Effect of Silica Nanoparticles on level Cyp19a1and Cyp17a1genes in Male Rats

Abstract

The current study has been designed to evaluate the effect of silica Oxide nanoparticles (SiO2NPs) exposure on the reproductive performance of male rats. Forty adult Wistar albino male rats ( aged 60-70 days with body weight 130-140 g), were randomly divided into four equal groups, 10 rats per group: control group were received physiological saline, and three treated groups were administered by gavage at dose (1 mg, 10 mg and 100 mg/kg Body weight) of SiO2NPs suspension daily for 22 days, to study the effect of treated groups with silica nanoparticles on the expression of mRNA for Cyp19a1and Cyp17a1genes in testis by using qRT-PCR technique. Experimental results obtained from SiO2NPs treated male rats at the molecular level, the results appear that there is a significant (p<0.05) decrease in expression level of Cyp19a1and Cyp17a1genes in testis tissue for groups treated compared to control group.