Effect of seminal extenders containing egg yolk and glycerol on motion characteristics and fertility of stallion spermatozoa

Bedford, Sylvia J.; Jasko, D.J.; Graham, James K.; Amann, R. P.; Squires, E.L.; Pickett, B.W.

doi:10.1016/0093-691x(95)00045-a

Cited by 28 publications

(17 citation statements)

References 20 publications

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“…Similar results were obtained by Bedford et al (1995), when the addition of 4% egg yolk to milk based extender was beneficial for maintaining sperm viability of cooled semen in vitro. However, there was a decrease in fertility with the addition of egg yolk to the milk based extender demonstrated by an embryo recovery rate of only 17% for mares inseminated with extender plus 4% egg yolk compared to 50% obtained from mares inseminated with semen diluted in the milk based extender only.…”

Section: Resultssupporting

confidence: 74%

“…Grouping the results obtained by Palhares et al (1986), Silva (1988), andRossi (2008), and comparing them to those obtained by Bedford et al (1995) and those presented in this experiment, it is worth considering the existence of an interaction between removal of seminal plasma and the presence of egg yolk in seminal extenders, which additively result in damage to fertility.…”

Section: Resultsmentioning

confidence: 97%

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Physical characteristics and fertility of fractionated donkey semen cooled at 5°C

Carvalho

Filho

Palhares

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose -SMG or Lactose -Egg Yolk -LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 10 6 motile spermatozoa in a volume of 22 mL, cooled to 5°C and stored up to 48 hours in a container proposed by Palhares (1997). Sperm motility and vigor were assessed in fresh semen, after first semen dilution, before insemination, at 24 and 48 hours after storage. For the fertility evaluation, 44 mares were inseminated with semen stored for a period between 12 and 24 hours. The mares were inseminated on fixed days (Mondays, Wednesdays, and Fridays) after the detection of a follicle greater than a 30mm diameter in one of the ovaries through ovulation. Pregnancy diagnosis was performed on day 12 post-ovulation, using transrectal ultrasonography. Semen diluted in SMG showed superior sperm motility than LEY, at the Pre-AI evaluation (P<0.05). At 48 hours of storage, all donkeys had motility values between 45 and 53% for semen diluted in SMG, while only one donkey showed motility greater than 30% in the LEY treatment. The pregnancy rate/cycle for mares inseminated with semen diluted in SMG was superior than that obtained using LEY (56.52% vs 4.76%, respectively).Keywords: donkey, fractionated collection, cooled semen, fertility RESUMO Objetivou-se com o presente experimento avaliar o efeito de dois diferentes diluidores (leite em pó desnatado glicose -SMG ou lactose gema de ovo -LEY) sobre as características físicas e a fertilidade do sêmen asinino coletado de forma fracionada e resfriado a 5ºC. Para isso, quatro jumentos da raça Pêga foram utilizados como doadores de sêmen. A fração espermática rica do ejaculado foi diluída preparando-se doses inseminantes contendo 400 x 106 espermatozoides móveis em um volume de 22 mL, resfriadas a 5ºC e armazenadas por até 48 horas em contêiner proposto por Palhares (1997)

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 97%

Physical characteristics and fertility of fractionated donkey semen cooled at 5°C

Carvalho

Filho

Palhares

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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“…In the present experiment, egg yolk (which is rich in proteins), could have masked the effect of seminal plasma. Bedford et al (1995) proposed a possible negative interaction between egg yolk and seminal plasma; the mechanism is unknown, but may involve lipid peroxidation and release of free radicals, which would harm sperm and reduce their function (Bedford et al 1995). In our experiment, it is possible that the beneficial effects of seminal plasma were hidden by the presence of egg yolk.…”

Section: Discussionmentioning

confidence: 79%

Conservation of fresh ram spermatozoa at 5°C in the presence of seminal plasma

Morrier¹,

Castonguay²,

Bailey³

2003

Can. J. Anim. Sci.

