Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

Figueroa, Elías; Merino, O.; Risopatrón, Jennie; Isachenko, Vladimir; Sánchez, Raúl; Effer, Brian; Isachenko, Evgenia; Farías, Jorge G.; Valdebenito, Iván

doi:10.1016/j.theriogenology.2014.09.015

Cited by 74 publications

(59 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of this study indicate a positive correlation of the mitochondrial‐membrane potential with motility ( r = 0·75) and the variables V CL and V SL ( r = 0·77 and 0·79, respectively), agreeing with the results of Figueroa et al . (). This may also be related to the findings of Christen et al .…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Cryopreservation of Atlantic salmonSalmo salarsperm: effects on sperm physiology

Figueroa

Valdebenito

Merino

et al. 2016

Journal of Fish Biology

Self Cite

View full text Add to dashboard Cite

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium + 1.3M dimethyl sulphoxide + 0.3M glucose + 2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as the treatment (T) and fresh semen as the control (F). Straws of 0·5 ml of sperm suspension were frozen in 4 cm of N2 L. They were thawed in a thermoregulated bath (40° C). After thawing, the percentage of spermatozoa with fragmented DNA [transferase dUTP (deoxyuridine triphosphate) nick-end labelling (TUNEL)], plasma membrane integrity (SYBR-14/PI) and mitochondrial membrane potential (ΔΨMMit, JC-1) were evaluated by flow cytometry and motility was evaluated by optical microscope under stroboscopic light. The fertilization rates of the control and treatment semen were tested at a sperm density of 1·5 × 10(7) spermatozoa oocyte(-1) , by observation of the first cleavages after 16 h incubation at 10° C. In the cryopreserved semen (T), the mean ± s.d. DNA fragmentation was 4·8 ± 2·5%; plasma membrane integrity 75·2 ± 6·3%; mitochondrial membrane potential 51·7 ± 3·6%; motility 58·5 ± 5·3%; curved line velocity (VCL ) 61·2 ± 17·4 µm s(-1) ; average-path velocity (VAP ) 50·1 ± 17·3 µm s(-1) ; straight-line velocity (VSL ) 59·1 ± 18·4 µm s(-1) ; fertilization rate 81·6 ± 1·9%. There were significant differences in the plasma membrane integrity, mitochondrial membrane potential, motility, fertilization rate, VCL , VAP and VSL compared with the controls (P < 0·05). Also the mitochondrial membrane potential correlated with motility, fertilization rate, VCL and VSL (r = 0·75; r = 0·59; r = 0·77 and r = 0·79, respectively; P < 0·05); and the fertilization rate correlated with VCL and VSL (r = 0·59 and r = 0·55, respectively).

show abstract

Section: Discussionmentioning

confidence: 97%

“…In the current experiments, there was a positive correlation between the mitochondrial‐membrane potential and the fertilization rate, which agrees with the findings of Figueroa et al . () in cryopreserved spermatozoa of O. mykiss and S salar .…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Atlantic salmonSalmo salarsperm: effects on sperm physiology

Figueroa

Valdebenito

Merino

et al. 2016

Journal of Fish Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…; Figueroa et al . ). These techniques present various advantages: conservation of genetic material from wild animals originating from remote locations or where access is difficult; elimination of problems caused by asynchronic gonad maturity between males and females; use of gametes from animals selected for improvement programmes or subject to genetic manipulation (tetraploids, clones, transgenic material); reduction in the costs and risks of transporting live animals; and establishment of hybridisation programmes using fish with different reproduction periods (Suquet et al .…”

Section: Introductionmentioning

confidence: 97%

Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

Magnotti

Cerqueira

Lee‐Estévez

et al. 2016

Reviews in Aquaculture

View full text Add to dashboard Cite

Semen conservation methods have been extensively studied for fish species over the last 60 years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase the time over which semen samples can be used for reproduction or sperm quality analysis. Marine fish possess greater resistance than freshwater species to variations in osmolality. This favours the application of these methods, which produce better results after thawing and offer high biotechnological potential for aquaculture. In the last 15 years, sperm cryopreservation studies have been carried out in 32 marine species, but there are few analyses of the effects of freezing on the morphology and physiology of sperm cells. The object of this review was to provide recent data on semen cryopreservation in marine fish species and to suggest variables which may be applied in future research.

show abstract

“…Vitrification is a rarely used technique in fish sperm but with promising applications. Vitrification of sperm diluted in Cortland medium with 10% DMSO, 2% BSA, 0.13 M sucrose and 50% seminal plasma was reported as an option in Atlantic salmon cryopreservation (Figueroa et al, 2015). The authors reported good membrane integrity, motility and fertility and low DNA fragmentation rates under these conditions.…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

View full text Add to dashboard Cite

This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

show abstract

Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

Cited by 74 publications

References 59 publications

Cryopreservation of Atlantic salmonSalmo salarsperm: effects on sperm physiology

Cryopreservation of Atlantic salmonSalmo salarsperm: effects on sperm physiology

Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

Cryobanking of aquatic species

Contact Info

Product

Resources

About