Effect of solid-medium coupled with reactive oxygen species on ganoderic acid biosynthesis and MAP kinase phosphorylation in Ganoderma lucidum

You, Bang‐Jau; Chang, Wei-Chieh; Chung, Kwong T.; Kuo, Yueh‐Hsiung; Yang, Chang-Syun; Tien, Ni; Hsieh, Hui-Chuan; Lai, Chun-Chien; Lee, Hong‐Zin

doi:10.1016/j.foodres.2012.09.003

Cited by 23 publications

(30 citation statements)

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“…However, in this study, aspirin induced GAs production but reduced transcript of the LS and SQS. Our previous study has shown that high doses of ROS, which induce GAs biosynthesis, also reduce SQS and LS mRNA expression [19]. These findings support the idea that aspirin, as well as high doses of ROS, may up-regulate the GA biosynthetic genes down-stream of lanosterol biosynthesis [19].…”

Section: Resultssupporting

confidence: 56%

“…Our previous study has shown that high doses of ROS, which induce GAs biosynthesis, also reduce SQS and LS mRNA expression [19]. These findings support the idea that aspirin, as well as high doses of ROS, may up-regulate the GA biosynthetic genes down-stream of lanosterol biosynthesis [19]. However, the role of HMGR in apoptosis-induced GA biosynthesis is unknown.…”

Section: Resultsmentioning

confidence: 59%

“…However, whether abiotic stress induces secondary metabolites biosynthesis during cell apoptosis remains unknown. Recently studies have indicated that methyl jasmonate and ROS, which were previously used to enhance plants secondary metabolites production [38], also increased GA biosynthesis in G. lucidum [16], [19], [20]. These findings suggest that one or more common regulatory components may control secondary metabolite biosynthesis in fungi and plants.…”

Section: Resultsmentioning

confidence: 86%

“…However, incubating G. lucidum with low doses of H 2 O 2 and CDNB has no effect on GA and biomass production [19]. These results suggest that cell death is related to GA production in G. lucidum .…”

Section: Introductionmentioning

confidence: 78%

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A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction

et al. 2013

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Ganoderma lucidum is one of most widely used herbal medicine and functional food in Asia, and ganoderic acids (GAs) are its active ingredients. Regulation of GA biosynthesis and enhancing GA production are critical to using G. lucidum as a medicine. However, regulation of GA biosynthesis by various signaling remains poorly understood. This study investigated the role of apoptosis signaling on GA biosynthesis and presented a novel approach, namely apoptosis induction, to increasing GA production. Aspirin was able to induce cell apoptosis in G. lucidum, which was identified by terminal deoxynucleotidyl transferase mediated dUPT nick end labeling assay positive staining and a condensed nuclear morphology. The maximum induction of lanosta-7,9(11), 24-trien-3α-01-26-oic acid (ganoderic acid 24, GA24) production and total GA production by aspirin were 2.7-fold and 2.8-fold, respectively, after 1 day. Significantly lower levels of GA 24 and total GAs were obtained after regular fungal culture for 1.5 months. ROS accumulation and phosphorylation of Hog-1 kinase, a putative homolog of MAPK p38 in mammals, occurred after aspirin treatment indicating that both factors may be involved in GA biosynthetic regulation. However, aspirin also reduced expression of the squalene synthase and lanosterol synthase coding genes, suggesting that these genes are not critical for GA induction. To the best of our knowledge, this is the first report showing that GA biosynthesis is linked to fungal apoptosis and provides a new approach to enhancing secondary metabolite production in fungi.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 78%

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A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction

et al. 2013

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“…40(157):600-607, octubre-diciembre de 2016 doi: http://dx.doi.org/10.18257/raccefyn.391 como medio de cultivo (sustrato), son algunas de las bondades que ofrece el cultivo de las especies fúngicas (Suarez & Nieto, 2013). Las rutas metabólicas de los hongos están determinadas por el sustrato de fijación, así hongos de una misma especie, desarrollados en diferentes sustratos o distintos lugares pueden variar en su composición química, aspectos relevantes para las empresas que incursionan en el cultivo de hongos con fines alimenticios y farmacéuticos (González, et al, 1999;You, et al, 2012).…”

Section: Introductionunclassified

Estudio químico de la fracción de acetato de etilo del hongo Ganoderma lucidum cultivado en desechos agroindustriales

