Effect of Shin’iseihaito (Xinyiqingfeitang) on Acute <i>Streptococcus pneumoniae</i> Murine Sinusitis via Macrophage Activation

Minami, Masaaki; Konishi, Toru; Takase, Hiroshi; Jiang, Zhixia; Arai, Tamio; Makino, Toshiaki

doi:10.1155/2017/4293291

Cited by 15 publications

(15 citation statements)

References 28 publications

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“…Mice were purchased from Japan SLC (Hamamatsu, Japan). The ability of the colonized effect of MRSA in mice after nasal inoculation was assessed using a previous procedure [ 15 ]. In brief, bacteria were harvested after 16 h of growth on TSAII sheep blood agar, and were mixed in 1 mL of phosphate buffered saline (PBS, pH 7.2, 0.15 M), then centrifuged at 2000× g for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hochuekkito (Buzhongyiqitang) on Nasal Cavity Colonization of Methicillin-Resistant Staphylococcus aureus in Murine Model

2018

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) infections are largely preceded by colonization with MRSA. Hochuekkito is the formula composing 10 herbal medicines in traditional Kampo medicine to treat infirmity and to stimulate immune functions. We evaluated the efficacy of hochuekkito extract (HET) against MRSA colonization using a nasal infection murine model. Methods: We evaluated the effects of HET as follows: (1) the growth inhibition by measuring turbidity of bacterial culture in vitro, (2) the nasal colonization of MRSA by measuring bacterial counts, and (3) the splenocyte proliferation in mice orally treated with HET by the 3H-thymidine uptake assay. Results: HET significant inhibited the growth of MRSA. The colony forming unit (CFU) in the nasal fluid of HET-treated mice was significantly lower than that of HET-untreated mice. When each single crude drug—Astragali radix, Bupleuri radix, Zingiberis rhizoma, and Cimicifugae rhizome—was removed from hochuekkito formula, the effect of the formula significantly weakened. The uptake of 3H-thymidine into murine splenocytes treated with HET was significantly higher than that from untreated mice. The effects of the modified formula described above were also significantly weaker than those of the original formula. Conclusions: Hochuekkito is effective for the treatment of MRSA nasal colonization in the murine model. We suggest HET as the therapeutic candidate for effective therapy on nasal cavity colonization of MRSA in humans.

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Section: Methodsmentioning

confidence: 99%

Effect of Hochuekkito (Buzhongyiqitang) on Nasal Cavity Colonization of Methicillin-Resistant Staphylococcus aureus in Murine Model

2018

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“…The effect of SSHT on the S. pyogenes -induced murine sinusitis model after nasal inoculation was assessed using the following procedure [ 15 ]. S. pyogenes were cultured for 16 h on TSAII sheep blood agar, harvested, and mixed in 1 mL of PBS followed by centrifugation at 2,000 ×g for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…Procedures to obtain nasal cultures have been described previously [ 15 ]. In brief, the mice were sacrificed by CO 2 inhalation.…”

Section: Methodsmentioning

confidence: 99%

“…Clinical investigation of the characteristics of adjunctive TCM use in patients with chronic rhinosinusitis showed that the most common Chinese herbal formula used was Xinyiqingfeitang in Taiwan [ 11 ]. In our previous studies, we investigated the antibacterial activity of the Shin'iseihaito extract (SSHT) against S. pneumoniae in vitro and the preventive effect of SSHT in an ovalbumin-induced allergic rhinitis model [ 12 – 15 ]. In principle, most Kampo formulas are orally administered in Japan.…”

Section: Introductionmentioning

confidence: 99%

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Comparison between the Effects of Oral and Intramuscular Administration of Shin’iseihaito (Xinyiqingfeitang) in a Streptococcus pyogenes‐Induced Murine Sinusitis Model

