Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

Nahak, Prasant; Nag, Kaushik; Hillier, Ashley; Devraj, Ravi; Thompson, David W.; Manna, Kausik; Makino, Kimiko; Ohshima, Hiroyuki; Nakahara, Hiromichi; Shibata, Osamu; Panda, Amiya Kumar

doi:10.5650/jos.ess14071

Cited by 9 publications

(6 citation statements)

References 43 publications

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“…The main site for such exchange is the alveolar space, which exhibits a moist and highly vascularised surface of approximately 70 m 2 . The naturally occurring fluid that bathes the alveolar lining is subject to considerable surface tension that can force structural collapse on exhalation . In order to counter this effect, and also minimise the work of breathing, a complex and highly surface active mix called pulmonary surfactant is distributed at the alveolar air‐liquid interface .…”

Section: Introductionmentioning

confidence: 99%

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The impact of cigarette/e‐cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions

Davies

Birkett

Kotwa

et al. 2017

Surface & Interface Analysis

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The impact of cigarette/e-cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions http://researchonline.ljmu.ac.uk/5333/ Article LJMU has developed LJMU Research Online for users to access the research output of the University more effectively. Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Users may download and/or print one copy of any article(s) in LJMU Research Online to facilitate their private study or for non-commercial research. You may not engage in further distribution of the material or use it for any profit-making activities or any commercial gain. LJMU Research OnlineThe version presented here may differ from the published version or from the version of the record. Please see the repository URL above for details on accessing the published version and note that access may require a subscription. Deviation in pulmonary surfactant structure-function activity can impair airway patency and lead to respiratory 8 disorders. This novel study aims to evaluate the influence cigarette / e-cigarette vapour has on model 9 surfactant films located within a simulated pulmonary environment using a lung biosimulator. 10Chromatographic analysis confirmed that nicotine levels were consistent with the sampling regimen

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Section: Introductionmentioning

confidence: 99%

“…Consequently, additional species are required in order to maintain fluidity and support surfactant respreading. For instance, palmitoyloleoylphosphatidylglycerol (POPG) facilitates effective respreading of pulmonary surfactant following compression . Commercially available lung surfactant replacement preparations (e.g.…”

Section: Introductionmentioning

confidence: 99%

The impact of cigarette/e‐cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions

Davies

Birkett

Kotwa

et al. 2017

Surface & Interface Analysis

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“…Vesicles in bulk condition can transform to monolayer at the air–water interface. − This phenomenon led to the study of the bilayer disintegration kinetics by monitoring the increase in surface pressure (π) with respect to time ( t ). , The time ( t )-dependent change in the surface pressure (π) for the different systems is shown in Figure . ILs tend to be adsorbed on the surface of the vesicles, preferentially to bind to the surface, and hence can decrease the process of disintegration, which eventually reduces the formation of the adsorbed monolayer.…”

Section: Resultsmentioning

confidence: 99%

Impact of Ionic Liquids on the Physicochemical Behavior of Vesicles

Manna,

Barai,

Mandal

et al. 2024

J. Phys. Chem. B

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The effects of two ionic liquids (ILs), 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF 4 ) and 1-butyl-1-methyl pyrrolidinium tetrafluoroborate ([bmp]BF 4 ), on a mixture of phospholipids (PLs) 1,2-dipalmitoyl-sn-glycero-3phosphatidylcholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) (6:3:1, M/M/M, 70% PL) in combination with 30 mol % cholesterol (CHOL) were investigated in the form of a solvent-spread monolayer and bilayer (vesicle). Surface pressure (π)−area (A) isotherm studies, using a Langmuir surface balance, revealed the formation of an expanded monolayer, while the cationic moiety of the IL molecules could electrostatically and hydrophobically bind to the PLs on the palisade layer. Turbidity, dynamic light scattering (size, ζ-potential, and polydispersity index), electron microscopy, small-angle X-ray/neutron scattering, fluorescence spectroscopy, and differential scanning calorimetric studies were carried out to evaluate the effects of IL on the structural organization of bilayer in the vesicles. The ILs could induce vesicle aggregation by acting as a "glue" at lower concentrations (<1.5 mM), while at higher concentrations, the ILs disrupt the bilayer structure. Besides, ILs could result in the thinning of the bilayer, evidenced from the scattering studies. Steady-state fluorescence anisotropy and lifetime studies suggest asymmetric insertion of ILs into the lipid bilayer. MTT assay using human blood lymphocytes indicates the safe application of vesicles in the presence of ILs, with a minimal toxicity of up to 2.5 mM IL in the dispersion. These results are proposed to have applications in the field of drug delivery systems with benign environmental impact.

