raw (n = 5; r = 2) semen was diluted in media without and with cryoprotectants (4% dimethylformamide and 4% glycerol) to a final concentration of 20 × 10 spermatozoa ml and vitrified using the cryoloops method. Two warming procedures were evaluated: rapid method (30 s at 37°C) and an ultra-rapid method (7 s at 75°C, followed by 30 s at 37°C). Total motility (phase contrast), sperm viability (6-carboxifluorescein diacetate and propidium iodide stain), membrane function (hypo-osmotic swelling test), acrosome integrity (phase contrast), chromatin condensation (toluidine blue stain) and chromatin susceptibility to acid denaturation (acridine orange stain) were evaluated before and after vitrification and analysed using Friedman's test. In all media, the only seminal parameters that were maintained after vitrification were chromatin condensation and integrity. Vitrification of porcine spermatozoon using cryoloops, both in the presence or absence of cryoprotectants and independent of the warming procedure used, permits conservation of sperm chromatin condensation and integrity. It would be interesting to further verify this by producing porcine embryos using vitrified spermatozoon with intracytoplasmic sperm injection.

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confidence: 99%

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confidence: 99%

Porcine sperm vitrification I: cryoloops method

et al. 2016

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“…The use of sugars as non‐permeable cryoprotectants has the added advantage of stabilizing cell membranes (Sánchez et al., ). Studies have been carried out using sucrose in human (Isachenko et al., ; Schulz, Muñoz, Risopatrón, & Sánchez, ), canine (Sánchez et al., ), rabbit (Rosato & Iaffaldano, ) and ram (Jiménez‐Rabadán et al., ) sperm with encouraging results in sperm motility, membrane integrity and DNA quality in vitrified‐warmed samples. Thus it would be interesting to evaluate the effectiveness of sucrose or other carbohydrates such as trehalose or raffinose for preserving porcine sperm motility, viability and acrosomes during vitrification.…”

Section: Discussionmentioning

confidence: 99%

“…Vitrification is a process by which liquids modify their state at freezing temperatures without the formation of ice crystals (Jiménez-Rabadán et al, 2015;Liebermann et al, 2002;Sánchez et al, 2011) acquiring a glassy or vitreous state (Gao & Critser, 2000;Katkov et al, 2006;Luyet & Hodapp, 1938;Rall & Fahy, 1985). Moreover, it is a simple technique that requires less time and has a greater cost-benefit than conventional cryopreservation procedures.…”

Section: Introductionmentioning

confidence: 99%

In vitro production of porcine zygotes using intracytoplasmic injection of vitrified sperm

Arraztoa

Castex

Álvarez

et al. 2017

Reprod Domestic Animals

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The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO and tri-gas. A group of porcine oocytes maturated in vitro were injected with vitrified-warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test. The injected oocytes were cultured in G1 medium at 38°C, 100% humidity and 5% CO or tri-gas. No significant differences (p > .05) were observed in embryo development between the oocytes injected with vitrified-warmed sperm (31.8%; 36/113), and those injected with semen diluted and conserved at 17°C (35.5%; 32/90), when cultured in 5% CO or under tri-gas atmosphere (42.9%; 39/91 vs. 34.2%; 26/76, respectively). No significant differences (p > .05) were observed in the percentage of pronuclei (PN) obtained between 5% CO and tri-gas, within each treatment either. Of the 52 oocytes submitted to the Sham test, only two presented a female PN (activation) indicating that the PN observed in the treatment group were a product of fertilization and not parthenogenetic activation. To conclude, porcine sperm vitrified using spheres, at a concentration of 5 × 10 spermatozoa/ml in TALP medium with 1% bovine serum albumin (BSA), conserve condensed and intact chromatin capable of producing early embryo development up to the pronuclear stage.

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“…Other significant advances in sperm technologies include cryopreservation by vitrification (22, 23), refreezing of spermatozoa (24–27), and production of spermatozoa from frozen testicular tissue (28, 29). Development in spermatogonia stem cell (SSC) culture opens a new era in the understanding of testis function and male fertility (2, 30–32).…”

Section: Male Gamete Preservation and Artificial Inseminationmentioning

confidence: 99%

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

Tibary

2017

Front. Vet. Sci.

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Effects of vitrification on ram spermatozoa using free-egg yolk extenders

Cited by 37 publications

References 39 publications

Porcine sperm vitrification I: cryoloops method

Porcine sperm vitrification I: cryoloops method

In vitro production of porcine zygotes using intracytoplasmic injection of vitrified sperm

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

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