Effect of smear layer deproteinization with enzyme solutions on bonding efficacy of one-step self-etch adhesives

Alshahni, Rima Zakzuk; Sato, Kento; Hosaka, Keiichi; Hatayama, Takashi; Chiba, Ayaka; Foxton, Richard M.; Tagami, Junji; Sumi, Yasunori; Shimada, Yasushi; Nakajima, Masatoshi

doi:10.1016/j.ijadhadh.2020.102672

Cited by 2 publications

(3 citation statements)

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“…These results might show that the deproteinizing effect with the ClO 2 solution extended to deeper regions of the dentin substrate. 14 Besides, ClO 2 deproteinization did not increase microlea- kage in both SE and E&R clinical techniques and did not show any alteration on the surface of the etched and non- etched deep dentin according to SEM and ATR-FTIR spectro- scopy. However, these results could not be validated as no previous reports have been in the literature about the effects of ClO 2 on the surface feature of dentin and microleakage.…”

Section: Discussionmentioning

confidence: 79%

“…This finding is similar to the results of Alshahli et al in bovine incisors and Hasija et al in primary tooth enamel. 14, 46 However, Sharefeddin et al 13 indicated that the deproteinization of smear layer–covered superficial dentin with 10% papain solution significantly increased the SBS value. In this study, deep dentin was used as the adherent.…”

Section: Discussionmentioning

confidence: 99%

“…9,11 Recently, the proteolytic enzymes, papain and bromelain, have been introduced for dental practice as deproteinizing agent. 13,14 Papain is an endoprotein similar to human pepsin (present in gastric juices), which has bactericidal, bacteriostatic, and anti-inflammatory activities. [15][16][17][18] It also partially dissolves intact unmineralized type I collagen fibrils.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Dentin Deproteinizing Agents on Bond Strength and Microleakage of Universal Adhesive to Dentin

Bedir

Telatar

2023

Journal of Advanced Oral Research

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Aim: To evaluate the effects of papain (Brix 3000), bromelain, sodium hypochlorite (NaOCl), and chlorine dioxide (ClO2) application to the deep dentin surface on shear bond strength (SBS), microleakage, and dentin surface properties. Materials and Methods: Deep dentin surface ( n = 100) for evaluating SBS, class V preparation at the buccal surface for testing microleakage ( n = 100), and deep dentin slices ( n = 20) for evaluating surface properties were conducted on the 220 molar teeth. Four different deproteinizing agents (Brix 3000, 40% bromelain, 5.25% NaOCl (Chloraxid), and 0.12% ClO2) were applied to the dentin, and then the universal adhesive was used in self-etch (SE) and etch&rinse (E&R). Deproteinizing agents were not applied to the control group. All of the samples were subjected to 5000 cycles of thermal aging at 5ºC–55ºC. SBS (MPa) was tested by a universal testing machine. The microleakage of resin composite bonded with different adhesive modes was evaluated under a stereomicroscope. The changes in the surface morphology were examined with scanning electron microscopy (SEM) and attenuated total reflection – fourier transform infrared spectroscopy (ATR-FTIR). Results: ClO2 exhibited the highest bond strength among deproteinizing agents. Compared to the SE mode, E&R mode significantly showed higher bond strength ( p < .05). In gingival margin, bromelain SE exhibited the highest marginal leakage, while Brix 3000 SE had the lowest mean microleakage score. Conclusion: Deproteinizing with ClO2 was effective in improving the SBS of universal adhesive in the E&R mode to deep dentin. Deproteinization with bromelain before universal adhesive in SE mode showed more microleakage on both the occlusal and gingival surfaces.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Dentin Deproteinizing Agents on Bond Strength and Microleakage of Universal Adhesive to Dentin

Bedir

Telatar

2023

Journal of Advanced Oral Research

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Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

Delgado

Costa

Polido

et al. 2022

Sci Rep

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Strategies aiming to improve the longevity of resin–dentin adhesive interface developed so far have only been able to retard the problem. Different approaches are thus needed. The objective of this review was to determine whether the use of collagen-depletion strategies after acid-etching procedures may improve the bond strength of resin-based materials to dentin. A systematic review was planned following 2021 PRISMA statement guidelines, with a search strategy performed in five electronic databases: PubMed/Medline, Scopus, EMBASE, SciELO and IADR Abstract Archive (last search: 17/01/2022). Inclusion criteria encompassed studies which evaluated a collagen-depletion strategy in acid-etched human dentin and tensile/shear bond strength tests. Risk of bias assessment was carried out by two reviewers, working independently on an adapted five-domain risk of bias (RoB) checklist for laboratory studies. Results were synthesized qualitatively, as a meta-analysis was not possible due to limited number of studies and their RoB. A total of eight studies were eligible for inclusion in the systematic review after inclusion/exclusion criteria application. Out of these, two evaluated the effect of using NaOCl followed by an antioxidant, and the remaining six evaluated different enzymatic treatments (bromelain, chondroitinase ABC, papain, and trypsin). None of the studies reported a decrease of bond strength when a collagen-depletion strategy was used, in comparison to traditional hybrid layers (control). All enzymatic treatment studies which respected the inclusion criteria improved the bond strength to dentin. Some specific collagen-depletion strategies seem to play a favorable role in improving immediate bond strengths to dentin. Further research with sound methodology is required to consolidate these findings, since limitations in RoB and a low number of studies were found. The assessment of further proteolytic agents and long-term outcomes is also required.

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Effect of smear layer deproteinization with enzyme solutions on bonding efficacy of one-step self-etch adhesives

Cited by 2 publications

References 23 publications

Comparison of Different Dentin Deproteinizing Agents on Bond Strength and Microleakage of Universal Adhesive to Dentin

Comparison of Different Dentin Deproteinizing Agents on Bond Strength and Microleakage of Universal Adhesive to Dentin

Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

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