Effect of short-term cryostimulation on antioxidative status and its clinical applications in humans

Abstract

Whole body cryostimulation (WBCT) is becoming popular in medicine and sport as an adjuvant form of treatment since late 1970s. Only a few works concerning antioxidant protection after WBCT have been published. The aim of this study was to determine the effect of a ten 3-min-long exposures (one exposure per day) to cryogenic temperature (−130°C) on the level of total antioxidant status (TAS), activity of selected antioxidant enzyme superoxide dismutase (SOD) and main non-enzymatic antioxidant—uric acid (UA) in WBCT study group (man n = 24; female n = 22) and non-WBCT control subjects (man n = 22; female n = 26). Moreover, we evaluated the lipid peroxidation measured as thiobarbituric acid reactive substances products. Their blood samples were collected twice at an interval of 10 days in both study group and control subjects. The activity of antioxidant enzyme and lipid peroxidation was assayed in erythrocytes, while the concentration of uric acid was measured in plasma. After completing a total of ten WBCT sessions a significant increase (p < 0.001) of TAS and UA levels in plasma (p < 0.001) in comparison to non-WBCT was observed. Our data showed that there was statistically significant increase of the activities of SOD in erythrocytes obtained from WBCT study group compared to non-WBCT controls after 10 days of treatment (p < 0.001). It was concluded that expositions to extremely low temperatures use in cryostimulation improve the antioxidant capacity of organism.

Whole-Body Cryostimulation as an Effective Method of Reducing Oxidative Stress in Healthy Men

“…Miller et al [32] observed that 10 sessions of WBC (temperature: -130°C, time: 3 min, without subsequent kinesiotherapy) may reduce the generation of different reactive oxygen and nitrogen species in healthy subjects, due to an increase in the plasma level of SOD, UA and TAS. The authors also found a significant increase in the thiobarbituric acid reactive substance (TBARS) concentration as a marker of lipid peroxidation in healthy men who underwent WBC, while in women the concentration of TBARS did not change significantly after the completion of WBC procedures.…”

Cryotherapy in inflammatory rheumatic diseases: a systematic review

“…Vasoconstriction (α-adrenoreceptors) [76] Cold receptors Afferent nerve CNS ↓ Collagenases [41] ↓ MMP [39,40] ↓ PGE2 [77,78] ↓ IL-1β [24,35] ↓ IL-6 [69] ↓ TNF-α [24] ↓ i-NOS ↓ MPO [24,32] ↓ SOD [79] ↓ GSH [80] Decreased oxydative stress ↓ IL-10 [77] ↓ VEGF [36] Decreased angiogenesis Decreased enzymatic activities…”

An observational study on the relationship between plasma vitamin C, blood glucose, oxidative stress, endothelial dysfunction and outcome in patients with septic shock

“…Compared with the normal reference range of 1.9 -3.9 nmol/mL, [15] the median TBARS level in the patients as a marker of oxidative stress was high at baseline (19.5 (14.0 -37.0) nmol/mL) and continued to be high at day 1 (20.4 (13.0 -64.0) nmol/mL), with no statistically significant reduction at day 7 (13.3 (9.0 -18.0) nmol/mL, p=0.52). At baseline, non-survivors of septic shock had higher median TBARS levels (mmol/ mL) than survivors (16.9 (11.9 -21.7) v. 43.8 (23.6 -47.7) mmol/mL, p=0.008).…”