Effect of size and pegylation of liposomes and peptide-based synthetic lipoproteins on tumor targeting

Tang, Jie; Kuai, Rui; Yuan, Weiying; Drake, Lindsey; Moon, James J.; Schwendeman, Anna

doi:10.1016/j.nano.2017.04.009

Cited by 51 publications

(69 citation statements)

References 56 publications

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“…Due to the negative effect of coating, PEGylation is applied as a replacement for increasing the stability and loading of the drug, as well as decreasing the size of particles. Moreover, PEG has a high biocompatibility . The current research describes the PEGylation process of characterization nanoliposomes loaded cisplatin to monitor the potency and efficacy of newly synthesized nanoliposome on the elimination of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Development of a novel liposomal nanoparticle formulation of cisplatin to breast cancer therapy

Ghafari

Haghiralsadat

Falahati-Pour

et al. 2020

J of Cellular Biochemistry

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Cisplatin is one of the conventional drugs used in chemotherapy which has a potent antitumor function. However, due to the dangerous side effects, including the damage to DNA of the normal cells, its clinical use is limited. The aim of this study was to prepare and characterize nanoliposome containing cisplatin. We optimized liposome formulations through the modification of the proportion of SPC80 (soybeanphospholipids with 75% phosphatidylcholine) and cholesterol content. Then, novel PEGylated liposomal formulations containing SPC80: cholesterol: DSPE-mPEG (at ratios of 85:10:5) were designed and developed to serve as a therapy to achieve more improved pharmaceutical efficiency. Zeta Sizer showed that PEGylated nanoliposomes had a mean diameter of 119.7 ± 2.1 nm, a zeta potential of −26.03 ± 1.34 mV, and entrapment efficiency of 96.65 ± 3%. The optimum formulations represented sustained, thermo-sensitive release, and augmented cellular uptake. The cytotoxic effect of the liposomal drug was higher than the free medication drug that confirmed the efficiency of cellular uptake. This study suggests that nanoliposome-loaded cisplatin plays a vital role in improving drug efficacy and the reduction of dosage. K E Y W O R D Sbreast cancer, chemotherapy, cisplatin, nanoliposome

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Section: Introductionmentioning

confidence: 99%

Development of a novel liposomal nanoparticle formulation of cisplatin to breast cancer therapy

Ghafari

Haghiralsadat

Falahati-Pour

et al. 2020

J of Cellular Biochemistry

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“…As SR-BI is expressed by the cells, the rHDL could -in principle -be actively taken up by all cells in the spheroids, thus resulting in likewise decreased accumulation towards the tumor center due to enhanced clearance by the outermost cells, compared to similar sized non-targeting NPs. In contrast, Tang et al 29 suggest that SR-BI receptor-mediated cell uptake of HDL-mimicking NPs in the core of the tumor spheroid is important for enhanced penetration of the NPs. They based this claim on the observation that PEGylation of the HDL-mimicking NPs decreased the penetration into the tumor spheroid, which they attribute to SR-BI interactions being diminished by PEG.…”

Section: The Effect Of Np Design On the Penetration Efficiencymentioning

confidence: 90%

“…rapid proliferation at the surface and slow metabolism -or necrosis -in the center of spheroids 25 . The majority of studies of NP distributions in tumor spheroids use tumor spheroid fixation [26][27][28][29][30][31][32][33][34] , often in combination with histological sectioning 7,[35][36][37][38] . Nevertheless, there are also reports of NP penetration in live tumor spheroids using confocal-scanning microscopy [39][40][41][42][43][44][45][46][47][48][49] .…”

Section: Introductionmentioning

confidence: 99%

Head-to-head comparison of the penetration efficiency of lipid-based nanoparticles in a 3D tumor spheroid model