Minami

Konishi

Takase

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Streptococcus pyogenes (S. pyogenes) is a species of Gram-positive coccoid bacteria having many virulence factors. Its capsule and exotoxins can cause upper respiratory tract infections such as sinusitis. The general treatment for S. pyogenes-induced sinusitis is administration of antibiotics such as penicillin and macrolides; however, a serious problem associated with these antibiotics is their attenuated effect. Shin'iseihaito (Xinyiqingfeitang), a formula of Japanese traditional Kampo medicine and traditional Chinese medicine, has been used for the treatment of sinusitis. In general, formulas of Japanese traditional Kampo medicine are orally administered. This is in contrast to certain formulas of traditional Chinese medicine, which are being recently administered intramuscularly or intravenously. Regarding these traditional Chinese medicine formulas, the injection methodology is reported to be more effective than oral intake. In this study, we compared the efficacy between orally and intramuscularly administered Shin'iseihaito against S. pyogenes-induced sinusitis. We evaluated the antibacterial effect of Shin'iseihaito extract (SSHT) against S. pyogenes by K-B disk diffusion assay. Furthermore, we investigated the nasal colonization of S. pyogenes, determined cytokine (TNF-α, IL-1β, and IL-6) levels, and conducted a splenocyte proliferative assay in a murine sinusitis model. SSHT displayed direct anti-S. pyogenes activity. Intramuscular administration of SSHT decreased the nasal colonization of S. pyogenes compared with oral administration. Thymidine uptake analysis revealed that the proliferation of splenocytes from S. pyogenes-infected mice under intramuscular SSHT treatment was upregulated compared to that of splenocytes from S. pyogenes-infected mice under oral SSHT treatment. We also found that TNF-α, IL-1β, and IL-6 levels in the nasal discharge from intramuscularly treated S. pyogenes-infected mice were lower than those from orally treated mice. Our findings suggest that intramuscular administration of Shin'iseihaito may be useful for the treatment of murine S. pyogenes-induced sinusitis.

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“…Ex Vivo animal studies were performed with some modifications [12]. Serum and intraperitoneal macrophages were collected from mice treated with oral LJFE (2 g/kg body weight), CGA (9.2 mg/kg body weight), or saline once a day for seven days.…”

Section: Analysis Of Ex Vivo Effects Of Ljfe On Immune Functionsmentioning

confidence: 99%

Effects of Lonicera japonica Flower Bud Extract on Citrobacter rodentium-Induced Digestive Tract Infection

Minami

Makino

2020

Medicines

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Background: Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic Escherichia coli, in which the Shiga toxin is overproduced by antibiotic action. The Lonicera japonica flower bud (LJF) is an herbal component used against purulent diseases in traditional Japanese and Chinese medicine. We investigated the effects of LJF extract (LJFE) on Citrobacter rodentium-induced digestive tract infection in a mouse model. Methods:Citrobacter rodentium and LJFE were orally administered to C57BL/6 mice. The survival rate and bacterial colonization in the large intestine, mesenteric lymph node, and blood of mice were evaluated. Cytokines secreted from intraperitoneal macrophages of LJFE-treated mice were measured using ELISA. Moreover, the phagocytic activity of intraperitoneal macrophages against Citrobacter rodentium was compared between LJFE- or chlorogenic acid (CGA)-treated mice. Results: LJFE significantly increased the survival rate and decreased Citrobacter rodentium colonization in mice. Moreover, the values of tumor necrosis factor-α, interleukin-1β, and interferon-γ secreted from macrophages were increased following LJFE treatment. While macrophages of LJFE-treated mice showed a significant phagocytic activity, macrophages of CGA-treated mice only showed a phagocytic tendency. Conclusions: LJF may be useful for treating Citrobacter rodentium-induced digestive tract infection.

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Effect of Shin’iseihaito (Xinyiqingfeitang) on Acute Streptococcus pneumoniae Murine Sinusitis via Macrophage Activation

Cited by 15 publications

References 28 publications

Effect of Hochuekkito (Buzhongyiqitang) on Nasal Cavity Colonization of Methicillin-Resistant Staphylococcus aureus in Murine Model

Effect of Hochuekkito (Buzhongyiqitang) on Nasal Cavity Colonization of Methicillin-Resistant Staphylococcus aureus in Murine Model

Comparison between the Effects of Oral and Intramuscular Administration of Shin’iseihaito (Xinyiqingfeitang) in a Streptococcus pyogenes‐Induced Murine Sinusitis Model

Effects of Lonicera japonica Flower Bud Extract on Citrobacter rodentium-Induced Digestive Tract Infection

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