Effect of solvents on inhibition of collagen-bound proteases by chlorhexidine

Ekambaram, Manikandan; Epasinghe, Don Jeevanie; Yiu, Cynthia Kar Yung; Matinlinna, Jukka Pekka

doi:10.1016/j.ijadhadh.2015.03.010

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…After this, 2037 articles were excluded because they did not meet the inclusion criteria, leaving a total of 110 articles to be evaluated by full-text reading. Of these articles, 60 were not considered in the qualitative analysis: 1 article was in a language other than English [ 20 ], 2 studies were clinical trials [ 21 , 22 ], 1 study evaluated the BS only to carious dentin [ 23 ], 3 articles used the crosslinker agent within the composition of the phosphoric acid [ 24 , 25 , 26 ], 13 studies evaluated experimental adhesives [ 12 , 14 , 16 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ], for 5 articles we could not retrieve the full text [ 37 , 38 , 39 , 40 , 41 ], 25 articles did not evaluate any BS test [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ], 8 articles did not use a crosslinker agent [ ...…”

Section: Resultsmentioning

confidence: 99%

Effect of Collagen Crosslinkers on Dentin Bond Strength of Adhesive Systems: A Systematic Review and Meta-Analysis

Hardan

Daood

Bourgi

et al. 2022

Cells

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This study aimed to identify the role of crosslinking agents in the resin–dentin bond strength (BS) when used as modifiers in adhesives or pretreatments to the dentin surface through a systematic review and meta-analysis. This paper was conducted according to the directions of the PRISMA 2020 statement. The research question of this review was: “Would the use of crosslinkers agents improve the BS of resin-based materials to dentin?” The literature search was conducted in the following databases: Embase, PubMed, Scielo, Scopus, and Web of Science. Manuscripts that reported the effect on the BS after the use of crosslinking agents were included. The meta-analyses were performed using Review Manager v5.4.1. The comparisons were performed by comparing the standardized mean difference between the BS values obtained using the crosslinker agent or the control group. The subgroup comparisons were performed based on the adhesive strategy used (total-etch or self-etch). The immediate and long-term data were analyzed separately. A total of 50 articles were included in the qualitative analysis, while 45 articles were considered for the quantitative analysis. The meta-analysis suggested that pretreatment with epigallocatechin-3-gallate (EGCG), carbodiimide, ethylenediaminetetraacetic acid (EDTA), glutaraldehyde, and riboflavin crosslinking agents improved the long-term BS of resin composites to dentin (p £ 0.02). On the other hand, the use of proanthocyanidins as a pretreatment improved both the immediate and long-term BS values (p ≤ 0.02). When incorporated within the adhesive formulation, only glutaraldehyde, riboflavin, and EGCG improved the long-term BS to dentin. It could be concluded that the application of different crosslinking agents such as carbodiimide, EDTA, glutaraldehyde, riboflavin, and EGCG improved the long-term BS of adhesive systems to dentin. This effect was observed when these crosslinkers were used as a separate step and when incorporated within the formulation of the adhesive system.

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Section: Resultsmentioning

confidence: 99%

Effect of Collagen Crosslinkers on Dentin Bond Strength of Adhesive Systems: A Systematic Review and Meta-Analysis

Hardan

Daood

Bourgi

et al. 2022

Cells

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“…Hence, beyond the introduction of extensive crosslinks which could sterically block the active sites on MMPs, both TF and CR can also directly inhibit MMPs and protect dentin collagen from degradation [ 9 , 55 ]. Ability of EDC/NHS to form inter- and intrahelical bonds allows it to crosslink dentin collagen as well as the dentin-matrix-bound MMPs [ 56 ]. The carboxyl and amino groups in MMPs are more accessible than those in collagen, making EDC/NHS a potent MMP inhibitor than a collagen crosslinker [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Crosslinkers on Denatured Dentin Collagen’s Biostability, MMP Inhibition and Mechanical Properties

Nisar,

Hass,

Wang

et al. 2023

Polymers

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Objective: Sound, natural dentin collagen can be stabilized against enzymatic degradation through exogenous crosslinking treatment for durable bonding; however, the effect on denatured dentin (DD) collagen is unknown. Hence, the ability of different crosslinkers to enhance/restore the properties of DD collagen was assessed. Methods: Demineralized natural and DD collagen films (7 mm × 7 mm × 7 µm) and beams (0.8 mm × 0.8 mm × 7 mm) were prepared. DD collagen was experimentally produced by heat or acid exposure, which was then assessed by various techniques. All specimens were then treated with 1 wt% of chemical crosslinker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/n-hydroxysuccinimide (EDC/NHS) and two structurally different flavonoids—theaflavins (TF) from black tea and type-A proanthocyanidins from cranberry juice (CR) for either 30 s or 1 h. The controls were untreated. Dentin films were assessed for chemical interaction and cross-linking effect by FTIR, biostability against exogenous collagenase by weight loss (WL) and hydroxyproline release (HYP), and endogenous matrix metalloproteinases (MMPs) activity by confocal laser microscopy. Dentin beams were evaluated for tensile properties. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: Compared with natural collagen, DD collagen showed pronounced structural changes, altered biostability and decreased mechanical properties, which were then improved to various degrees that were dependent on the crosslinkers used, with EDC/NHS being the least effective. Surprisingly, the well-known MMP inhibitor EDC/NHS showed negligible effect on or even increased MMP activity in DD collagen. As compared with control, cross-linking induced by TF and CR significantly increased collagen biostability (reduced WL and HYP release, p < 0.05), MMP inhibition (p < 0.001) and mechanical properties (p < 0.05), regardless of denaturation. Conclusions: DD collagen cannot or can only minimally be stabilized via EDC/NHS crosslinking; however, the challenging substrate of DD collagen can be enhanced or restored using the promising flavonoids TF and CR.

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Effect of solvents on inhibition of collagen-bound proteases by chlorhexidine

Cited by 2 publications

References 41 publications

Effect of Collagen Crosslinkers on Dentin Bond Strength of Adhesive Systems: A Systematic Review and Meta-Analysis

Effect of Collagen Crosslinkers on Dentin Bond Strength of Adhesive Systems: A Systematic Review and Meta-Analysis

Effect of Different Crosslinkers on Denatured Dentin Collagen’s Biostability, MMP Inhibition and Mechanical Properties

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