Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)

Shahat, Mohamed; El-Sheikh, Tarek M.Y.; Hammad, Kotb M.; Hasaballah, Ahmed I.; Shehata, Ahmed Z.I.

doi:10.21608/eajbse.2020.128498

Cited by 4 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results agree with those of Zayed et al (2019), who showed a significant increase in GST activity in Cx. pipiens larvae and adults in response to a temperature raised from 20 to 30 C. Similar results were also observed by Shahat et al (2020), Sengodan et al (2020) and Prakash et al (2021) when evaluated the effect of Origanum syriacum, Rhizophora mucronata and Decalepis hamiltonii extracts against mosquito species and observed disturbance in GST activity.…”

Section: Table (4)supporting

confidence: 82%

Influence of temperature on the efficiency of Commiphora molmol and Acetylsalicylic acid against Culex pipiens (Diptera: Culicidae)

El-Dahab

Baz

El-Sayed

et al. 2023

Catrina: The International Journal of Environmental Sciences

View full text Add to dashboard Cite

The most important factor influencing the growth and survival of mosquito larvae is temperature. As a result, changes in the surrounding temperature may have an effect on the chemicals' toxicity to mosquitoes. The goal of the current study was to determine how temperature affected Culex pipiens' reactions to Commiphora molmol resin, Mirazid, crystalline (CASA), and acetylsalicylic acid in pharmaceutical form (PASA). Bioassay tests were carried out on the 3 rd instar larvae under laboratory conditions, and the LC 50 of each compound was determined. The LC 50 of the four compounds were used to study the temperature-toxicity relationship, while the LC 50 of Mirazid and (PASA), as the most toxic compounds, were used for further biochemical studies. Results indicated that, Mirazid was the most toxic compound, with LC 50 of 28.3 ppm followed by C. molmol resin with LC 50 of 41.6 ppm while the CASA was the least toxic compound with LC 50 of 799.7 ppm. The temperature has a considerable impact on Cx. pipiens larval susceptibility to the tested compounds. High temperature (36 C) resulted in high mortality rate (72.88, 78.20, 58.8 and 62.12 % for of C. molmol resin, Mirazid, CASA and PASA, respectively. Moreover, there were significant changes in α-esterase, β-esterase and glutathione -S-transferase levels as well as the total protein content in the treated larvae due to the change in temperature. The efficacy of Mirazid and C. molmol resin under wide range of temperatures made these compounds suitable candidates for controlling Cx. pipiens.

show abstract

Section: Table (4)supporting

confidence: 82%

Influence of temperature on the efficiency of Commiphora molmol and Acetylsalicylic acid against Culex pipiens (Diptera: Culicidae)

El-Dahab

Baz

El-Sayed

et al. 2023

Catrina: The International Journal of Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…Based on obtained results of Shahat et al (2020), methanol, ethyl acetate, chlorobenzene, and hexane extracts from leaves of Origanum syriacum, Pergularia tomentosa, Senna italica , and Otostegia fruticose decreased the total carbohydrate, protein, and lipid contents against Cx. pipiens (Shahat et al, 2020). Likewise, feeding Agrotis ipsilon larvae on Conyza aegyptiaca, Melia azedarach , and Vinca rosae induced a significant reduction in the total protein, lipids, and carbohydrates (Ramadan, 2020).…”

Section: Discussionmentioning

confidence: 93%

“…Also, Citrus limonum essential oil caused in Sitophilus granarius a decline of lipid and carbohydrate contents (Guettal et al, 2020). Based on obtained results of Shahat et al (2020), methanol, ethyl acetate, chlorobenzene, and hexane extracts from leaves of Origanum syriacum, Pergularia tomentosa, Senna italica, and Otostegia fruticose decreased the total carbohydrate, protein, and lipid contents against Cx. pipiens (Shahat et al, 2020).…”

