Effect of soft foods on primary human gingival epithelial cell growth and the wound healing process

Rouabhia, Mahmoud; Rouabhia, Dounia; Park, Hyun Jin; Giasson, Luc; Zhang, Ze

doi:10.1016/j.foodres.2017.07.041

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…The phenomenon of cell migration in tissue organisms has a twofold significance. It is necessary for the wound healing process [ 2 , 3 ] but it also contributes to the increase in motility and invasion of cancer cells [ 4 ]. Metastasis accounts for over 90% of cancer-related mortality, highlighting the need to increase the understanding of the regulatory mechanisms responsible for cell motility and targeting cancer cell migration is of great importance.…”

Section: Introductionmentioning

confidence: 99%

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

2018

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Actin functions are crucial for the ability of the cell to execute dynamic cytoskeleton reorganization and movement. Nutraceuticals that form complexes with actin and reduce its polymerization can be used in cancer therapy to prevent cell migration and metastasis of tumors. The aim of this study was to evaluate the ability of isoflavones to form complexes with actin. Docking simulation and isothermal titration calorimetry were used for this purpose. The formation of complexes by hydrogen bonds, hydrophobic and π-π interactions was demonstrated. Interactions occurred at the ATP binding site, which may limit the rotation of the actin molecule observed during polymerization and also at the site responsible for contacts during polymerization, reducing the ability of the molecule to form filaments. The greatest therapeutic potential was demonstrated by isoflavones occurring in red clover sprouts, i.e., biochanin A and formononetin, being methoxy derivatives of genistein and daidzein.

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Section: Introductionmentioning

confidence: 99%

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

2018

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“…In an additional in vitro study into the post-wound interactions between gingival epithelial cells and drinkable yogurt, the authors observed a positive effect on oral wound healing. This was explained by an increase in various keratins and in β-defensin-2 production [65]. These keratins are important for the development of epidermal structures [66].…”

Section: Discussionmentioning

confidence: 99%

“…These keratins are important for the development of epidermal structures [66]. β-defensin stimulates cell migration and angiogenesis at wound sites and promotes wound closure [38,65].…”

Section: Discussionmentioning

confidence: 99%

Avoidance of milk and dairy products after oral surgery—is such a recommendation still valid? A cross-sectional study among German and international oral and maxillofacial surgeons and dental practitioners with review of the literature

Moghaddam

Neff

2021

Oral Maxillofac Surg

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Purpose For prevention of wound-healing complications, patients in German-speaking countries are traditionally advised to avoid consumption of milk and dairy products after oral surgery. In the absence of national and international guidelines, this study investigates scientific evidence and compares international practice, frequency scale, and rationale behind such recommendation. Methods Comparison of a German cross-sectional mono-center-questionnaire pilot study and a survey among international oral and maxillofacial surgeons (OMFS), specialized oral surgeons and general dentists, evaluating international practice regarding post-operative dietary and nutrition recommendations. Our literature review further assessed scientific evidence for relevant effects of probiotics, prebiotics, and/or synbiotics. Results Among German study participants, 56% (n = 64/114) advise patients to avoid milk and dairy products, with 42% of OMFS (n = 38) and 65% (n = 76) of the general dentists recommending abstention (p = .027). In striking contrast, such recommendation could not be identified in our international survey (n = 143) (t test, p < .001) nor in the literature. There were significant differences between German and international study participants regarding the rationale for dietary recommendations, with dental schools and literature most frequently indicated as sources (Fisher’s exact test, p < .001). Conclusion The hypothesis of a harmful effect of the consumption of milk and dairy products after dentoalveolar surgery could not be supported by evidence. The recommendation to avoid dairy products post-surgery was identified as a specific phenomenon practiced almost exclusively in German-speaking countries. Corresponding recommendations, most probably based on a now irrelevant risk of contracting tuberculosis from milk products, can at present no longer be substantiated.

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“…The method of cell culture and 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) test were conducted as described previously (Rouabhia et al, 2017). Briefly, Hela and Vero cells were grown in 90% Dulbecco's modified Eagle's medium (DMEM)-high glucose culture medium (Gibco) supplemented with 10% FBS (Gibco) and 1% penicillinstreptomycin (Gibco), and seeded on a 96-well microtiter plate at a density of 2 × 10 4 cells per well.…”

Section: Cytotoxicitymentioning

confidence: 99%

A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance

Ning

Xue

et al. 2021

Front. Microbiol.

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Human beta-defensins (hBDs) play an important role in the host defense against various microbes, showing different levels of antibacterial activity and salt resistance in vitro. It is of interest to investigate whether can chimeric hBD analogs enhanced antibacterial activity and salt resistance. In this study, we designed a chimeric human defensin, named H4, by combining sequences of human beta-defensin-3 (hBD-3) and human beta-defensin-4 (hBD-4), then evaluated its antibacterial activity, salt resistance, and cytotoxic effects. The result showed that the antibacterial activity of H4 against most tested strains, including Klebsiella pneumonia, Enterococcus faecalis, Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Acinetobacter baumannii was significantly improved compared to that of hBD-3 and hBD-4. Notably, H4 exhibited significantly better antibacterial activity against multidrug resistant isolate A. baumannii MDR-ZJ06 than commonly used antibiotics. Chimeric H4 still showed more than 80% antibacterial activity at high salt concentration (150 μM), which proves its good salt tolerance. The cytotoxic effect assay showed that the toxicity of H4 to Hela, Vero, A549 cells and erythrocytes at a low dose (<10 μg/ml) was similar to that of hBD-3 and hBD-4. In conclusion, given its broad spectrum of antibacterial activity and high salt resistance, chimeric H4 could serve as a promising template for new therapeutic antimicrobial agents.

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Effect of soft foods on primary human gingival epithelial cell growth and the wound healing process

Cited by 5 publications

References 38 publications

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells

Avoidance of milk and dairy products after oral surgery—is such a recommendation still valid? A cross-sectional study among German and international oral and maxillofacial surgeons and dental practitioners with review of the literature

A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance

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