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In artificial insemination, however, diluting the semen reduces the concentration of seminal plasma. To test the hypothesis that supplemental seminal plasma in extended ram semen improves conservation at 5°C, we added various concentrations of seminal plasma to semen during storage, and investigated subsequent sperm function in vitro. Semen was divided into three aliquots, extended in a commercial diluent (Triladyl) supplemented with 0, 10 or 25% (vol:vol) ovine seminal plasma and cooled to 5°C. After 8 and 24 h at 5°C, sperm were suspended in a modified synthetic oviduct fluid (SOF-m) at 39°C to mimic the female genital tract at insemination. Sperm aliquots were assessed for motility and chlortetracycline fluorescence after 0, 4 and 8 h in the SOFm. No significant differences were observed due to seminal plasma supplementation during conservation at 5°C or incubation in SOF-m at 39°C. However, decreased sperm motility and fewer non-capacitated sperm were observed concomitant with an augmentation of capacitated and acrosome-reacted cells during incubation in SOF-m. Therefore, the hypothesis that diluent supplementation with homologous seminal plasma improves ram sperm conservation or subsequent sperm function was not supported. Pour vérifier l'hypothèse qu'un supplément de plasma séminal améliorerait la conservation du sperme de bélier allongé à 5°C, les auteurs ont ajouté à ce dernier une quantité variable de plasma séminal pendant le stockage et examiné subséquemment la motilité des spermatozoïdes in vitro. Le sperme a été divisé en trois parties, allongé avec un diluant commercial (Triladyl) auquel on a ajouté 0, 10 ou 25 % (v:v) de plasma séminal d'ovin puis a été refroidi à 5°C. Après avoir passé 8 ou 24 heures à cette tempéra-ture, le sperme a été mis en suspension dans un fluide synthétique modifié imitant celui des trompes de Fallope (SOF-m) à 39°C, de manière à reproduire les conditions dans le tractus génital de la femelle à l'insémination. Les auteurs ont évalué la motilité des spermatozoïdes et la fluorescence à la chlortétracycline des aliquotes de sperme laissés 0, 4 et 8 heures dans le SOF-m. Aucune variation sensible n'a été relevée à la suite de l'addition de plasma séminal lors de la conservation du sperme à 5°C ou son incubation dans le SOF-m à 39°C. Néanmoins, la motilité réduite des spermatozoïdes et le plus petit nombre de spermatozoïdes non capacités sont cohérents avec la hausse du nombre de cellules capacitées et de cellules avec réaction acrosomique pendant l'incubation dans le SOF-m. On en déduit que la dilution du sperme de bélier avec du plasma séminal homologue n'améliore pas sa conservation ni le fonctionnement subséquent des spermatozoïdes. Mots clés:Ovins, bélier, sperme, motilité, viabilité, fluorescence à la chlortétracycline, insémination artificielle, fluide synthétique modifié des trompes de FallopeSeminal plasma facilitates sperm transport in the female genital tract [reviewed by Yanagimachi (1994)] and may regulate capacitation by contributing decapacitation factors to the...

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“…Esses resultados também estão de acordo com Gomes et al (3) e Medeiros et al (8) , que trabalharam com reprodutores Mangalarga Marchador, e Oliveira et al (12) , com garanhões da raça Crioula. A toxicidade do glicerol já foi relatada por alguns autores (13,14) , demonstrando efeito contraceptivo na égua, por reduzir a fertilidade do sêmen equino quando incluído nos diluentes usados para sêmen fresco, refrigerado e congelado. Alvarenga et al (15) observaram que o glicerol em concentrações próximas a 5% foi o crioprotetor que induziu ao maior estresse osmótico na célula espermática, levando à redução dos valores das variáveis motilidade, viabilidade e integridade de membrana, fato também observado para o sêmen criopreservado dos garanhões da raça Mangalarga Marchador com o FR5.…”

Section: Discussionunclassified

Utilização De Diferentes Diluentes Na Criopreservação De Espermatozoides De Garanhões Mangalarga Marchador

Nascimento

Blume

Oliveira³

et al. 2015

Ciênc. anim. bras.

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ResumoAs biotécnicas aplicadas à reprodução animal como inseminação artificial, transferência e produção in vitro de embriões, indução e sincronização de cio e congelamento de gametas vêm sendo cada vez mais utilizadas na prática veterinária. No entanto, algumas biotécnicas ainda não alcançaram o seu total aperfeiçoamento técnico na reprodução equina, como a criopreservação de sêmen. Objetivou-se avaliar as características pós-descongelamento (motilidade total, vigor e integridade de membrana plasmática e acrosomal) dos espermatozoides de garanhões da raça Mangalarga Marchador (n=5), empregando-se três diluentes de criopreservação. Após a colheita, o sêmen foi diluído na proporção de 1:1 em meio à base de leite em pó desnatado e centrifugado a 600 G por 10 minutos. Após a centrifugação, o sobrenadante foi desprezado e o pellet obtido dividido e ressuspendido com Botucrio, FR5 ou FR6. As amostras foram envasadas em palhetas de 0,5 mL sendo a concentração ajustada para 200x10 6 espermatozoides/mL. As palhetas foram distribuídas em uma plataforma-suporte e estabilizadas a 5 ºC/60 min., em refrigerador comercial. Para o congelamento, as palhetas, posicionadas horizontalmente, foram expostas por 15 minutos ao vapor de nitrogênio líquido, em uma caixa de isopor, a 6 cm acima do nível de nitrogênio líquido. Logo em seguida, as palhetas foram imersas no nitrogênio líquido, acondicionadas em raques e estocadas em botijão criogênico a -196 ºC, para posterior avaliação. Não houve diferença para as variáveis motilidade, vigor e integridade das membranas plasmática e acrossomais quando se utilizaram diluentes que contêm a associação de amidas e glicerol (Botucrio e FR6; P>0,05). As variáveis seminais no diluente contendo apenas glicerol foram inferiores em todas as avaliações (P<0,05). A utilização de crioprotetores como a metilformamida, em associação com concentrações de 1 ou 2%

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Effect of seminal extenders containing egg yolk and glycerol on motion characteristics and fertility of stallion spermatozoa

Cited by 28 publications

References 20 publications

Physical characteristics and fertility of fractionated donkey semen cooled at 5°C

Physical characteristics and fertility of fractionated donkey semen cooled at 5°C

Conservation of fresh ram spermatozoa at 5°C in the presence of seminal plasma

Utilização De Diferentes Diluentes Na Criopreservação De Espermatozoides De Garanhões Mangalarga Marchador

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