Effect of sex-hormone levels, sex, body mass index and other host factors on human craniofacial bone regeneration with bioactive tricalcium phosphate grafts

Knabe, Christine; Mele, Aynur; Kann, Peter Herbert; Peleska, Barbara; Adel‐Khattab, Doaa; Renz, Harald; Reuß, Alexander; Bohner, Marc; Stiller, Michael

doi:10.1016/j.biomaterials.2017.01.035

Cited by 16 publications

(47 citation statements)

References 80 publications

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“…The bone specimens were processed using a technique that facilitated performing immunohistochemical analysis on undecalcified hard tissue sections as previously described [ 2 , 37 , 38 ]. The tissue samples were immediately fixed in an ethanol-based fixative HistoCHOICE R (AMRESCO, Solon, OH, USA.)…”

Section: Materials Und Methodsmentioning

confidence: 99%

“…Sections (50 μm) were cut using a Leitz 1600 sawing microtome (Leitz, Wetzlar, Germany) and then ground and polished. Subsequent to deacrylation of the sections by immersion in toluene, xylene and acetone, immunohistochemical staining was performed using primary mouse monoclonal antibodies specific to alkaline phosphatase (ALP) (Sigma-Aldrich, Steinheim, Germany), osteocalcin (OC) (Abcam, Cambridge, UK), and rabbit polyclonal antibodies against type I collagen (Col I) (LF-39, NIH, Bethesda, Rockville, MD, USA) and bone sialoprotein (BSP) (LF-84, NIH, Bethesda, MD, USA) [ 2 , 37 , 38 , 39 ]. Other sections were stained for Tartrate Resistant Acid Phosphatase (TRAP) activity to identify cells with osteoclastic activity using a prefabricated kit (Sigma-Aldrich, Steinheim, Germany) which is based on the method described by Goldberg and Barka (1962) [ 40 ].…”

Section: Materials Und Methodsmentioning

confidence: 99%

“…The latter ruled out the non-specific reactions of mouse and rabbit IgG to human tissues, as well as non-specific binding of the secondary antibodies and/ or peroxidase labeled polymer to human tissues. Incubation with a peroxidase labeled dextran polymer conjugated to goat anti-mouse and anti-rabbit immunoglobulins (DAKO DakoCytomation Envision+ Dual link system peroxidase, DAKO, Glostrup, Denmark) followed and the color was developed using a liquid 3-amino-9-ethylcarbazole (AEC) system (DAKO, Glostrup, Denmark), as described previously [ 2 , 37 , 38 ]. Mayer’s hematoxylin was used as a counterstain, and Kaiser’s glycerol gelatin (Merck KGaA, Darmstadt, Germany) was utilized for mounting with coverslips.…”

Section: Materials Und Methodsmentioning

confidence: 99%

“…3-amino-9-ethylcarbazole results in a bright red and/or slightly brownish color, while counterstaining with hematoxylin yields a violet/bluish color. As a result, positively stained cells and matrix components can be distinguished from cells and matrix components with negative immunostaining at the appropriate magnification [ 2 , 37 , 38 ]. Histomorphometric analysis was performed on a pair of sections 150 μm apart.…”

Section: Materials Und Methodsmentioning

confidence: 99%

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Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone-grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

Knabe

Adel‐Khattab

Kluk

et al. 2017

JFB

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This study examines the effect of a hyaluronic acid (HyAc) containing tricalcium phosphate putty scaffold material (TCP-P) and of a particulate tricalcium phosphate (TCP-G) graft on bone formation, volume stability and osteogenic marker expression in biopsies sampled 6 months after bilateral sinus floor augmentation (SFA) in 7 patients applying a split-mouth design. 10% autogenous bone chips were added to the grafting material during surgery. The grain size of the TCP granules was 700 to 1400 µm for TCP-G and 125 to 250 µm and 500 to 700 µm (ratio 1:1) for TCP-P. Biopsies were processed for immunohistochemical analysis of resin-embedded sections. Sections were stained for collagen type I (Col I), alkaline phosphatase (ALP), osteocalcin (OC) and bone sialoprotein (BSP). Furthermore, the bone area and biomaterial area fraction were determined histomorphometrically. Cone-beam CT data recorded after SFA and 6 months later were used for calculating the graft volume at these two time points. TCP-P displayed more advantageous surgical handling properties and a significantly greater bone area fraction and smaller biomaterial area fraction. This was accompanied by significantly greater expression of Col I and BSP and in osteoblasts and osteoid and a less pronounced reduction in grafting volume with TCP-P. SFA using both types of materials resulted in formation of sufficient bone volume for facilitating stable dental implant placement with all dental implants having been in function without any complications for 6 years. Since TCP-P displayed superior surgical handling properties and greater bone formation than TCP-G, without the HyAc hydrogel matrix having any adverse effect on bone formation or graft volume stability, TCP-P can be regarded as excellent grafting material for SFA in a clinical setting. The greater bone formation observed with TCP-P may be related to the difference in grain size of the TCP granules and/or the addition of the HyAc.

