Effect of Soybean Varieties on Survival and Fecundity of Western Corn Rootworm

Abstract

The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a major pest of corn (Zea mays L.) in North America and has evolved resistance to crop rotation by ovipositing in alternate crops such as soybeans [Glycine max (L.) Merr.]. Through experiments with plants grown in the greenhouse and the field, we tested whether soybeans with resistance to the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), affected survival, fecundity, and consumption of soybean for D. v. virgifera. Soybean varieties tested included those types resistant to A. glycines (Rag1 and rag1/rag3) and a susceptible near isoline of the Rag1 variety. Females were provided with a diet of corn tissue for 4 d after which they were fed a diet of tissue from one of three soybean varieties for 4 d, starved for 4 d, or fed corn tissue. When fed greenhouse grown plants, strains differed significantly in survival and consumption, but consumption did not differ by variety of soybean. Diet treatment only affected fecundity; individuals fed corn continuously had greater fecundity than those individuals fed soybeans. In the experiment with plants grown in the field, leaf consumption differed among strains and individuals fed corn continuously had greater fecundity than the other treatments. Soybean varieties with Rag1 and rag1/rag3 resistance toA. glycines did not appear to affect the fitness of D. v. virgifera. Thus, planting of these A. glycines-resistant soybean varieties should not directly affect the spread of rotation-resistant D. v. virgifera. KeywordsAphis glycines, Diabrotica virgifera virgifera, host plant resistance, Rag genes, rotation resistance RightsThis article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America.This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ent_pubs/184BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Effect of Soybean Varieties on Survival and Fecundity of Western Corn RootwormAuthor (s)

Field-evolved resistance by western corn rootworm to multipleBacillus thuringiensistoxins in transgenic maize

“…In the second type of control, 12 larvae in the late stage of the second instar and in the early stage of the third and final instar were placed into each bioassay container, and then the containers were placed in an incubator for 17 d, after which roots and soil were carefully searched by hand for larvae, pupae, and teneral adults. Larvae for this second control were from a nondiapausing strain of western corn rootworm and were reared to late second and early third instar on mats of maize seedlings (38). Instar was determined by visual inspection of head capsule width and body size using a dissection microscope.…”

Beyond Focal Pests: Impact of a Neonicotinoid Seed Treatment and Resistant Soybean Lines on a Non-Target Arthropod

“…The amino acid composition of leaf tissue from soybean lines with Rag1 genes is different than that of related plants lacking these genes [53]. Even so, consuming plant tissue from soybean lines with Rag genes did not alter fitness of western corn rootworm larvae ( Diabrotica virgifera virgifera LeConte) [54], and other studies [22,55] concluded that the presence of Rag1 genes had little impact on feeding (preference or defoliation) of several soybean pests within the Coleoptera and Lepidoptera, although in the latter study the authors documented reduced caterpillar growth rates. In contrast, Rich and Koch [23] found that brown marmorated stink bugs [ Halyomorpha halys (Stål)] preferred soybeans with Rag1 , and had lower levels of mortality when reared on pods from Rag1 plants.…”

Effects of Rag1 aphid‐resistant soybean on mortality, development, and preference of brown marmorated stink bug

“…() demonstrated that leaf tissue from Rag1 aphid‐resistant soybean retains resistance to A. glycines after detachment from plants. Furthermore, studies evaluating the effect of aphid‐resistant soybean on other insects have relied on detached plant tissue (Lundgren et al., ; Chandrasena et al., ; Dunbar & Gassman, ; Bruner et al., ). IA3027 and IA3027RA1 soybean were grown in 2014, using standard agronomic practices (Bennett et al., ; Kandel, ) and without insecticide application at the University of Minnesota Experiment Station (Saint Paul, MN, USA).…”

“…Defoliating pests (i.e., Coleoptera and Lepidoptera) of soybean have shown a variety of responses in terms of preference and performance when subjected to aphid‐resistant soybean (Chandrasena et al., ; Dunbar & Gassman, ; Bruner et al., ). The response of H. halys or other North American species of stink bugs to aphid‐resistant soybean has not been reported.…”