Additive Manufacturing (AM) technologies are considered revolutionary because they could fundamentally change the way products are designed. Selective Laser Melting (SLM) is a metal based AM process with significant and growing potential for the manufacture of aerospace components. Traditionally a material needs to be listed in the Metallic Materials Properties Development and Standardization (MMPDS) handbook if it is to be considered certified. However, this requires a considerable amount of test data to be generated on the materials mechanical properties. Therefore, the MMPDS certification process does not lend itself easily to the certification of AM components as the final component can have similar mechanical properties to wrought alloys combined with the defects associated with traditional casting and welding technologies. These defects can substantially decrease the fatigue life of a fabricated component. The primary purpose of this investigation was to study the fatigue behaviour of as-built Ti-6Al-4V (Ti64) samples. Fatigue tests were performed on the Ti-6Al-4V specimens built using SLM with a variety of layer thicknesses and build (vertical or horizontal) directions. Fractography revealed the presence of a range of manufacturing defects located at or near the surface of the specimens. The experimental results indicated that Lack-of-Fusion (LOF) defects were primarily responsible for fatigue crack initiation. The reduction in fatigue life appeared to be affected by the location, size and shape of the LOF defect.