Section: Determination Of Metabolic Profilementioning

confidence: 99%

Section: Determination Of Metabolic Profilementioning

confidence: 99%

See 1 more Smart Citation

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Hafsi

Hamaidia

Soltani

2022

Physiological Entomology

View full text Add to dashboard Cite

To select potential plant‐based insecticides, Tecoma stans (Bignoniaceae) leaf extract was screened for its larvicidal and delayed effects against a medically important mosquito species Culex pipiens L. (Diptera: Culicidae). First, gas chromatography–mass spectrometry (GC–MS) was conducted on T. stans extract, collected in ethanol for its chemical characterization and detection of active constituents. Second, insecticidal bioassays were made with several concentrations on earlier fourth instar larvae (L4) of Cx. pipiens for 24 h as recommended by WHO, in order to determine the lethality parameters of the tested extract. For that, two concentrations (LC30 and LC50) were applied on L4 for 24 h, and emerged adults were observed for their reproductive performance success like fecundity, percentage of hatching (fertility), body and gonads' volume. The biochemical composition of whole bodies of adults was investigated. Also, the specific activities of acetylcholinesterase (AChE) and glutathion S‐transferase (GST), biomarkers of neurotoxicity and detoxification, respectively, were also determined in L4 at different time intervals. Qualitative and quantitative phytochemical analysis (GC–MS) of the extract unveiled nine phytoconstituents with 2‐[4‐cyclohexylbutanoylamino]‐3‐chloro‐1,4‐naphthoquinone (36.24%) and 1,2‐Benzenedicarboxylic acid, mono(2‐ethylhexyl) ester (22.65%) as major components. The early L4 of Cx. pipiens were exposed to LC30 and LC50, and after 24 h, surviving individuals have been further reared until adult emergence. All over, T. stans leaves ethanolic extract impairs adults reproductive traits; it adversely affected gonad's size, fecundity and fertility, which negatively affected the survival of their offspring. Lipids contents were found to increase in both male and female adults. T. stans ethanolic extract increased GST activity and slightly inhibited AChE activity in L4. In conclusion, the ethanolic extract of T. stans could be a potential candidate used as a sustainable botanical insecticide for controlling mosquito.

show abstract

“…A similar study by Shahat et al (2020) reported that extracts of Origanum syriacum, Pergularia tomentosa, Senna italica, and Otostegia fruticose significantly decreased AChE activity of 3 rd instar larvae of Cx. pipiens.…”

Section: Discussionmentioning

confidence: 56%

Phytochemical composition of almond oil from Melia azedarach L. and its larvicidal, ovicidal, repellent and enzyme activities in Culex pipiens L.

et al. 2022

View full text Add to dashboard Cite

Melia azedarach L. (Meliaceae) is a botanical species with focal point of global research for its biological properties. The Melia azedarach tree is distinguished by its rapid growth, its adaptation to different temperate zones, as well as its insecticidal properties. All this made us think of exploiting it in biological control against different stages of mosquitoes. To this end, we aim, through the present work, to evaluate the effectiveness of Melia azedarach extracts against Culex pipiens mosquito. More specifically, our study focuses on determining the chemical composition of Melia almond oil, as well as the larvicidal, ovicidal and repellent activities on Culex pipiens L. mosquito as well as the activities of acetylcholinesterase (AChE) and glutathione-S-transferase (GST). Almond oil was extracted by a Soxhlet and subjected to gas chromatography-mass spectrometry (GC/MS). The yield was found to be 35.17%. The chemical composition revealed the presence of various phytoconstituents. A total of 7 compounds were identified, the main ones being 9,11-Octadecadienoic acid, methyl ester, (E,E)-(79.32%), 9-octadecenoic acid (Z)-, methyl ester (13.24%), hexadecanoic acid and methyl ester (3.69%). The larvicidal bioassays were performed according to the protocol recommended by the World Health Organization with concentrations varying from 20 to 80 mg/L depending on the exposure time (24, 48 and 72 hours). The almond oil exhibited remarkable larvicidal activity against fourth instar larvae and the lethal concentrations were determined (LC 25 = 23.70 mg/L, LC 50 =35.49 mg/L, LC 90 =79.61 mg/L). The results also showed that the oil caused an ovicidal activity with a significant effect on egg hatch. The recorded hatching percentages were respectively 88.79% and 72.40% for the LC 25 and LC 50 , and this compared to the control series. Moreover, this oil exhibited significant repellency against adult mosquitoes. Furthermore, the enzymatic measurements performed on LC 50 and LC 90 treated larvae revealed a neurotoxic activity and a stimulation of the detoxification system as evidenced, respectively, by an inhibition of AChE and induction in GST activity. Overall, our data proved that Melia azedarach almond oil could be considered as a potent biorational alternative to synthetic insecticides for mosquito control.

show abstract

Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)

Cited by 4 publications

References 22 publications

Influence of temperature on the efficiency of Commiphora molmol and Acetylsalicylic acid against Culex pipiens (Diptera: Culicidae)

Influence of temperature on the efficiency of Commiphora molmol and Acetylsalicylic acid against Culex pipiens (Diptera: Culicidae)

Chemical screening, insecticidal and reprotoxic activities of Tecoma stans ethanolic leaf extract against the vector mosquito Culex pipiens

Phytochemical composition of almond oil from Melia azedarach L. and its larvicidal, ovicidal, repellent and enzyme activities in Culex pipiens L.

Contact Info

Product

Resources

About