Effect of snail mucus on angiogenesis during wound healing

Abstract

Background: Angiogenesis is the process through which new blood vessels are formed from existing ones. This process plays an important role in supplying the oxygen and nutrients needed for cellular metabolism and eliminating cell debris during wound healing. Snail mucus can bind to several factors that stimulate angiogenesis, including vascular endothelial growth factor, platelet-derived growth factor, and fibroblast growth factor. The aim of this study is to observe changes in angiogenesis during the healing of wounds topically applied with snail mucus. Methods: Punch biopsy was performed on the back of male Wistar rats to obtain four wounds, and different concentrations of snail mucus were applied to each of these wounds. The animals were sacrificed on days 2, 4, and 7 to observe the extent of angiogenesis during wound healing by microscopy. Results: Two-way ANOVA showed differences in number of blood vessels formed (p = 0.00) and day of observation (p = 0.00) between groups. Post hoc Tukey’s HSD test showed that 24% snail mucus treatment does not significantly affect wound healing (p = 0.488); by contrast, treatment with 48% and 96% snail mucus demonstrated significant effects on angiogenesis (p = 0.01). Spearman’s test showed interactive effects between snail mucus concentration and day of observation on the extent of angiogenesis (p = 0.001, R = 0.946). Conclusion: Topical application of snail mucus gel can increase angiogenesis during wound healing in Wistar rat skin.

Silver/Snail Mucous PVA Nanofibers: Electrospun Synthesis and Antibacterial and Wound Healing Activities

“…Snail mucus is an attractive natural substance that is increasingly used in cosmetic creams due to its emollient, moisturizing, protective, and reparative properties. Rosanto et al [ 5 ] investigated the wound healing effect upon using different concentrations of snail mucus after the daily application of SM gel. It was concluded that topical application of 48% and 96% snail mucus gel increased the angiogenesis during the wound healing process in Wistar rat skin.…”

Acceleration of angiogenesis in wound healing after tooth extraction with kirinyuh (Chromolaena odorata) leaf extract

“…The process of stabilization and maturation of new blood vessels that is formed is also influenced by the presence of pericite cells in the walls of the blood vessels. A decrease in angiogenesis occurs due to selective apoptotic processes of new blood vessels that do not have pericits [4,18,19]. This raises the possibility that the 10% kirinyuh leaf extract gel also plays a decrease in the end of the proliferation phase on day 7.…”

“…Wounds cause loss of tissue continuity that causes the wound area to be deprived of oxygen and nutrients that are essential for healing [3]. Angiogenesis is an important step in the wound healing process because it will overcome the condition [4]. In general, angiogenesis is divided into three phases: initiation, invasion and maturation.…”

“…The maturation process will cause selective apoptosis which causes a reduction in the * Corresponding author: yosaphatbr@ugm.ac.id number of new blood vessels to reach the ideal number. Therefore, the angiogenesis process can be assessed by counting the number of blood vessels formed in the wound [4,5].…”