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Section: Materials Und Methodsmentioning

confidence: 99%

Section: Materials Und Methodsmentioning

confidence: 99%

Section: Materials Und Methodsmentioning

confidence: 99%

Section: Materials Und Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone-grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

Knabe

Adel‐Khattab

Kluk

et al. 2017

JFB

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lower estrogen levels increase bone resorption and osteoporosis (64). Increased BMI and increased estrogen levels have been shown to improve bone regeneration following maxillary bone regeneration procedures (65). Host factors likely effect the lung-bone inflammatory axis.…”

Section: Host Factorsmentioning

confidence: 99%

The Effect of Inhalant Organic Dust on Bone Health

Carrington

Poole

2018

Curr Allergy Asthma Rep

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An agriculture organic dust extract inhalation animal model has recently linked lung injury-induced inflammation to systemic bone loss. This process is dependent upon lipopolysaccharide and the toll-like receptor 4 (TLR4) signaling pathway. Downstream systemic interleukin-6 is a key mediator that subsequently activates osteoclastogenesis. Age is a host factor that impacted bone disease with younger mice demonstrating increased susceptibility to bone loss following inhalant exposures as compared to older mice. Supplemental dietary vitamin D was shown to prevent organic dust-induced bone loss, but not lung disease, in animals. Recent animal studies provide new mechanistic insight into the lung-bone inflammatory axis. Host factors, diet, and lipopolysaccharide/TLR4 signaling pathways play a significant role in explaining how inhalant organic dust exposures impact bone health. These investigations might lead to specific targeted therapeutic approaches.

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Effect of silicon‐doped calcium phosphate bone grafting materials on bone regeneration and osteogenic marker expression after implantation in the ovine scapula

Knabe

Adel‐Khattab

Hübner³

et al. 2018

J Biomed Mater Res

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Compared to the currently clinically available bone grafting materials for alveolar ridge augmentation, there is a great demand for bioactive bone substitutes with higher resorbability, which enhance osteogenesis at the same time. This has prompted the development of a silicon-doped rapidly resorbable calcium alkali orthophosphate (Si-CAOP) and silicon-doped β-tricalcium phosphate (Si-TCP). This study evaluated the effect of these two particulate graft materials as compared to the currently clinically used β-TCP on bone formation and osteogenic marker expression after 2 weeks, 1, 3, 6, 12, and 18 months of implantation in critical size defects in the sheep scapula. Immunohistochemical analysis of collagen type I, alkaline phosphatase, and osteocalcin expression was performed on resin embedded sections. The bone and particle area fraction and the bone-biomaterial contact were determined histomorphometrically. After 2 weeks and 1 month defects grafted with Si-CAOP displayed a significantly greater bone area fraction, bone-particle-contact, osteogenic marker expression and significantly lower particle area fraction than defects grafted with Si-TCP and TCP. By 3 and 6 months all materials studied mediated excellent defect regeneration with further bone remodeling at 12 and 18 months. Taken together, Si-CAOP induced the most expeditious bone regeneration of critical size defects in the sheep scapula. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

show abstract

Effect of sex-hormone levels, sex, body mass index and other host factors on human craniofacial bone regeneration with bioactive tricalcium phosphate grafts

Cited by 16 publications

References 80 publications

Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone-grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

Effect of a Particulate and a Putty-Like Tricalcium Phosphate-Based Bone-grafting Material on Bone Formation, Volume Stability and Osteogenic Marker Expression after Bilateral Sinus Floor Augmentation in Humans

The Effect of Inhalant Organic Dust on Bone Health

Effect of silicon‐doped calcium phosphate bone grafting materials on bone regeneration and osteogenic marker expression after implantation in the ovine scapula

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