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“…В общей выборке обследованных выявлена прямая сопряженность высокого уровня SP-A с концентрацией ХС ЛПНП > 3,0 ммоль/л, а с помощью многофакторного анализа установлено, что содержание ХС ЛПНП > 3,0 ммоль/л прямо влияет на наличие высокого уровня SP-A, увеличивая вероятность последнего в 2,2 раза. Возможно, этот эффект связан с тем, что высокий уровень сывороточного ХС ЛПНП оказывает разжижающее действие на легочной сурфактант, а также стимулирует сигнальные механизмы в альвеолярных клетках II типа [6,7], и это может способствовать увеличению поступления SP-A, составляющего 50-70 % от общего пула белков сурфактанта, в системный кровоток через альвеолярно-капиллярный барьер легких [8]. В результате этого гиперхолестеринемия, обусловленная повышенным (> 3,0 ммоль/л) уровнем ХС ЛПНП, может прямо влиять на увеличение уровня SP-A в крови у обследованных нами лиц.…”

Section: Discussionunclassified

The effect of elevated low-density lipoprotein cholesterol on surfactant proteins SP-A and SP-D content as a new mechanism of atherogenesis

Nikolaev,

Lapitskaya,

Kosarev

et al. 2023

jour

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Aim of the study was to evaluate the effect of elevated level of low-density lipoprotein cholesterol (LDL-C) on surfactant protein SP-A and SP-D content in blood, as a new factor of atherogenesis, in men and women in Novosibirsk.Materials and methods. The study included 174 people (87 men and 87 women), residents of Novosibirsk, aged 45 to 69 years. An anthropometric examination, measurement of blood pressure, determination of the lipid spectrum, blood glucose and surfactant proteins SP-A and SP-D content (enzyme immunoassay).Results. According to the results of the examination, 82 examined persons (47.1 %) were included in the group with LDL-C content > 3.0 mmol/l(main group), 92 – in the group with LDL-C content ≤ 3.0 mmol/l (comparison group) (52.9 %). The value of high SP-A and SP-D content (upper quartile) was determined, which amounted to ≥ 1413 pg/ml SP-A in men and ≥ 1649 pg/ml in women, and ≥ 1772 ng/ml SP-D in men and ≥ 1626 ng/ml in women. It was found that in the main group, the body weight of the examined persons was lower than in the comparison group, and high SP-A level was more common (p = 0.033). In the total sample a direct association of upper quartile SP-A level with LDL-C content > 3.0 mmol/l was revealed (p = 0.021). Using multivariate analysis, it was found that LDL-C concentration > 3.0 mmol/l is an independent factor that directly affects the dependent variable the presence of high SP-A level in general totality of examined individuals (odds ratio 2.20, 95 % confidence interval 1.05–4.62, p = 0.036).Conclusions. In men and women of Novosibirsk, aged 45–69 years, high blood SP-A level (≥ 1413 pg/ml in men and ≥ 1649 pg/ml in women) occurs more often at LDL-C content > 3.0 mmol/l than at LDL-C level ≤ 3.0 mmol/l. In the total sample of the examined persons, a direct association of high SP-A with LDL-C content > 3.0 mmol/l was revealed, and using multivariate analysis it was found that LDL-C concentration > 3.0 mmol/l directly affects the presence of high blood SP-A level and increases the probability of this event by 2.2 times.

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Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

Cited by 9 publications

References 43 publications

The impact of cigarette/e‐cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions

The impact of cigarette/e‐cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions

Impact of Ionic Liquids on the Physicochemical Behavior of Vesicles

The effect of elevated low-density lipoprotein cholesterol on surfactant proteins SP-A and SP-D content as a new mechanism of atherogenesis

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