Effect of short-term dietary administration of eugenol in humans

Abstract

<jats:p> 1 In order to study the antigenotoxic potential of eugenol in humans, ten healthy non-smoking males ingested a daily amount of 150 mg eugenol or the placebo for seven consecutive days. After a washout period of one week, groups ingesting eugenol or the placebo were crossed and received the other treatment for seven consecutive days. </jats:p><jats:p> 2 On days 8 and 22 blood samples were taken for the assessment of standard clinical biochemical parameters. To study the possible antigenotoxic effect of eugenol, on day 8 and 22 blood samples were collected and exposed in vitro to the established genotoxic agents mitomycin C and vinblastine. After exposure the percentage of cells with chromosome aberrations and micronuclei was deter mined in cultured white blood cells. On days 8 and 22 paracetamol (500 mg p.o.) was administered as test substance to measure phase-II biotransformation capa city. Glutathione-S-transferase (GST) activities were determined in erythrocytes and blood plasma. </jats:p><jats:p> 3 No significant differences in the clinical biochemical parameters were detected between the eugenol-period and the placebo-period, indicating that daily administration of 150 mg eugenol for 7 days has no toxic affects. </jats:p><jats:p> 4 No significant differences on the cytogenetic para meters were found after ingestion of eugenol. Thus, there are no indications for an antigenotoxic potential of eugenol in humans, consuming daily 150 mg eugenol for 7 days. </jats:p><jats:p> 5 A significant reduction in α-class GSTs in plasma (P&lt;0.05), but not in the other measured biotransformation parameters, was found in volunteers during the eugenol- period as compared to the placebo-period. This may either reflect GST-inhibition by eugenol or protection against background damage of liver cells by eugenol. </jats:p>

Ethnopharmacological Studies on Allspice (Pimenta dioica) in Laboratory Animals

“…Eugenol was found to inhibit lipid peroxidation (Rajkumar & Rao, 1993). Eugenol has also been shown to possess antioxidant activity in human beings (Rompelberg et al, 1996).…”

Effect of a vegan diet on biomarkers of chemoprevention in females

“…The technique for measuring micronuclei in binucleated cells was as described by Rompelberg et al 18 In short duplicate cultures were treated with mitomycin C (0.3 pg/ml for 2 h at 37°C); control cultures were exposed to the solvent (saline) only.…”

Estudo toxicológico pré-clínico (agudo) do extrato do Syzygium aromaticum (L) em roedores

“…No caso do uso do extrato nas composições de materiais restauradores certamente o risco de ingestão aguda é reduzido do mesmo modo como a toxicidade se aplica para outros materiais restauradores. Além disso, há indícios que o risco de toxicidade por ingestão de 150 mg de eugenol por um período de sete dias é baixo (Rompelberg et al, 1996). Desta forma, existe um baixo risco de toxicidade por ingestão de eugenol na saliva quando originário de cimentos restauradores provisórios a base de eugenol porque estes cimentos restauradores não liberam elevadas quantidades deste composto para a saliva de forma prolongada.…”