Effect of some Chemical Inducers on Antagonistic Potential of Trichoderma harzianum against Rhizoctonia solani and it's Metabolites Production

Abstract

The present study was conducted on different supplemented gliotoxin growth medium by using some chemical inducers (micronutrients mixture, potassium tartrate and thiamine) to improve antagonism, crude extracts and volatile compounds activities of T. harzianum against R. solani. T. harzianum grown on medium supplemented with potassium tartrate decreased the mycelial growth of R. solani by 89% compared with T. harzianum alone (63.3%). Also, exhibited the significantly higher activity of the crude extract and the volatile compounds. The micronutrients followed by the thiamine addition recorded the highest activity of the secondary metabolite. Treated Trichoderma enhanced bioactivity against the pathogen when transferred on PDA medium without addition of the tested materials. Light microscope observation revealed that malformed and damaged pathogen hyphae occurred more clearly after contact with the treat ed T. harzianum. The GC-MS analysis showed detection of antifungal compounds as 2,3-butanediol, Decane, 2,4,6-trimethyl-, phenylethyl alcohol, acetic acid,

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

“…Among active constituents detected in the current fungal filtrate, phenol, 2-methyl-5-(1-methylethyl)-, or carvacrol was found to be the major unique volatile compounds in the tested extract (65.04%), which was, recently, demonstrated as among the main active ingredients of T. harzianum filtrate with significant antifungal activity against the phytopathogenic Rhizoctonia solani (Yousef et al, 2018). Similarly, phenol 2,4, bis (1,1-dimethyl ethyl) was reported as a main secondary metabolite in the filtrates of T. harzianum and T. asperellum that prompted the germination and the growth of the tomato seedling (Yuef et al, 2018).…”