Niora¹,

Pedersbæk²,

Lassen³

et al. 2020

Preprint

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<p>Most tumor-targeted drug delivery systems must overcome a large variety of physiological barriers before reaching the tumor site and diffuse through the tight network of tumor cells. Many studies focus on optimizing the first part, the accumulation of drug carriers at the tumor site, ignoring the penetration efficiency, i.e., a measure of the ability of a drug delivery system to overcome tumor surface adherence and uptake. We used 3D tumor spheroids in combination with light-sheet fluorescence microscopy in a head-to-head comparison of a variety of commonly used lipid-based nanoparticles, including liposomes, PEGylated liposomes, lipoplexes and reconstituted high-density lipoproteins (rHDL). Whilst PEGylation of liposomes only had minor effects on the penetration efficiency, we show that lipoplexes mainly associated to the periphery of tumor spheroids, possibly due to their positive surface charge leading to fusion with the cells at the spheroid surface or aggregation. Surprisingly, the rHDL showed significantly higher penetration efficiency and high accumulation inside the spheroid. While these findings indeed could be relevant when designing novel drug delivery systems based on lipid-based nanoparticles, we stress that the used platform and detailed image analysis is a versatile tool for in vitro studies of the penetration efficiency of nanoparticles in tumors.</p><div><br></div>

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“…However, as apolipoproteins are generally obtained via extraction from animal plasma or recombinant expression systems, to apply apolipoprotein‐based lipid nanodisks to the living body, some issues pertaining to their safety and productivity, including cost, need to be addressed. Instead, chemically synthesized peptides are sometimes used as scaffolding molecules for lipid nanodisks . Recently, it has been shown that styrene maleic acid copolymer (SMA), which is known to be a biocompatible synthetic polymer, can produce lipid nanodisks (SMA nanodisks).…”

Section: Introductionmentioning

confidence: 99%

Indium‐111 labeling of high‐density lipoprotein‐mimicking phospholipid‐styrene maleic acid copolymer complexes and its biodistribution in mice

Tanaka

Hosotani

Mukai

2018

Labelled Comp Radiopharmac

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Discoidal lipid nanoparticles mimicking native high-density lipoproteins (HDL) are promising delivery vehicles of drugs and/or imaging agents. However, little is known about the in vivo biodistribution of such discoidal lipid nanoparticles compared to liposomes, clinically available spherical lipid nanoparticles. Recently, it has been reported that synthetic polymers instead of apolipoproteins can be complexed with phospholipid to form discoidal nanoparticles. In the present study, with the aim of developing phospholipid-synthetic polymer complexes for future clinical applications, the biodistribution of such particles in normal mice was investigated. Lipid nanoparticles comprising 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and styrene maleic acid copolymer (SMA), having sizes similar to native HDL, were prepared using the freeze-sonication method. POPC-SMA complexes remained stable at 37°C for at least 3 days in buffer. By devising ways to avoid detrimental effects accompanied by pH reduction and nonspecific binding of In to SMA, POPC-SMA complexes were successfully labeled with In without affecting particle integrity. The biodistribution of POPC-SMA complexes in normal mice was similar to that of discoidal lipid nanoparticles composed of POPC and apolipoprotein A-I, the major protein constituent of native HDL. Unlike liposomes, the accumulation of POPC-SMA complexes in the spleen was low, suggesting that these complexes are not recognized as foreign substances. To the best of our knowledge, this is the first in vivo study of HDL-mimicking phospholipid-synthetic polymer complexes.

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Effect of size and pegylation of liposomes and peptide-based synthetic lipoproteins on tumor targeting

Cited by 51 publications

References 56 publications

Development of a novel liposomal nanoparticle formulation of cisplatin to breast cancer therapy

Development of a novel liposomal nanoparticle formulation of cisplatin to breast cancer therapy

Head-to-head comparison of the penetration efficiency of lipid-based nanoparticles in a 3D tumor spheroid model

Indium‐111 labeling of high‐density lipoprotein‐mimicking phospholipid‐styrene maleic acid copolymer complexes and its biodistribution in mice

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