Effect of Simmental bull seminal plasma protein in egg yolk-citrate extender on Kacang buck semen fertility

Susilowati, Suherni; Triana, Indah Norma; Sardjito, Trilas; Suprayogi, Tri Wahyu; Wurlina, Wurlina; Mustofa, Imam

doi:10.1016/j.cryobiol.2020.10.013

Cited by 22 publications

(38 citation statements)

References 53 publications

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“…Sperm DNA integrity is essential for embryo development [ 62 ]. Lower SDF of sperm, followed by a higher pregnancy rate [ 3 ]. Sperm with SDF can complete the fertilization process.…”

Section: Discussionmentioning

confidence: 99%

“…The extended semen was packaged in 0.25 mL French straws at 60 million sperm/straw. The filled straws were placed on the Cold Handling Cabinet (Minitube), held in liquid nitrogen vapor (−140 °C) for 10 min, immersed, and stored immediately in liquid nitrogen (−196 °C) for two weeks before it was assessed [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…The intact plasma membranes were marked by circular tails; meanwhile, the damaged plasma membranes were characterized by straight tails. The thiobarbituric acid method was used for MDA concentration measurement [ 3 ]. Toluidine blue staining was used for SDF assessment.…”

Section: Methodsmentioning

confidence: 99%

“…Kacang buck's sperm is more sensitive to cryo-damaged than Ettawa goat, ram, and Simmental bull's semen. Post-thawed of Kacang buck semen, which extended without any antioxidant, about 60% of the sperm die, leaving less than 40% of the motile sperm [ 3 ]. This post-thawed motility has not met the artificial insemination (AI) requirement, which must more than 40% [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…DNA integrity is necessary for embryo development [ 10 ]. Higher sperm SDF resulted in lower fertilization rates [ 11 ], lower pregnancy rate [ 12 ], and finally, lower pregnancy rate [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

Mustofa

Susilowati

Wurlina

et al. 2021

Heliyon

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The study aimed to determine the addition of green tea extract (GTE) in extender on the quality and DNA mutation of post-thawed Kacang buck sperm. The sperm DNA mutation was observed on nicotinamide adenine dinucleotide hydride (NADH) dehydrogenase 1 (ND1) of mitochondrial Deoxyribonucleic Acid (mtDNA). A pool of 12 Kacang buck ejaculates was diluted in skim milk-egg yolk extender contained 0, 0.05, 0.10, and 0.15 mg of GTE/100 mL for T0, T1, T2, and T3 group, respectively. Each of the aliquot groups was packaged in 0.25 mL French mini straw contained 60 million alive sperm and froze according to the protocol. The ND1 mtDNA amplification of samples was carried out Polymerase Chain Reaction machine, followed by DNA sequencing using the Sanger method. Meanwhile, the phylogenetic tree was constructed using the neighbor-joining (NJ) method with MEGA 7.0 software. The results showed that the T2 group maintained the highest quality for Kacang buck post-thawed semen. There was the highest percentages of sperms viability, motility, intact plasma membrane (IPM), the lowest of malondialdehyde (MDA) concentration, sperm DNA fragmentation (SDF), the total and types of ND1 mtDNA mutation frequency. The phylogenetic tree analysis revealed that the clade of the T2 group was most closely related to the sequence reference. However, there was no correlation between the semen quality parameters (sperm viability, motility, IPM, MDA concentration, and SDF) with ND1 mtDNA mutation of post-thawed Kacang buck semen. It could be concluded that GTE was useful as an antioxidant for Kacang buck semen extender for frozen sperm.

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“…Sperm DNA integrity is essential for embryo development [ 62 ]. Lower SDF of sperm, followed by a higher pregnancy rate [ 3 ]. Sperm with SDF can complete the fertilization process.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%