Effect of sodium butyrate on intestinal inflammatory response to lipopolysaccharide in broiler chickens

Abstract

G. 2015. Effect of sodium butyrate on intestinal inflammatory response to lipopolysaccharide in broiler chickens. Can. J. Anim. Sci. 95: 389Á395. The aim of this study was to investigate the effect of sodium butyrate (SB) supplementation on intestinal inflammatory response to lipopolysaccharide (LPS) in broiler chickens. A total of 120 one-day-old chickens (Arbor Acres) were divided into two groups and fed a control diet (without SB) or 1.00 g SB kg(1 diet. Half of the chickens fed each diet were injected intra-peritoneally with 0.5 g kg (1 body weight of Escherichia coli LPS at 16, 18 and 20 d of age. The results showed that the LPS challenge decreased (PB0.05) villus height and the ratio of villus height to crypt depth (V/C ratio), increased (PB0.01) crypt depth of the duodenum. SB supplementation increased (P B0.05) villus height, crypt depth and V/C ratio of the duodenum and jejunum, except for the crypt depth of the jejunum. The LPS challenge increased (P B0.05) myeloperoxidase (MPO) activities, intercellular adhesion molecule-1 (ICAM-1) and activated nuclear factor kappa B (NF-kB) levels in mucosa of the duodenum and jejunum, while decreasing (PB0.05) insulin-like growth factor-1 (IGF-1) concentrations. The LPS challenge increased (PB0.05) the mRNA levels of interleukin-1beta (IL-1b), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-a) in duodenal mucosa. SB supplementation decreased (P B0.05) the MPO activities and mRNA expression of TNF-a in the duodenal mucosa, and the activated NF-kB levels in mucosa of the duodenum and jejunum. There were no significant interactions between dietary SB and LPS on the histomorphology of the small intestine and those inflammatory mediators except for MPO and ICAM-1 in duodenal mucosa (P B0.05). The results indicate that SB supplementation could improve the intestinal morphology and function of broiler chickens and partially attenuate inflammatory responses caused by LPS challenge but not involving NF-kB activation.Key words: Sodium butyrate, intestinal inflammatory response, lipopolysaccharide, broiler chicken Jiang, Y., Zhang, W., Gao, F. et Zhou, G. 2015. Les effets du butyrate de sodium sur la re´ponse inflammatoire intestinale au lipopolysaccharide chez les poulets a`griller. Can. J. Anim. Sci. 95: 389Á395. Le but de cette e´tude e´tait d'examiner les effets de l'ajout du butyrate de sodium (SB * « sodium butyrate ») sur la re´ponse inflammatoire intestinale au lipopolysaccharide (LPS) chez les poulets a`griller. Un total de 120 poulets (Arbor Acres) aˆge´s d'un jour ont e´te´divise´s en deux groupes et nourris d'une die`te te´moin (sans SB) ou avec 1,00 g SB kg (1 . La moitie´des poulets nourris de chaque die`te ont rec¸u une injection intra-pe´ritone´ale de 0,5 g kg (1 de poids corporel de LPS d'Escherichia coli a`l'aˆge de 16, 18 et 20 jours. Les re´sultats de´montraient que l'infection au LPS diminuait (PB0,05) la hauteur des villosite´s et le ratio de hauteur de villosite´a`profondeurs de cryptes (ratio V/C) et augmentait (PB0,01) la profondeur des cry...

Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers

“…Immune stress is often associated with the inflammation response, and cytokines released in the inflammation response could promote metabolism and suppress anabolism of carbohydrate, protein and fat to inhibit growth of animals ( Klasing, 1988 , Johnson, 1997 , Webel et al., 1997 , Sijben et al., 2001 , Hwang et al., 2008 ). Pro-inflammatory cytokines such as IL-1β, IL-6 and tumor necrosis factor-α (TNF-α) originated from macrophages were the major cellular messengers initiating metabolic cascade alterations following the innate immune response ( Jiang et al., 2015 ). Lipopolysaccharide was demonstrated to increase pro-inflammatory cytokines secretion and suppress animal growth ( Takahashi, 2012 , Tan et al., 2014 , Li et al., 2015b ).…”

Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil

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Effects of live yeast on immune responses and intestinal morphological structure in lipopolysaccharide (LPS)-challenged broilers

“…It had been used to imitate microbial stress in broilers (Korver and Klasing 1997). Previous studies in broilers revealed that LPS challenge compromised growth performance (Korver and Klasing 1997;Li et al 2015), impaired both innate and adaptive immunity (Korver and Klasing 1997), and induced intestinal inflammation coupled with a damage of intestinal structure and barrier function as evidenced by the destructive intestinal morphology and tight junctions (Hu et al 2011;Jiang et al 2015;Li et al 2015). Yeast oligosaccharide had been shown to improve performance (Morales-Lopez and Brufau 2013) and to modulate systemic and intestinal immune responses in LPS-challenged broilers (Morales-Lopez and Brufau 2013; Shanmugasundaram et al 2014).…”