Effect of shosaikoto, a japanese and chinese traditional herbal medicinal mixture, on the mitogenic activity of lipopolysaccharide: A new pharmacological testing method

Iwama, Hiroko; Amagaya, Sakae; Ogihara, Yukio

doi:10.1016/0378-8741(87)90093-6

Cited by 64 publications

(19 citation statements)

References 10 publications

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“…Traditional Chinese medicine is orally administered to animals, blood is collected to separate the serum, and then drug serum is applied for experimental analysis in vitro. Serum pharmacology has been wildly used for pharmacology studies of traditional Chinese medicine in vitro [25–27]. …”

Section: Discussionmentioning

confidence: 99%

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

Liu

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Simiao pill, a Chinese herbal formula containing four herbs, has been used in the treatment of gouty arthritis for many years. The aim of this study was to explore the effects of modified Simiao decoction (MSD) on IL-1β and TNFα secretion in monocytic THP-1 cells with monosodium urate (MSU) crystals-induced inflammation. The MSU crystals-induced inflammation model in THP-1 cells was successfully established by the stimulation of phorbol 12-myristate 13-acetate (PMA) and MSU crystals. Then, the MSD-derived serum or control serum extracted from rat was administered to different treatment groups. The morphology of MSU crystals and THP-1 cells was observed. IL-1β and TNFα protein expression in supernatant of THP-1 cells were determined by ELISA. Our data demonstrated that MSU crystals induced time-dependent increase of IL-1β and TNFα. Moreover, MSD significantly decreased IL-1β release in THP-1 cells with MSU crystals-induced inflammation. These results suggest that MSD is promising in the treatment of MSU crystals-induced inflammation in THP-1 cells. MSD may act as an anti-IL-1 agent in treating gout. The underlying mechanism may be related to NALP3 inflammasome which needs to be validated in future studies.

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Section: Discussionmentioning

confidence: 99%

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

Liu

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Furthermore, it has been reported that Gecko has an anti-tumor effect on several cancers including gastric cancer, liver cancer and esophageal carcinoma [3-5]. However, due to the uncertain scientific backgrounds of these therapeutic effects, the Gecko still may not gain global reliability as an anti-cancer drug, although several attempts have been made to develop new anti-cancer pharmaceuticals from Chinese herbal medicine [6-8]. …”

Section: Introductionmentioning

confidence: 99%

GeckoProteins Exert Anti-Tumor Effect against Cervical Cancer Cells Via PI3-Kinase/Akt Pathway

Jeong

Chung

Kim

et al. 2012

Korean J Physiol Pharmacol

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Anti-tumor activity of the proteins from Gecko (GP) on cervical cancer cells, and its signaling mechanisms were assessed by viable cell counting, propidium iodide (PI) staining, and Western blot analysis. GP induced the cell death of HeLa cells in a dose-dependent manner while it did not affect the viability of normal cells. Western blot analysis showed that GP decreased the activation of Akt, and co-administration of GP and Akt inhibitors synergistically exerted anti-tumor activities on HeLa cells, suggesting the involvement of PI3-kinase/Akt pathway in GP-induced cell death of the cancer cells. Indeed, the cytotoxic effect of GP against HeLa cells was inhibited by overexpression of constituvely active form of Akt in HeLa cells. The candidates of the functional proteins in GP were analyzed by Mass-spectrum. Taken together, our results suggest that GP elicits anti-tumor activity against HeLa cells by inhibition of PI3-kinase/Akt pathway.

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“…We used pooled sera to avoid variance or detection errors, as the mean values of pooled serum samples were much closer to one another than the mean values of single serum samples. 18 According to the results of Iwama, 17 Yang Guang and others, the effects of mixed sera are similar to those of a single serum. In addition, our use of pooled sera was based on ethical considerations, in that completing our experiments required less blood from each of the healthy subjects.…”

Section: Introductionmentioning

confidence: 84%

Effect of serum from healthy individuals on the growth of melanocytes in vitro following moxibustion at the “Jiudianfeng” point

Liu

Niu

et al. 2020

J Int Med Res

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Objective: To investigate the effects of serum from healthy individuals obtained following moxibustion at the "Jiudianfeng" point on melanocytes in vitro. Methods: Ten healthy adults (five male and five female) were treated by moxibustion at the "Jiudianfeng" point for 30 minutes once daily for 3 months. The effects of treatment with serum obtained following moxibustion on melanocyte proliferation, melanin content, tyrosinase activity, cell cycle progression, and c-kit mRNA and protein expression were assessed in vitro before and after moxibustion for 1, 2, and 3 months. Results: Exposure to sera from healthy adults following moxibustion therapy promoted melanocyte proliferation, melanin synthesis, tyrosinase activity, and c-kit mRNA and protein expression in vitro. Melanin synthesis and tyrosinase activity increased in the first 2 months following moxibustion and a synchronous decline was observed during the third month. Serum also promoted melanocyte entry into the G1 phase of the cell cycle. Conclusions: Serum treatment following moxibustion at the "Jiudianfeng" point promoted melanocyte proliferation and melanin synthesis. Further exploration of this intriguing phenomenon is essential.

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Effect of shosaikoto, a japanese and chinese traditional herbal medicinal mixture, on the mitogenic activity of lipopolysaccharide: A new pharmacological testing method

Cited by 64 publications

References 10 publications

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

GeckoProteins Exert Anti-Tumor Effect against Cervical Cancer Cells Via PI3-Kinase/Akt Pathway

Effect of serum from healthy individuals on the growth of melanocytes in vitro following moxibustion at the “Jiudianfeng” point

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