Effect of single and multiple doses of low-level laser therapy on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED)

Almeida-Júnior, Luciano Aparecido de; Marques, Nádia Carolina Teixeira; Prado, Mariel Tavares de Oliveira; Oliveira, Thaís Marchini de; Sakai, Vivien Thiemy

doi:10.1007/s10103-019-02836-y

Cited by 19 publications

(7 citation statements)

References 46 publications

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“…The results also were detailed, with all experimental groups having a high percentage of cellular viability post‐irradiation, confirmed by the trypan blue exclusion assay. A related result was reported in the studies that had an increase in cellular viability, proliferation, and differentiation after using photobiomodulation (980 and 660 nm) [43,44].…”

Section: Discussionsupporting

confidence: 60%

Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

2020

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Background and Objectives Temporomandibular disorder (TMD) is an incapacitating disease with temporomandibular joint (TMJ) disc degenerative changes in patients. Despite several research attempts to find a definitive treatment, there is no evidence of a permanent solution. The objective of the current study was to observe the role of 660 nm diode laser in the differentiation of human adipose‐derived stem cells (ADSCs) to fibroblasts and chondrocytes. Study Design/Materials and Methods After irradiation, the morphology, viability, and adenosine triphosphate (ATP) proliferation of the ADSCs were analyzed at different time intervals. The differentiation of ADSCs toward fibroblastic and chondrogenic phenotypes was supported using flow cytometry and immunofluorescence at 1‐ and 2‐week post‐irradiation. Results More than 90% of viable cells were observed in all experimental groups, with an increase in ATP proliferation. Flow cytometry analyses and immunofluorescence demonstrated the presence of chondrogenic and fibroblastic cell surface markers at 1‐ and 2‐week post‐irradiation. Conclusion This study has demonstrated methods to induce the differentiation of ADSCs toward fibroblastic and chondrogenic phenotypes with a 660 nm diode laser. The study also proposes a future alternative method of treatment for patients with degenerative TMJ disc disorders and presents a positive prospect in the application of photobiomodulation and ADSCs in the treatment of degenerative TMJ disc. Lasers Surg. Med. © 2020 Wiley Periodicals LLC

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Section: Discussionsupporting

confidence: 60%

Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

2020

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“…LLLT (Ga-As laser, 804 nm, 1 and 3 J/cm 2 ) treated mesenchymal stem and cardiac stem cells had increased cell proliferation up to 4 and 2 weeks post-LLLT, respectively, and there were no differences between the two energy densities (19). Stem cells from human exfoliated deciduous teeth treated with LLLT (660 nm, 2.5-7.5 J/cm 2 , 1-3 irradiations) also had similar cell viability and stimulated cell proliferation at 72 h (20). The effect of LLLT in differentiated osteoblasts were also investigated and LLLT (685 nm, 2 J/cm 2 , 1-2 irradiations) promoted cell proliferation and viability along with expression of basic fibroblast growth factor (bFGF), insulin-like growth factor-I (IGF-I) and IGF-I (IGFBP3) receptor (21).…”

Section: Proliferation and Differentiationmentioning

confidence: 88%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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Photobiomodulation (PBM) using low-level laser therapy (LLLT) is a treatment that is increasingly used in oncology. Studies reported enhancement of wound healing with reduction in pain, tissue swelling and inflammatory conditions such as radiation dermatitis, oral mucositis, and lymphedema. However, factors such as wavelength, energy density and irradiation frequency influence the cellular mechanisms of LLLT. Moreover, the effects of LLLT vary according to cell types. Thus, controversy arose as a result of poor clinical response reported in some studies that may have used inadequately planned treatment protocols. Since LLLT may enhance tumor cell proliferation, these will also need to be considered before clinical use. This review aims to summarize the current knowledge of the cellular mechanisms of LLLT by considering its effects on cell proliferation, metabolism, angiogenesis, apoptosis and inflammation. With a better understanding of the cellular mechanisms, bridging findings from laboratory studies to clinical application can be improved.

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“…This lasting beneficial effect can be attributed to robust improvement of mitochondrial function and increased activation of secondary messenger pathways, transcription factors, and growth factor expression through PBM-induced NO and ROS signaling 24 , 74 , 93 , 95 . Although limited to cell proliferation and not for aged stem cells, two recent studies reported partially similar results by comparing single and multiple PBM treatments at two red (630 and 660 nm) wavelengths using fluences between 2 and 4 J/cm 2 96 , 97 . When rat BM-MSCs were exposed to single and multiple PBM treatments at 48-h intervals for 13 days, single treatment led to a temporary increase in cell proliferation lasting for 2 days.…”

Section: Discussionmentioning

confidence: 88%

“…In contrast, multiple treatments resulted in profound and long-term cell growth 96 , consistent with our results. In the second study, the positive effect of the multiple treatments on viability of stem cells from human exfoliated deciduous teeth was also significant compared to single treatment, but was less lasting (48 h) 97 . Overall, our results expand the findings of these studies and support the notion that the beneficial effect of PBM can be extended by applying different consecutive treatment strategies.…”

Section: Discussionmentioning

confidence: 90%

Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells

Eroglu

Genova

Zhang

et al. 2021

Sci Rep

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The plasticity and proliferative capacity of stem cells decrease with aging, compromising their tissue regenerative potential and therapeutic applications. This decline is directly linked to mitochondrial dysfunction. Here, we present an effective strategy to reverse aging of mouse bone marrow mesenchymal stem cells (BM-MSCs) by restoring their mitochondrial functionality using photobiomodulation (PBM) therapy. Following the characterization of young and aged MSCs, our results show that a near-infrared PBM treatment delivering 3 J/cm2 is the most effective modality for improving mitochondrial functionality and aging markers. Furthermore, our results unveil that young and aged MSCs respond differently to the same modality of PBM: whereas the beneficial effect of a single PBM treatment dissipates within 7 h in aged stem cells, it is lasting in young ones. Nevertheless, by applying three consecutive treatments at 24-h intervals, we were able to obtain a lasting rejuvenating effect on aged MSCs. Our findings are of particular significance for improving autologous stem cell transplantation in older individuals who need such therapies most.

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Effect of single and multiple doses of low-level laser therapy on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED)

Cited by 19 publications

References 46 publications

Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells

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