Ordosgoitia

Martínez

Chavez

et al. 2016

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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ResumenEstudios en hongos han revelado que poseen un alto valor nutricional, comprobada acción farmacológica de los metabolitos secundarios producidos en sus cuerpos fructíferos, siendo muchas las especies estudiadas, destacando entre estas el hongo macromiceto Ganoderma lucidum, del cual se han aislado compuestos tipo terpenoides y sus derivados, de importancia biológica, mostrando efectividad en la disminución de la presión sanguínea, propiedades antinflamatorias, antivirales, hipoglicémicas e inmunoprotectoras. Por otro lado, el cultivo de hongos promueve la solución a problemas de tipo ambiental, ya que puede hacerse en desechos agroindustriales. El objetivo fue estudiar la composición química del hongo G. lucidum, cultivado en desechos agroindustriales y determinar diferencias entre los metabolitos obtenidos, con respecto a los de la especie del hongo en su estado silvestre. Para ello, se cultivó G. lucidum, en desechos agroindustriales y se estudió químicamente la fracción de acetato de etilo (AcOEt), mediante cromatografía de columna, cromatografía de capa fina, cromatografía en capa fina preparativa. Además de técnicas de análisis de estructuras CG-EM, RMN-1 H, IR. Los resultados indicaron que la separación cromatografía, permitió la caracterización de 8 compuestos. De forma general, se puede concluir que el hongo cultivado, presenta una composición de metabolitos secundarios similar a la reportada en distintas investigaciones sobre la especie en su estado silvestre, estableciéndose que el compuesto mayoritario corresponde al ganoderiol B. © 2016. Acad. Colomb. Cienc. Ex. Fis. Nat. Palabras clave: Ganoderma lucidum; Cultivo; Estudio químico; Metabolitos secundarios.Chemical study of fraction ethyl acetate in the fungus Ganoderma lucidum, grown in agro-industrial waste. AbstractStudies in fungi have revealed that they have a high nutritional value, proven pharmacological action of the secondary metabolites produced in their fruiting bodies, being many species studied, emphasizing among these, the macromycete Ganoderma lucidum, from which have been isolated compounds type terpenoids and its derivatives, with biological significance, showing effectiveness in the lowering blood pressure, anti-inflammatory properties, antiviral, hypoglycemic and immunoprotective. On the other hand, mushroom cultivation promotes the solution to environmental problems, since it can be done in agro-industrial wastes. The objective was to study the chemical composition of the fungus G. lucidum, grown in agro-industrial wastes and to determine differences between the metabolites obtained, with respect to those of the species of the fungus in their wild state. To do so, G. lucidum, was cultivated in agro-industrial wastes and the ethyl acetate fraction was studied, throught chromatography column thin layer chromatography, preparative thin-layer chromatography. In addition, GC-MS, NMR-1 H, IR analysis techniques were done. The results and preparative indicated that the chromatographic separation allowed the characterization of 8 com...

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Effects of different graphene‐based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum

Rostami

Yazdian

Mirjani

et al. 2020

Biotechnology Progress

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Graphene-based nanomaterials (GBNs) have attracted considerable interest nowadays due to their wide range of applications. However, very little attention has been paid to the application of nanomaterials as potential elicitors for production of valuable metabolites. Herein, aiming to earn insight into effects of nanomaterials on secondary metabolite biosynthesis by medicinal fungi, we evaluated the influence of GBNs on growth and production of ganoderic acid (GA) by Ganoderma lucidum in submerged culture. Graphene oxide (GO), reduced graphene oxide (rGO), and rGO/Fe 3 O 4 nanocomposite were synthesized successfully and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy analysis. The prepared nanomaterials were added to the culture of G. lucidum at final concentrations of 50, 100, and 150 mg/L on Day 5. The results showed that the elicitation of G. lucidum with GO and rGO decreased the cell dry weight and GA production slightly, especially in higher concentrations. However, rGO/Fe 3 O 4 nanocomposite not negatively affected cell growth and improved GA production. G. lucidum growth rate responded to elicitation experiments differently and depended on the type of nanomaterials and their concentrations, but almost all GBNs caused an increase in GA content (mg/100 mg dry weight). Also, field emission scanning electron microscopy morphological study showed that under elicitation, mycelia were more condensed and tightly stacked together. The findings from this study may suggest that GBNs in low concentrations could be applied as elicitors to secondary metabolites production from higher fungus, but further environmental, physiological, and biological studies required.

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Effect of solid-medium coupled with reactive oxygen species on ganoderic acid biosynthesis and MAP kinase phosphorylation in Ganoderma lucidum

Cited by 23 publications

References 54 publications

A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction

A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Induction

Estudio químico de la fracción de acetato de etilo del hongo Ganoderma lucidum cultivado en desechos agroindustriales

Effects of different graphene‐based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum

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